Hplc And Uhplc For Practicing Scientists

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HPLC and UHPLC for Practicing Scientists

Michael W. Dong
HPLC and UHPLC for Practicing Scientists Book PDF

Author : Michael W. Dong
Publisher : John Wiley & Sons
Page : 410 pages
File Size : 44,9 Mb
Release : 2019-07-23
Category : Science
ISBN : 9781119313762

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HPLC and UHPLC for Practicing Scientists by Michael W. Dong Pdf

A concise yet comprehensive reference guide on HPLC/UHPLC that focuses on its fundamentals, latest developments, and best practices in the pharmaceutical and biotechnology industries Written for practitioners by an expert practitioner, this new edition of HPLC and UHPLC for Practicing Scientists adds numerous updates to its coverage of high-performance liquid chromatography, including comprehensive information on UHPLC (ultra-high-pressure liquid chromatography) and the continuing migration of HPLC to UHPLC, the modern standard platform. In addition to introducing readers to HPLC’s fundamentals, applications, and developments, the book describes basic theory and terminology for the novice, and reviews relevant concepts, best practices, and modern trends for the experienced practitioner. HPLC and UHPLC for Practicing Scientists, Second Edition offers three new chapters. One is a standalone chapter on UHPLC, covering concepts, benefits, practices, and potential issues. Another examines liquid chromatography/mass spectrometry (LC/MS). The third reviews at the analysis of recombinant biologics, particularly monoclonal antibodies (mAbs), used as therapeutics. While all chapters are revised in the new edition, five chapters are essentially rewritten (HPLC columns, instrumentation, pharmaceutical analysis, method development, and regulatory aspects). The book also includes problem and answer sections at the end of each chapter. Overviews fundamentals of HPLC to UHPLC, including theories, columns, and instruments with an abundance of tables, figures, and key references Features brand new chapters on UHPLC, LC/MS, and analysis of recombinant biologics Presents updated information on the best practices in method development, validation, operation, troubleshooting, and maintaining regulatory compliance for both HPLC and UHPLC Contains major revisions to all chapters of the first edition and substantial rewrites of chapters on HPLC columns, instrumentation, pharmaceutical analysis, method development, and regulatory aspects Includes end-of-chapter quizzes as assessment and learning aids Offers a reference guide to graduate students and practicing scientists in pharmaceutical, biotechnology, and other industries Filled with intuitive explanations, case studies, and clear figures, HPLC and UHPLC for Practicing Scientists, Second Edition is an essential resource for practitioners of all levels who need to understand and utilize this versatile analytical technology. It will be a great benefit to every busy laboratory analyst and researcher.

Modern HPLC for Practicing Scientists

Michael W. Dong
Modern HPLC for Practicing Scientists Book PDF

Author : Michael W. Dong
Publisher : John Wiley & Sons
Page : 328 pages
File Size : 44,7 Mb
Release : 2016-04-06
Category : Science
ISBN : 9781119293606

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Modern HPLC for Practicing Scientists by Michael W. Dong Pdf

A comprehesive yet concise guide to Modern HPLC Written for practitioners by a practitioner, Modern HPLC for Practicing Scientists is a concise text which presents the most important High-Performance Liquid Chromatography (HPLC) fundamentals, applications, and developments. It describes basic theory and terminology for the novice, and reviews relevant concepts, best practices, and modern trends for the experienced practitioner. Moreover, the book serves well as an updated reference guide for busy laboratory analysts and researchers. Topics covered include: HPLC operation Method development Maintenance and troubleshooting Modern trends in HPLC such as quick-turnaround and "greener" methods Regulatory aspects While broad in scope, this book focuses particularly on reversed-phase HPLC, the most common separation mode, and on applications for the pharmaceutical industry, the largest user segment. Accessible to both novice and intermedate HPLC users, information is delivered in a straightforward manner illustrated with an abundance of diagrams, chromatograms, tables, and case studies, and supported with selected key references and Web resources. With intuitive explanations and clear figures, Modern HPLC for Practicing Scientists is an essential resource for practitioners of all levels who need to understand and utilize this versatile analytical technology.

