Xenopus Protocols

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Xenopus Protocols

X. Johné Liu
Xenopus Protocols Book PDF

Author : X. Johné Liu
Publisher : Springer Science & Business Media
Page : 487 pages
File Size : 44,7 Mb
Release : 2008-02-02
Category : Science
ISBN : 9781597450003

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Xenopus Protocols by X. Johné Liu Pdf

A collection of standard and cutting-edge techniques for using Xenopus oocytes and oocytes/egg extracts to reconstitute biological and cellular processes. These readily reproducible methods take advantage of the oocyte's impressive protein abundance, its striking protein translation capacity, and its breathtaking possibilities for the assembly of infectious viral particles by single cell injection of multiple RNAs. The authors focus on the versatility of frog oocytes and egg extracts in cell biology and signal transduction, and cover all the major uses of oocytes/extracts as experimental models.

Xenopus Protocols

STEFAN HOPPLER,Peter D Vize
Xenopus Protocols Book PDF

Author : STEFAN HOPPLER,Peter D Vize
Publisher : Humana Press
Page : 568 pages
File Size : 48,9 Mb
Release : 2016-08-23
Category : Medical
ISBN : 1493959530

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Xenopus Protocols by STEFAN HOPPLER,Peter D Vize Pdf

During the 20th century Xenopus has flourished as an accessible experimental model system for studying vertebrate biology. The second edition of Xenopus Protocols:Post-Genomic Approaches expands upon the previous edition with novel approaches inspired by Xenopus tropicalis and the sequencing of its genome. Xenopus tropicalis genomics and tractable genetics combined with the unique experimental advantages of Xenopus laevis and their distinctive phylogenetic position make them unique model systems for vertebrate embryonic development in the 21st century. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Xenopus Protocols: Post-Genomic Approaches, Second Edition provides a toolkit that will enable every laboratory to maximize the power of this extraordinary experimental system in the post-genomic age.

Xenopus

Kris Vleminckx
Xenopus Book PDF

Author : Kris Vleminckx
Publisher : Unknown
Page : 302 pages
File Size : 46,8 Mb
Release : 2018
Category : Electronic books
ISBN : 1493987844

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Xenopus by Kris Vleminckx Pdf

This volume explores techniques used to study and experiment with Xenopus in order to model or understand human disease. The chapters in this book cover topics such as implementation of CRISPR/Cas9 and TALEN in Xenopus from the design stage up to the genotyping stage; methods to explore generation of knock-in animals; cancer modelling and in vivo screening of congenital heart disease; electroporation; and phenotyping at the organismal level, cellular level, and proteome level. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Xenopus: Methods and Protocols is a valuable resource for researchers who are interested in expanding their knowledge of this wide and developing field. This book will aid in keeping Xenopus at the forefront for biochemical, cell biological, and developmental studies and forward it as a preclinical disease model.

Xenopus laevis: Practical Uses in Cell and Molecular Biology

Anonim
Xenopus laevis Practical Uses in Cell and Molecular Biology Book PDF

Author : Anonim
Publisher : Academic Press
Page : 646 pages
File Size : 48,6 Mb
Release : 1992-02-03
Category : Science
ISBN : 0080859348

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Xenopus laevis: Practical Uses in Cell and Molecular Biology by Anonim Pdf

This volume presents the first comprehensive treatment of the wide range of uses for Xenopus laevis oocytes and embryos in cell and molecular biology. Each chapter includes background information, experimental protocols, and suggested applications. An extensive array of techniques is featured. The authors are experienced researchers who have written chapters that will be useful to both experienced researchers and to those new to Xenopus as an experimental system. Full-color plates and diagrams enhance the educational value of this book, which provides a valuable permanent resource for all laboratories that use Xenopus. * Features approximately twenty full-color plates illustrating experimental techniques and results and depicting embryonic development * Provides complete coverage of Xenopus laevis as an experimental system including * Embryonic development, genetics, and laboratory care * Up-to-date protocols for experimental techniques using oocytes and embryos * General information listing recipes, suppliers, sequences, codons, and clones

Chromatin Protocols

Peter B. Becker
Chromatin Protocols Book PDF

Author : Peter B. Becker
Publisher : Springer Science & Business Media
Page : 525 pages
File Size : 45,5 Mb
Release : 2008-02-03
Category : Science
ISBN : 9781592596812

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Chromatin Protocols by Peter B. Becker Pdf

