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Author : Huanbin Zhou,Lanqin Xia,Yong Zhang,Seiichi Toki
Publisher : Frontiers Media SA
Page : 137 pages
File Size : 41,9 Mb
Release : 2021-10-25
Category : Science
ISBN : 9782889715541
Author : Kejian Wang,Shuangxia Jin,Maria Raffaella Ercolano
Publisher : Frontiers Media SA
Page : 248 pages
File Size : 46,9 Mb
Release : 2023-03-07
Category : Science
ISBN : 9782832516850
Author : Takashi Yamamoto
Publisher : Springer
Page : 206 pages
File Size : 53,5 Mb
Release : 2015-01-05
Category : Science
ISBN : 9784431552277
This book serves as an introduction to targeted genome editing, beginning with the background of this rapidly developing field and methods for generation of engineered nucleases. Applications of genome editing tools are then described in detail, in iPS cells and diverse organisms such as mice, rats, marine invertebrates, fish, frogs, and plants. Tools that are mentioned include zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and CRISPR/Cas9, all of which have received much attention in recent years as breakthrough technologies. Genome editing with engineered nucleases allows us to precisely change the target genome of living cells and is a powerful way to control functional genes. It is feasible in almost all organisms ranging from bacteria to plants and animals, as well as in cultured cells such as ES and iPS cells. Various genome modifications have proven successful, including gene knockout and knock-in experiments with targeting vectors and chromosomal editing. Genome editing technologies hold great promise for the future, for example in biomedical research, clinical medicine, and generation of crops and livestock with desirable traits. A wide range of readers will find this book interesting, and with its focus on applications in a variety of organisms and cells, the book will be valuable for life scientists in all fields.
Author : Om Prakash Gupta,Suhas Gorakh Karkute
Publisher : CRC Press
Page : 382 pages
File Size : 40,5 Mb
Release : 2021-07-25
Category : Science
ISBN : 9781000410198
Genome Editing in Plants: Principles and Applications addresses the information of genome editing starting from principles and historical aspects to the latest advancements in the field. As genome-editing technology has emerged as promising and cutting edge, researchers around the world have started producing original research outputs, which have significantly improved our current understanding and potential of this technology. The initial chapters of this book describe different genome-editing tools as well as their principles and applications. Other chapters are dedicated to the present status and future applications of genome-editing techniques in various crop improvement programmes. Some of the advanced applications of CRISPR/Cas tools, such as base editing and RNA detection, along with regulatory aspects of genome-edited crops are described in detail. This book serves as a valuable resource to researchers in the field of crop improvement; graduate and postgraduate students engaged in plant molecular biology and biotechnology; academicians; and policy makers. Key Features: Addresses topics associated with historical development and principles of genome-editing technology Addresses basic mechanisms operating under each genome-editing technology Addresses its application in plants to design crops as per the current and future demands Addresses the regulatory mechanisms of genome-edited crops
Author : Shabir Hussain Wani,Goetz Hensel
Publisher : Springer Nature
Page : 338 pages
File Size : 45,8 Mb
Release : 2022-11-08
Category : Science
ISBN : 9783031080722
Over the last few decades, various techniques have been developed to alter the properties of plants and animals. While the targeted transfer of recombinant DNA into crop plants remains a valuable tool to achieve a desirable breeding outcome, integration of transgenes into the host genome has been random, which in part, leads to reduced acceptance of GMOs by the general population in some parts of the world. Likewise, methods of induced mutagenesis, such as TILLING, have the disadvantage that many mutations are induced per plant, which has to be removed again by expensive backcrossing. Advances in genome sequencing have provided more and more information on differences between susceptible and resistant varieties, which can now be directly targeted and modified using CRISPR/Cas9 technology. By selecting specific gRNAs occurrence of off-target modifications are comparatively low. ZFNs and TALENs- based approaches required re-engineering a new set of assembled polypeptides for every new target site for each experiment. The difficulty in cloning and protein engineering prevented these tools from being broadly adopted by the scientific community. Compared to these technologies, designing the CRISPR toolbox is much simpler and more flexible. CRISPR/Cas9 is versatile, less expensive and highly efficient. It has become the most widely used technology for genome editing in many organisms. Since its inception as a powerful genome-editing tool in late 2012, this breakthrough technology has completely changed how science is performed. The first few chapters in this book introduce the basic concept, design and implementation of CRISPR/Cas9 for different plant systems. They are followed by in-depth discussions on the legal and bio-safety issues accompanying commercialization and patenting of this emerging technology. Lastly, this book covers emerging areas of new tools and potential applications. We believe readers, novice and expert alike, will benefit from this all-in-one resource on genome editing for crop improvement. Chapter 17 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