Modern HPLC for Practicing Scientists

Michael W. Dong
Modern HPLC for Practicing Scientists Book PDF

Author : Michael W. Dong
Publisher : Wiley-Interscience
Page : 304 pages
File Size : 44,6 Mb
Release : 2006-06-12
Category : Science
ISBN : 047172789X

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Modern HPLC for Practicing Scientists by Michael W. Dong Pdf

A comprehesive yet concise guide to Modern HPLC Written for practitioners by a practitioner, Modern HPLC for Practicing Scientists is a concise text which presents the most important High-Performance Liquid Chromatography (HPLC) fundamentals, applications, and developments. It describes basic theory and terminology for the novice, and reviews relevant concepts, best practices, and modern trends for the experienced practitioner. Moreover, the book serves well as an updated reference guide for busy laboratory analysts and researchers. Topics covered include: HPLC operation Method development Maintenance and troubleshooting Modern trends in HPLC such as quick-turnaround and "greener" methods Regulatory aspects While broad in scope, this book focuses particularly on reversed-phase HPLC, the most common separation mode, and on applications for the pharmaceutical industry, the largest user segment. Accessible to both novice and intermedate HPLC users, information is delivered in a straightforward manner illustrated with an abundance of diagrams, chromatograms, tables, and case studies, and supported with selected key references and Web resources. With intuitive explanations and clear figures, Modern HPLC for Practicing Scientists is an essential resource for practitioners of all levels who need to understand and utilize this versatile analytical technology.

Practical HPLC Method Development

Lloyd R. Snyder,Joseph J. Kirkland,Joseph L. Glajch
Practical HPLC Method Development Book PDF

Author : Lloyd R. Snyder,Joseph J. Kirkland,Joseph L. Glajch
Publisher : John Wiley & Sons
Page : 665 pages
File Size : 49,7 Mb
Release : 2012-12-03
Category : Science
ISBN : 9781118591512

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Practical HPLC Method Development by Lloyd R. Snyder,Joseph J. Kirkland,Joseph L. Glajch Pdf

This revision brings the reader completely up to date on the evolving methods associated with increasingly more complex sample types analyzed using high-performance liquid chromatography, or HPLC. The book also incorporates updated discussions of many of the fundamental components of HPLC systems and practical issues associated with the use of this analytical method. This edition includes new or expanded treatments of sample preparation, computer assisted method development, as well as biochemical samples, and chiral separations.

HPLC for Pharmaceutical Scientists

Yuri V. Kazakevich,Rosario LoBrutto
HPLC for Pharmaceutical Scientists Book PDF

Author : Yuri V. Kazakevich,Rosario LoBrutto
Publisher : John Wiley & Sons
Page : 1136 pages
File Size : 40,5 Mb
Release : 2007-02-16
Category : Science
ISBN : 9780470087947

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HPLC for Pharmaceutical Scientists by Yuri V. Kazakevich,Rosario LoBrutto Pdf

HPLC for Pharmaceutical Scientists is an excellent book for both novice and experienced pharmaceutical chemists who regularly use HPLC as an analytical tool to solve challenging problems in the pharmaceutical industry. It provides a unified approach to HPLC with an equal and balanced treatment of the theory and practice of HPLC in the pharmaceutical industry. In-depth discussion of retention processes, modern HPLC separation theory, properties of stationary phases and columns are well blended with the practical aspects of fast and effective method development and method validation. Practical and pragmatic approaches and actual examples of effective development of selective and rugged HPLC methods from a physico-chemical point of view are provided. This book elucidates the role of HPLC throughout the entire drug development process from drug candidate inception to marketed drug product and gives detailed specifics of HPLC application in each stage of drug development. The latest advancements and trends in hyphenated and specialized HPLC techniques (LC-MS, LC-NMR, Preparative HPLC, High temperature HPLC, high pressure liquid chromatography) are also discussed.

Introduction to Modern Liquid Chromatography

Lloyd R. Snyder,Joseph J. Kirkland,John W. Dolan
Introduction to Modern Liquid Chromatography Book PDF

Author : Lloyd R. Snyder,Joseph J. Kirkland,John W. Dolan
Publisher : John Wiley & Sons
Page : 831 pages
File Size : 47,6 Mb
Release : 2011-09-20
Category : Science
ISBN : 9781118210390

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Introduction to Modern Liquid Chromatography by Lloyd R. Snyder,Joseph J. Kirkland,John W. Dolan Pdf

