Ultra High Performance Liquid Chromatography And Its Applications

Author : Q. Alan Xu
Publisher : John Wiley & Sons
Page : 342 pages
File Size : 52,6 Mb
Release : 2013-04-01
Category : Technology & Engineering
ISBN : 9781118533987

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Ultra-High Performance Liquid Chromatography and Its Applications by Q. Alan Xu Pdf

Explores both the benefits and limitations of new UHPLC technology High performance liquid chromatography (HPLC) has been widely used in analytical chemistry and biochemistry to separate, identify, and quantify compounds for decades. The science of liquid chromatography, however, was revolutionized a few years ago with the advent of ultra-high performance liquid chromatography (UHPLC), which made it possible for researchers to analyze sample compounds with greater speed, resolution, and sensitivity. Ultra-High Performance Liquid Chromatography and Its Applications enables readers to maximize the performance of UHPLC as well as develop UHPLC methods tailored to their particular research needs. Readers familiar with HPLC methods will learn how to transfer these methods to a UHPLC platform and vice versa. In addition, the book explores a variety of UHPLC applications designed to support research in such fields as pharmaceuticals, food safety, clinical medicine, and environmental science. The book begins with discussions of UHPLC method development and method transfer between HPLC and UHPLC platforms. It then examines practical aspects of UHPLC. Next, the book covers: Coupling UHPLC with mass spectrometry Potential of shell particles in fast liquid chromatography Determination of abused drugs in human biological matrices Analyses of isoflavones and flavonoids Therapeutic protein characterization Analysis of illicit drugs The final chapter of the book explores the use of UHPLC in drug metabolism and pharmacokinetics studies for traditional Chinese medicine. With its frank discussions of UHPLC's benefits and limitations, Ultra-High Performance Liquid Chromatography and Its Applications equips analytical scientists with the skills and knowledge needed to take full advantage of this new separation technology.

Author : R. Hamilton
Publisher : Springer Science & Business Media
Page : 256 pages
File Size : 47,9 Mb
Release : 2012-12-06
Category : Science
ISBN : 9789400959385

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Introduction to high performance liquid chromatography by R. Hamilton Pdf

Since the first edition of this book the major advances have been in column packings, where over ninety per cent of separations are now performed using chemically bonded microparticulate packings, and in instrumentation. The use of microprocessor control has brought about a rationalization of mobile phase delivery systems and in detectors, the introduction of electrochemical and spectrophotometric detection other than in the ultra-violet region, has widened the field of applications and the sensitivity of the technique. The use of ion pair chromatography has increased at the expense of ion-exchange and this together with the improvements in detectors has greatly increased the appli cation of the technique in the biomedical field. These advances are described together with the established methods to enable the beginner to carry out a satisfactory separation and to gain the experience necessary for the full exploitation of the technique. R. J. Hamilton P. A. Sewell Liverpool,1981 1 Introduction to high performance liquid chromatography 1. 1 Introduction Chromatography in its many forms is widely used as a separative and an analytical technique. Gas chromatography since its introduction by James and Martin [1] has been pre-eminent in the field. Uquid chromatography in the of paper, thin-layer, ion-exchange, and exclusion (gel permeation and gel form filtration) chromatography had not been able to achieve the same success, mainly because of the poor efficiences and the long analysis times arising from the low mobile phase flow rates.

Author : Mu Naushad,Mohammad Rizwan Khan
Publisher : CRC Press
Page : 464 pages
File Size : 42,8 Mb
Release : 2014-03-18
Category : Science
ISBN : 9781466591554

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Ultra Performance Liquid Chromatography Mass Spectrometry by Mu Naushad,Mohammad Rizwan Khan Pdf

This book presents a unique collection of up-to-date UPLC-MS/MS (ultra performance liquid chromatography-tandem mass spectrometric) methods for the separation and quantitative determination of pesticides, capsaicinoids, heterocyclic amines, aflatoxin, perfluorochemicals, acrylamide, procyanidins and alkaloids, lactose content, phenolic compounds, vitamins, and aroma and flavor compounds in a wide variety of foods and food products. With contributions by experts in interdisciplinary fields, this reference offers practical information for readers in research and development, production, and routing analysis of foods and food products.