Author : Deepu Pandita,Anu Pandita
Publisher : CRC Press
Page : 463 pages
File Size : 40,7 Mb
Release : 2023-06-30
Category : Science
ISBN : 9781000771251
With over 820 million people facing hunger in today’s world, the need of the hour is the design of plant varieties with high yield, improved traits, and resistance properties in order to mitigate the detrimental impacts of biotic and abiotic stress conditions on food crops. This volume highlights the use of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR-associated nuclease proteins as a solution for sustainable agriculture. CRISPR/Cas-mediated genome editing (GE) provides a robust, simple, precise, efficient, economical, and revolutionary toolbox that leads to improved plant traits with enhanced yield, quality, and resistance against various stresses, including climate-related stress, insect pests and diseases, pollution-related stresses, etc. Key features of the book: Explains the differences between conventional breeding, genetically modified crops, and genome editing approaches using CRISPR/Cas Introduces the tools of genome editing, such as ZFNS (zinc finger nucleases), mega nucleases, and TLENS (transcription activator-like effector nucleases) Covers the history, origin, discovery, structure, and classification of CRISPR/Cas Examines the databases and computational approaches of CRISPR/Cas Highlights genome editing of important crop plants with CRISPR/Cas systems, including wheat, maize. tomato, Brassica crops, rice, fruits Explores potential applications of CRISPR/Cas systems for climate smart crops
Author : Kaijun Zhao,Rukmini Mishra,Raj Kumar Joshi
Publisher : Springer Nature
Page : 447 pages
File Size : 43,9 Mb
Release : 2022-08-01
Category : Technology & Engineering
ISBN : 9789811906008
This book compiles the relevant information related to genome editing tools and their roles in crop improvement. The book contains a brief introduction about various genome editing tools and their application in major crops. It discusses the genome editing approaches and the strategies used for genome editing in different crops. Some of the chapters cover the detailed methodology of sgRNA design, vector construction and transformation in different crops followed by data analysis. A few chapters focus on the applications of genome editing tools towards crop improvement. This book will be of particular interest to plant biologists working in the field of genome editing and crop breeders. It will provide valuable information and useful material for our readers’ experimental work.
Author : Ashwani Kumar
Publisher : Springer Nature
Page : 871 pages
File Size : 50,5 Mb
Release : 2024-06-30
Category : Electronic
ISBN : 9789819985296
Author : Anonim
Publisher : Academic Press
Page : 264 pages
File Size : 53,5 Mb
Release : 2017-08-31
Category : Science
ISBN : 9780128117446
Gene Editing in Plants, Volume 149 aims to provide the reader with an up-to-date survey of cutting-edge research with gene editing tools and an overview of the implications of this research on the nutritional quality of fruits, vegetables and grains. New chapters in the updated volume include topics relating to Genome Engineering and Agriculture: Opportunities and Challenges, the Use of CRISPR/Cas9 for Crop Improvement in Maize and Soybean, the Use of Zinc-Finger Nucleases for Crop Improvement, Gene Editing in Polyploid Crops: Wheat, Camelina, Canola, Potato, Cotton, Peanut, Sugar Cane, and Citrus, and Gene Editing With TALEN and CRISPR/Cas in Rice. This ongoing serial contain contributions from leading scientists and researchers in the field of gene editing in plants who describe the results of their own research in this rapidly expanding area of science. Shows the importance of revolutionary gene editing technology on plant biology research and its application to agricultural production Provides insight into what may lie ahead in this rapidly expanding area of plant research and development Contains contributions from major leaders in the field of plant gene editing
Author : Anjanabha Bhattacharya,Vilas Parkhi,Bharat Char
Publisher : Springer Nature
Page : 232 pages
File Size : 48,8 Mb
Release : 2020-12-11
Category : Science
ISBN : 9783030420222
This book offers a comprehensive collection of papers on CRISPR/Cas genome editing in connection with agriculture, climate-smart crops, food security, translational research applications, bioinformatics analysis, practical applications in cereals, floriculture crops, engineering plants for abiotic stress resistance, the intellectual landscape, regulatory framework, and policy decisions. Gathering contributions by internationally respected experts in the field of CRISPR/Cas genome editing, the book offers an essential guide for researchers, students, teachers and scientists in academia; policymakers; and public companies, private companies and cooperatives interested in understanding and/or applying CRISPR/Cas genome editing to develop new agricultural products.