The latest edition of the authoritative reference to HPLC High-performance liquid chromatography (HPLC) is today the leading technique for chemical analysis and related applications, with an ability to separate, analyze, and/or purify virtually any sample. Snyder and Kirkland's Introduction to Modern Liquid Chromatography has long represented the premier reference to HPLC. This Third Edition, with John Dolan as added coauthor, addresses important improvements in columns and equipment, as well as major advances in our understanding of HPLC separation, our ability to solve problems that were troublesome in the past, and the application of HPLC for new kinds of samples. This carefully considered Third Edition maintains the strengths of the previous edition while significantly modifying its organization in light of recent research and experience. The text begins by introducing the reader to HPLC, its use in relation to other modern separation techniques, and its history, then leads into such specific topics as: The basis of HPLC separation and the general effects of different experimental conditions Equipment and detection The column—the "heart" of the HPLC system Reversed-phase separation, normal-phase chromatography, gradient elution, two-dimensional separation, and other techniques Computer simulation, qualitative and quantitative analysis, and method validation and quality control The separation of large molecules, including both biological and synthetic polymers Chiral separations, preparative separations, and sample preparation Systematic development of HPLC separations—new to this edition Troubleshooting tricks, techniques, and case studies for both equipment and chromatograms Designed to fulfill the needs of the full range of HPLC users, from novices to experts, Introduction to Modern Liquid Chromatography, Third Edition offers the most up-to-date, comprehensive, and accessible survey of HPLC methods and applications available.

Principles and Practice of Modern Chromatographic Methods

Kevin Robards,Danielle Ryan
Principles and Practice of Modern Chromatographic Methods Book PDF

Author : Kevin Robards,Danielle Ryan
Publisher : Academic Press
Page : 532 pages
File Size : 51,7 Mb
Release : 2021-12-03
Category : Science
ISBN : 9780128220979

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Principles and Practice of Modern Chromatographic Methods by Kevin Robards,Danielle Ryan Pdf

Principles and Practice of Modern Chromatographic Methods, Second Edition takes a comprehensive, unified approach in its presentation of chromatographic techniques. Like the first edition, the book provides a scientifically rigid, but easy-to-follow presentation of chromatography concepts that begins with the purpose and intent of chromatographic theory - the “what and why that are left out of other books attempting to cover these principles. This fully revised second edition brings the content up-to-date, covering recent developments in several new sections and an additional chapter on composite methods. New topics include sample profiling, sample preparation, sustainable green chemistry, 2D chromatography, miniaturization/nano-LC, HILIC, and more. Contains thorough chapters that begin with an updated schematic overview and a visual representation of the content Avoids the obfuscation of different terminologies and classification systems that are prevalent in the area, such as the relationship between liquid chromatography and column chromatography Provides integrated and comprehensive topic coverage based on chromatographic bibliometrics and survey reports on the relative usage of chromatographic techniques

The HPLC Expert II

Stavros Kromidas
The HPLC Expert II Book PDF

Author : Stavros Kromidas
Publisher : John Wiley & Sons
Page : 380 pages
File Size : 48,5 Mb
Release : 2017-04-10
Category : Science
ISBN : 9783527339723

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The HPLC Expert II by Stavros Kromidas Pdf

How can I use my HPLC/UHPLC equipment in an optimal way, where are the limitations of the technique? These questions are discussed in detail in the sequel of the successful "HPLC Expert" in twelve chapters written by experts in the respective fields. The topics encompass - complementary to the first volume - typical HPLC users' problems and questions such as gradient optimization and hyphenated techniques (LC-MS). An important key aspect of the book is UHPLC: For which analytical problem is it essential, what should be considered? Besides presentation of latest developments directly from the main manufacturers, also UHPLC users and independent service engineers impart their knowledge. Consistent with the target groups, the level is advanced, but the emphasis is on practical applications.

UHPLC in Life Sciences

Davy Guillarme,Jean-Luc Veuthey
UHPLC in Life Sciences Book PDF

Author : Davy Guillarme,Jean-Luc Veuthey
Publisher : Royal Society of Chemistry
Page : 484 pages
File Size : 44,6 Mb
Release : 2015-11-09
Category : Science
ISBN : 9781782626152

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UHPLC in Life Sciences by Davy Guillarme,Jean-Luc Veuthey Pdf

Since its commercial introduction in 2004, UHPLC (Ultra-High Performance Liquid Chromatography) has begun to replace conventional HPLC in academia and industry and interest in this technique continues to grow. Both the increases in speed and resolution make this an attractive method; particularly to the life sciences and more than 1500 papers have been written on this strongly-evolving topic to date. This book provides a solid background on how to work with UHPLC and its application to the life sciences. The first part of the book covers the basics of this approach and the specifics of a UHPLC system, providing the reader with a solid background to working properly with such a system. The second part examines the application of UHPLC to the life sciences, with a focus on drug analysis strategies. UHPLC-MS, a key technique in pharmaceutical and toxicological analyses, is also examined in detail. The editors (Davy Guillarme and Jean-Luc Veuthey) were some of the earliest adopters of UHPLC and have published and lectured extensively on this topic. Between them they have brought together an excellent team of contributors from Europe and the United States, presenting a wealth of expertise and knowledge. This book is an essential handbook for anyone wishing to adopt an UHPLC system in either an academic or industrial setting and will benefit postgraduate students and experienced workers alike.