Author : Ivan Lucero
Publisher : Unknown
Page : 0 pages
File Size : 48,6 Mb
Release : 2018
Category : Analytical chemistry
ISBN : 1536135437

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High-performance Liquid Chromatography by Ivan Lucero Pdf

In this collection, the authors discuss the way in which it is possible to detect the low concentrations at which toxic compounds and metabolites are present in specimens due to the huge development of chromatographic techniques. Chromatography is an analytical procedure used for the separation of compounds/drugs and its most commonly used types are based on either high performance liquid chromatography or gas chromatography, both coupled to mass spectrometry. As such, the advances in liquid chromatography coupled to different detectors, particularly mass spectrometry, giving examples of its applications in the areas of clinical and forensic toxicology. The authors present the importance of using high-performance liquid chromatography in analytical chemistry, as well as the positive aspects related to the use of chemometric approaches for the optimization of processes during the development of analytical methods. Case studies about real applications of chemometric tools focusing on the analysis of water samples polluted with a mixture of polycyclic aromatic hydrocarbons are presented. Next, sample preparation is considered as a difficult part of any analytical procedure in which the chemical compounds are present at low concentrations in complex matrices. Graphene based materials have been more and more popular in the procedure of sample preparation. Graphene is a material that has good physical-chemical properties, high fracture stretch, ultra-high specific surface area with nanosheets morphology and good mechanical and thermal stability. In the concluding study, the authors present a simple, economic and environmental friendly modification of stationary phase by self-assembly positive charged photosensitive diazoresin. The preparation and modification process of the chromatographic stationary phases is discussed. Additionally, the separation performance of chromatographic stationary phase is also discussed.

Author : Sandie Lindsay
Publisher : John Wiley & Sons
Page : 368 pages
File Size : 42,9 Mb
Release : 1992-07-27
Category : Science
ISBN : 0471931152

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High Performance Liquid Chromatography by Sandie Lindsay Pdf

High performance liquid chromatography is the most powerful of all the chromatographic techniques, often achieving separations and analyses that would be difficult or impossible with other forms of chromatography. This study and training text examines the concepts and techniques used in this field. A selection of literature available from equipment manufacturers is included along with a brief review of some more specialized topics.

Author : S. Pryde,M.T. Gilbert
Publisher : Springer Science & Business Media
Page : 288 pages
File Size : 40,6 Mb
Release : 1979-01-31
Category : Science
ISBN : 0412142201

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Applications of High Performance Liquid Chromatography by S. Pryde,M.T. Gilbert Pdf

Author : Oscar Nuñez,Sonia Sentellas,Mercè Granados,Javier Saurina
Publisher : Unknown
Page : 0 pages
File Size : 46,9 Mb
Release : 2023
Category : High performance liquid chromatography
ISBN : 1837685673

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High Performance Liquid Chromatography by Oscar Nuñez,Sonia Sentellas,Mercè Granados,Javier Saurina Pdf

Author : Yuegang Zuo
Publisher : Nova Science Publishers
Page : 0 pages
File Size : 47,9 Mb
Release : 2014
Category : Chromatographic analysis
ISBN : 1629488542

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High-performance Liquid Chromatography (HPLC) by Yuegang Zuo Pdf

Includes bibliographical references and index.

Author : Michal Holcapek,Wm. Craig Byrdwell
Publisher : Elsevier
Page : 520 pages
File Size : 40,9 Mb
Release : 2017-09-07
Category : Science
ISBN : 9780128117330

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Handbook of Advanced Chromatography /Mass Spectrometry Techniques by Michal Holcapek,Wm. Craig Byrdwell Pdf

Handbook of Advanced Chromatography /Mass Spectrometry Techniques is a compendium of new and advanced analytical techniques that have been developed in recent years for analysis of all types of molecules in a variety of complex matrices, from foods to fuel to pharmaceuticals and more. Focusing on areas that are becoming widely used or growing rapidly, this is a comprehensive volume that describes both theoretical and practical aspects of advanced methods for analysis. Written by authors who have published the foundational works in the field, the chapters have an emphasis on lipids, but reach a broader audience by including advanced analytical techniques applied to a variety of fields. Handbook of Advanced Chromatography / Mass Spectrometry Techniques is the ideal reference for those just entering the analytical fields covered, but also for those experienced analysts who want a combination of an overview of the techniques plus specific and pragmatic details not often covered in journal reports. The authors provide, in one source, a synthesis of knowledge that is scattered across a multitude of literature articles. The combination of pragmatic hints and tips with theoretical concepts and demonstrated applications provides both breadth and depth to produce a valuable and enduring reference manual. It is well suited for advanced analytical instrumentation students as well as for analysts seeking additional knowledge or a deeper understanding of familiar techniques. Includes UHPLC, HILIC, nano-liquid chromatographic separations, two-dimensional LC-MS (LCxLC), multiple parallel MS, 2D-GC (GCxGC) methodologies for lipids analysis, and more Contains both practical and theoretical knowledge, providing core understanding for implementing modern chromatographic and mass spectrometric techniques Presents chapters on the most popular and fastest-growing new techniques being implemented in diverse areas of research