Author : Feng Zhang,Holger Puchta,James G. Thomson
Publisher : Springer
Page : 166 pages
File Size : 41,8 Mb
Release : 2015-04-21
Category : Science
ISBN : 9781493925568
Over the past 50 years, biotechnology has been the major driving force for increasing crop productivity. Particularly, advances in plant genetic engineering technologies have opened up vast new opportunities for plant researchers and breeders to create new crop varieties with desirable traits. Recent development of precise genome modification methods, such as targeted gene knock-out/knock-in and precise gene replacement, moves genetic engineering to another level and offers even more potentials for improving crop production. The work provides an overview of the latest advances on precise genomic engineering technologies in plants. Topics include recombinase and engineered nucleases-mediated targeted modification, negative/positive selection-based homologous recombination and oligo nucleotide-mediated recombination. Finally, challenges and impacts of the new technologies on present regulations for genetic modification organisms (GMOs) will be discussed.
Author : Aftab Ahmad,Sultan Habibullah Khan,Zulqurnain Khan
Publisher : Springer Nature
Page : 311 pages
File Size : 52,5 Mb
Release : 2021-01-08
Category : Medical
ISBN : 9789811571428
This book compiles the latest applications of the cutting-edge gene editing tool CRISPR/Cas in the area of crop improvement. It begins with an introduction to the technique and its application in crop plants. Next, it gives an updated overview of available delivery methods, design tools and resources in CRISPR/Cas. The book subsequently reviews the applications of CRISPR/Cas in connection with e.g. insect stress, disease stress, abiotic stress, nutritional and yield improvement in crop plants, etc. It also discusses the various regulatory, ethical and social aspects of the technique that must be kept in mind when designing experiments. In closing, the book summarizes the status quo and outlines future prospects for the tool in crop improvement and food security. Given its scope, the book will especially benefit students and researchers in food science, biotechnology, agriculture and the plant sciences.
Author : Sandeep Kumar,Hiroshi Ezura,Vladimir Nekrasov,Linda Ann Rymarquis
Publisher : Frontiers Media SA
Page : 148 pages
File Size : 49,8 Mb
Release : 2021-05-28
Category : Science
ISBN : 9782889668151
Author : Alvina Gul
Publisher : Academic Press
Page : 0 pages
File Size : 51,6 Mb
Release : 2024-08-04
Category : Science
ISBN : 044326614X
Targeted Genome Engineering via CRISPR/Cas9 in Plants provides in-depth insights into the use of the emerging “CRISPR/Cas9” technology for precise genome editing. This technology has revolutionized plant science research particularly for crop improvement owing to its simplicity and efficiency. The book provides a wide range of CRISPR/Cas9 gene editing techniques for a variety of plants. Chapters include the latest applications of CRISPR/Cas9 system in connection with abiotic stresses, biotic stresses, biofortification, yield improvement, disease modelling and prognosis and molecular diagnosis. Targeted Genome Engineering via CRISPR/Cas9 in Plants also evaluates various regulatory and ethical aspects that must be considered when implementing the CRISPR/Cas9 approach. This book is a valuable resource for professionals and researchers, as it provides effective CRISPR/Cas9-based strategies for sustainable agriculture and treatment of various diseases.
Author : Aftab Ahmad,Neil E. Hoffman,Michael George Kepler Jones,Baohong Zhang
Publisher : Frontiers Media SA
Page : 153 pages
File Size : 47,7 Mb
Release : 2024-02-07
Category : Science
ISBN : 9782832544273