High Performance Liquid Chromatography

W.J. Lough,I.W. Wainer
High Performance Liquid Chromatography Book PDF

Author : W.J. Lough,I.W. Wainer
Publisher : CRC Press
Page : 292 pages
File Size : 41,6 Mb
Release : 1995-09-30
Category : Science
ISBN : 0751400769

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High Performance Liquid Chromatography by W.J. Lough,I.W. Wainer Pdf

High performance liquid chromatography (HPLC) has long been recognized as one of the most useful and versatile analytical techniques. It has now progressed from being a highly expensive method of analysis to a routine technique with wide applications. Consequently there is a requirement in many chemistry and chemistry-related courses for students to acquire a detailed understanding of the principles and practice of HPLC. Written in a manner suitable for undergraduate students studying analytical chemistry and learning about chromatographic analytical techniques applied to pharmaceutical analysis, biochemistry and related disciplines, High-performance Liquid Chromatography: Fundamental Principles and Practice introduces the fundamentals of HPLC. Loosely structured in three parts, the text begins with a thorough introduction of the subject and then progresses through the essential knowledge of the instrumentation needed for HPLC. The final part covers with the applications of HPLC in real-world situations. Developed by a team of international experts from a wide cross-section of disciplines, the text is relevant to a wide range of courses.

Practical High-Performance Liquid Chromatography

Veronika R. Meyer
Practical High Performance Liquid Chromatography Book PDF

Author : Veronika R. Meyer
Publisher : John Wiley & Sons
Page : 434 pages
File Size : 40,8 Mb
Release : 2010-05-17
Category : Science
ISBN : 9780470682180

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Practical High-Performance Liquid Chromatography by Veronika R. Meyer Pdf

Jump into the HPLC adventure! Three decades on from publication of the 1st German edition of Veronika Meyer's book on HPLC, this classic text remains one of the few titles available on general HPLC aimed at practitioners. New sections on the following topics have been included in this fifth edition: Comparison of HPLC with capillary electrophoresis How to obtain peak capacity van Deemter curves and other coherences Hydrophilic interaction chromatography Method transfer Comprehensive two-dimensional HPLC Fast separations at 1000 bar HPLC with superheated water In addition, two chapters on the instrument test and troubleshooting in the appendix have been updated and expanded by Bruno E. Lendi, and many details have been improved and numerous references added. A completely new chapter is presented on quality assurance covering: Is it worth the effort? Verification with a second method Method validation Standard operating procedures Measurement uncertainty Qualifications, instrument test, and system suitability test The quest for quality Reviews of earlier editions "That this text is written by an expert in both the practice and teaching of HPLC is evident from the first paragraph....not only an enjoyable, fascinating and easy read, but a truly excellent text that has and will serve many teachers, students and practitioners very well." —The Analyst “…provides essential information on HPLC for LC practitioners in academia, industry, government, and research laboratories…a valuable introduction." - American Journal of Therapeutics

Material Characterization Techniques and Applications

Euth Ortiz Ortega,Hamed Hosseinian,Ingrid Berenice Aguilar Meza,María José Rosales López,Andrea Rodríguez Vera,Samira Hosseini
Material Characterization Techniques and Applications Book PDF

Author : Euth Ortiz Ortega,Hamed Hosseinian,Ingrid Berenice Aguilar Meza,María José Rosales López,Andrea Rodríguez Vera,Samira Hosseini
Publisher : Springer Nature
Page : 314 pages
File Size : 53,9 Mb
Release : 2022-02-12
Category : Science
ISBN : 9789811695698

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Material Characterization Techniques and Applications by Euth Ortiz Ortega,Hamed Hosseinian,Ingrid Berenice Aguilar Meza,María José Rosales López,Andrea Rodríguez Vera,Samira Hosseini Pdf

This book presents commonly applied characterization techniques in material science, their brief history and origins, mechanism of operation, advantages and disadvantages, their biosensing applications, and troubleshooting for each technique, while addressing the challenges researchers face when working with these techniques. The book dedicates its focus to identifying physicochemical and electrochemical nature of materials including analyses of morphology, mass spectrometry, and topography, as well as the characterization of elemental, structural, thermal, wettability, electrochemical, and chromatography properties. Additionally, the main features and benefits of using coupled characterization techniques are discussed in this book.