Author : Michael W. Dong
Publisher : John Wiley & Sons
Page : 410 pages
File Size : 47,5 Mb
Release : 2019-07-23
Category : Science
ISBN : 9781119313762

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HPLC and UHPLC for Practicing Scientists by Michael W. Dong Pdf

A concise yet comprehensive reference guide on HPLC/UHPLC that focuses on its fundamentals, latest developments, and best practices in the pharmaceutical and biotechnology industries Written for practitioners by an expert practitioner, this new edition of HPLC and UHPLC for Practicing Scientists adds numerous updates to its coverage of high-performance liquid chromatography, including comprehensive information on UHPLC (ultra-high-pressure liquid chromatography) and the continuing migration of HPLC to UHPLC, the modern standard platform. In addition to introducing readers to HPLC’s fundamentals, applications, and developments, the book describes basic theory and terminology for the novice, and reviews relevant concepts, best practices, and modern trends for the experienced practitioner. HPLC and UHPLC for Practicing Scientists, Second Edition offers three new chapters. One is a standalone chapter on UHPLC, covering concepts, benefits, practices, and potential issues. Another examines liquid chromatography/mass spectrometry (LC/MS). The third reviews at the analysis of recombinant biologics, particularly monoclonal antibodies (mAbs), used as therapeutics. While all chapters are revised in the new edition, five chapters are essentially rewritten (HPLC columns, instrumentation, pharmaceutical analysis, method development, and regulatory aspects). The book also includes problem and answer sections at the end of each chapter. Overviews fundamentals of HPLC to UHPLC, including theories, columns, and instruments with an abundance of tables, figures, and key references Features brand new chapters on UHPLC, LC/MS, and analysis of recombinant biologics Presents updated information on the best practices in method development, validation, operation, troubleshooting, and maintaining regulatory compliance for both HPLC and UHPLC Contains major revisions to all chapters of the first edition and substantial rewrites of chapters on HPLC columns, instrumentation, pharmaceutical analysis, method development, and regulatory aspects Includes end-of-chapter quizzes as assessment and learning aids Offers a reference guide to graduate students and practicing scientists in pharmaceutical, biotechnology, and other industries Filled with intuitive explanations, case studies, and clear figures, HPLC and UHPLC for Practicing Scientists, Second Edition is an essential resource for practitioners of all levels who need to understand and utilize this versatile analytical technology. It will be a great benefit to every busy laboratory analyst and researcher.

Author : Szabolcs Fekete,Imre Molnar
Publisher : World Scientific
Page : 364 pages
File Size : 45,6 Mb
Release : 2018-08-01
Category : Science
ISBN : 9781786345479

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Software-assisted Method Development In High Performance Liquid Chromatography by Szabolcs Fekete,Imre Molnar Pdf

'The book is a useful contribution in the field of HPLC, and may represent a valuable tool for chromatography practitioners in different fields, as well as teachers and instructors. The 12 chapters provide comprehensive insights of current day retention and resolution modelling in HPLC, and its applications for small and large molecule analysis. It may be a useful reference for specialists in pharmaceuticals but not limited to … It may be a valuable resource to assist scientists involved in method development, aiming to achieve the best results with reduced costs, time, and efforts.'Analytical and Bioanalytical ChemistryThis handbook gives a general overview of the possibilities in recent developments in chromatographic retention modeling. As a result of the latest developments in modeling software, several new features are now accessible, opening a new level in HPLC method development.Many of these current possibilities in software assisted liquid chromatographic method modeling for analytical purposes are presented. Several modes of chromatography, including Reversed-Phase Liquid Chromatography (RPLC), Ion Exchange Chromatography (IEX), Hydrophobic Interaction Chromatography (HIC), and Hydrophilic Interaction Liquid Chromatography (HILIC) are explained in detail. For all these chromatographic modes, the most important variables for tuning retention and selectivity are exposed.Beside the industrial and practical benefits of retention modeling, the possibilities in teaching and education are also illustrated. Finally, numerous representative industrial examples are shown, to highlight the benefits, time and cost savings offered by state-of-the-art software assisted HPLC method development.