Ultra-High Performance Liquid Chromatography and Its Applications

Q. Alan Xu
Ultra High Performance Liquid Chromatography and Its Applications Book PDF

Author : Q. Alan Xu
Publisher : John Wiley & Sons
Page : 342 pages
File Size : 52,6 Mb
Release : 2013-04-01
Category : Technology & Engineering
ISBN : 9781118533987

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Ultra-High Performance Liquid Chromatography and Its Applications by Q. Alan Xu Pdf

Explores both the benefits and limitations of new UHPLC technology High performance liquid chromatography (HPLC) has been widely used in analytical chemistry and biochemistry to separate, identify, and quantify compounds for decades. The science of liquid chromatography, however, was revolutionized a few years ago with the advent of ultra-high performance liquid chromatography (UHPLC), which made it possible for researchers to analyze sample compounds with greater speed, resolution, and sensitivity. Ultra-High Performance Liquid Chromatography and Its Applications enables readers to maximize the performance of UHPLC as well as develop UHPLC methods tailored to their particular research needs. Readers familiar with HPLC methods will learn how to transfer these methods to a UHPLC platform and vice versa. In addition, the book explores a variety of UHPLC applications designed to support research in such fields as pharmaceuticals, food safety, clinical medicine, and environmental science. The book begins with discussions of UHPLC method development and method transfer between HPLC and UHPLC platforms. It then examines practical aspects of UHPLC. Next, the book covers: Coupling UHPLC with mass spectrometry Potential of shell particles in fast liquid chromatography Determination of abused drugs in human biological matrices Analyses of isoflavones and flavonoids Therapeutic protein characterization Analysis of illicit drugs The final chapter of the book explores the use of UHPLC in drug metabolism and pharmacokinetics studies for traditional Chinese medicine. With its frank discussions of UHPLC's benefits and limitations, Ultra-High Performance Liquid Chromatography and Its Applications equips analytical scientists with the skills and knowledge needed to take full advantage of this new separation technology.

HPLC

Joel K. Swadesh
HPLC Book PDF

Author : Joel K. Swadesh
Publisher : CRC Press
Page : 480 pages
File Size : 46,6 Mb
Release : 2000-12-21
Category : Science
ISBN : 9781420042665

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HPLC by Joel K. Swadesh Pdf

Product specifications, regulatory constraints, and tight production schedules impose considerable pressures on separation scientists in industry. The first edition of HPLC: Practical and Industrial Applications helped eliminate the need for extensive library or laboratory research when confronting a problem, an unfamiliar technique, or work in a new area. Its plain language, comprehensive coverage of separation topics, and practical organization made it an accessible and convenient reference manual for anyone working in or just entering the field. Since its publication in 1997, however, much has changed. The areas of mass spectroscopy, electrophoretic separations, and ultra-micro separations have blossomed, focus on quality control has intensified, and the literature has grown significantly. The Second Edition incorporates all of these changes and more. It is now fully current, with chapter supplements that include updated references and discussions of techniques. This book examines analytical HPLC as it is actually used in industry. Whether you are just entering industry, switching from one industry to another, or simply enjoy understanding how things are made, HPLC: Practical and Industrial Applications will help you solve problems and get up to speed in new areas quickly, comfortably, and with a genuine sense of mastery.

The HPLC Solvent Guide

Paul Charles Sadek
The HPLC Solvent Guide Book PDF

Author : Paul Charles Sadek
Publisher : John Wiley & Sons
Page : 670 pages
File Size : 50,5 Mb
Release : 2002-02-27
Category : Science
ISBN : UOM:39015054294031

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The HPLC Solvent Guide by Paul Charles Sadek Pdf

High-performance liquid chromatography (HPLC) is a procedure for separating components from a mixture of chemical substances; a combination of separation, identification, and quantitative measurements. Solvent selection is perhaps the most commonly overlooked parameter in HPLC. Even the most experienced analytical chemist tends to select one of three familiar solvents. The HPLC Solvent Guide provides detailed coverage of all commonly used HPLC solvents used in a wide range of separations. HPLC is a mature but substantial market, and one that Wiley reaches successfully and well. The HPLC list is established, and this second edition of a successful title will build upon the success of the first. This is a revised and expanded edition in a field that is still growing into areas of analysis and methods.