Author : John N. Done,J. H. Knox,John H. Knox,Joël Loheac
Publisher : Wiley-Interscience
Page : 256 pages
File Size : 53,9 Mb
Release : 1974
Category : Science
ISBN : UOM:39015016084058

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Applications of High-speed Liquid Chromatography by John N. Done,J. H. Knox,John H. Knox,Joël Loheac Pdf

Author : Omar Al Sayed Omar,Moustafa A. Khalifa
Publisher : Walter de Gruyter GmbH & Co KG
Page : 322 pages
File Size : 40,9 Mb
Release : 2022-02-21
Category : Science
ISBN : 9783110529456

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High Performance Liquid Chromatography by Omar Al Sayed Omar,Moustafa A. Khalifa Pdf

The book provides an indispensable guide on how to use HPLC in pharmaceutical analysis and drug control. Following a hands-on approach, the authors give practical advices how to prepare stationary and mobile phases, choose a suitable detector and set up an HPLC analysis. The publication gives insight into the key pharmaceutical applications of HPLC and the latest requirements of the major regulatory agencies.

Author : Phyllis R. Brown,Richard A. Hartwick
Publisher : Wiley-Interscience
Page : 710 pages
File Size : 51,7 Mb
Release : 1989-01-19
Category : Science
ISBN : UOM:39015038990811

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High Performance Liquid Chromatography by Phyllis R. Brown,Richard A. Hartwick Pdf

Detectors for Liquid Chromatography Edited by Edward S. Yeung Written by an expert in the field, this comprehensive guide explains the basic principles behind detector response instrumentation and selected applications. Early chapters cover absorption detectors for high performance liquid chromatography, FTIR detection, indirect absorbance detectors, fluorometric detection, and polarimetric detectors. Coverage continues with detection based on electrical and electrochemical measurements, mass spectrometry as an online detector for HPLC, and miscellaneous methods. 1986 0 471-82169-1 366 pp. Small Bore High Performance Liquid Chromatography Edited by Raymond R. W. Scott A state-of-the-art guide that demonstrates how to design, construct, and pack optimized small bore columns, the center of any chromatography system. Case examples show the use of these columns for high resolution, very fast analysis, and special methods for molecular weight determinations. Applications from a wide range of industrial and forensic analyses aid in developing sophistication in a number of useful techniques. The book provides essential information on topics such as calculating the minimum column radius, detectors, and molecular diffusion. Includes 92 illustrations and 14 tables to enhance explanations of microbore HPLC methods. 1984 0 471-80052-X 271 pp. Reversed-Phase High Performance Liquid Chromatography: Theory, Practice, and Biomedical Applications Ante M. Krstulovic and Phyllis R. Brown Reversed-phase liquid chromatography has increased tremendously in popularity over the past ten years. Estimates show that more than 80% of all HPLC separations are performed using this technique. This book covers both theoretical aspects of RPLC and practical information needed in diverse areas of research; it also contains a review of the RPLC applications in the biomedical/biochemical field, with references and collateral readings. Material is presented in a practical, problem-solving manner and should be immensely useful in theoretical aspects of RPLC and all areas of scientific research, particularly the biomedical/biochemical field, where RPLC has made its largest impact. 1982 0 471-05369-4 296 pp.

Author : Oscar Núñez,Sonia Sentellas,Mercè Granados,Javier Saurina
Publisher : Intechopen
Page : 0 pages
File Size : 53,9 Mb
Release : 2023-05
Category : Electronic
ISBN : 1837685657

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High Performance Liquid Chromatography by Oscar Núñez,Sonia Sentellas,Mercè Granados,Javier Saurina Pdf

Increasing interest in topics related to health and quality of life in recent years has led to a growing need in food, environmental and bioanalytical research for high-throughput separation techniques able to cope with the qualitative/quantitative determination of a large number of compounds in very complex matrices. High-performance liquid chromatography (HPLC) is a well-established separation technique widely employed in many fields. The versatility of chromatographic separation modes, coupled with low-resolution and high-resolution mass spectrometry, makes HPLC among the best options to solve emerging analytical problems. This book provides an overview of new advances in high-performance liquid chromatography and its applications in different fields.