More than 40 years after the discovery of the nucleosome as the fun- mental unit of chromatin, the multifaceted problem of how variations in ch- matin structure affect the activity of the eukaryotic genome has not been solved. However, during the past few years research on chromatin structure and fu- tion has gained considerable momentum, and impressive progress has been made at the level of concept development as well as filling in crucial detail. The structure of the nucleosome has been visualized at unprecedented reso- tion. Powerful multisubunit enzymes have been identified that alter histone/ DNA interactions in ways that expose regulatory sequences to factors initi- ing and regulating such nuclear processes as transcription. Though the imp- tance of posttranslational modifications of histones, notably their acetylation, has long been known, the finding that a number of bona fide regulators increase transcription by acetylating nucleosomes has lent new support to the old idea that the process of gene regulation is intimately related to the nature of the chromatin environment. A wealth of nonhistone proteins contribute to a continuum of structures with distinct biochemical properties and varying degrees of DNA condensation. Perhaps the most important conclusion from a large number of studies is a fresh appreciation of the dynamic nature of chromatin structure, the built-in flexibility providing the basis for regulation.

International Review of Cytology

Anonim
International Review of Cytology Book PDF

Author : Anonim
Publisher : Academic Press
Page : 336 pages
File Size : 45,5 Mb
Release : 1996-02-12
Category : Science
ISBN : 0080857035

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International Review of Cytology by Anonim Pdf

International Review of Cytology presents current advances and comprehensive reviews in cell biology-both plant and animal. Articles address structure and control of gene expression, nucleocytoplasmic interactions, control of cell development and differentiation, and cell transformation and growth. Authored by some of the foremost scientists in the field, each volume provides up-to-date information and directions for future research.

Methods in Cell Biology

Brian K. Kay,Leslie Wilson,H. Benjamin Peng
Methods in Cell Biology Book PDF

Author : Brian K. Kay,Leslie Wilson,H. Benjamin Peng
Publisher : Academic Press
Page : 646 pages
File Size : 51,7 Mb
Release : 1991-12-17
Category : Electronic
ISBN : 0125641362

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Methods in Cell Biology by Brian K. Kay,Leslie Wilson,H. Benjamin Peng Pdf

This volume presents the first comprehensive treatment of the wide range of uses for Xenopus laevis oocytes and embryos in cell and molecular biology. Each chapter includes background information, experimental protocols, and suggested applications. An extensive array of techniques is featured. The authors are experienced researchers who have written chapters that will be useful to both experienced researchers and to those new to Xenopus as an experimental system. Full-color plates and diagrams enhance the educational value of this book, which provides a valuable permanent resource for all laboratories that use Xenopus. * Features approximately twenty full-color plates illustrating experimental techniques and results and depicting embryonic development * Provides complete coverage of Xenopus laevis as an experimental system including * Embryonic development, genetics, and laboratory care * Up-to-date protocols for experimental techniques using oocytes and embryos * General information listing recipes, suppliers, sequences, codons, and clones

Developmental Biology Protocols

Rocky S. Tuan,Cecilia W. Lo
Developmental Biology Protocols Book PDF

Author : Rocky S. Tuan,Cecilia W. Lo
Publisher : Springer Science & Business Media
Page : 537 pages
File Size : 53,8 Mb
Release : 2008-02-05
Category : Science
ISBN : 9781592590650

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Developmental Biology Protocols by Rocky S. Tuan,Cecilia W. Lo Pdf

Developmental biology is one of the most exciting and fast-growing fields today. In part, this is so because the subject matter deals with the innately fascinating biological events—changes in form, structure, and function of the org- ism. The other reason for much of the excitement in developmental biology is that the field has truly become the unifying melting pot of biology, and provides a framework that integrates anatomy, physiology, genetics, biochemistry, and cellular and mole- lar biology, as well as evolutionary biology. No longer is the study of embryonic development merely “embryology.” In fact, development biology has produced - portant paradigms for both basic and clinical biomedical sciences alike. Although modern developmental biology has its roots in “experimental emb- ology” and the even more classical “chemical embryology,” the recent explosive and remarkable advances in developmental biology are critically linked to the advent of the “cellular and molecular biology revolution.” The impressive arsenal of expe- mental and analytical tools derived from cell and molecular biology, which promise to continue to expand, together with the exponentially developing sophistication in fu- tional imaging and information technologies, guarantee that the study of the devel- ing embryo will contribute one of the most captivating areas of biological research in the next millennium.

GTPase Protocols

Ed Manser,Thomas Leung
GTPase Protocols Book PDF

Author : Ed Manser,Thomas Leung
Publisher : Springer Science & Business Media
Page : 259 pages
File Size : 55,8 Mb
Release : 2008-02-03
Category : Science
ISBN : 9781592592814

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GTPase Protocols by Ed Manser,Thomas Leung Pdf

In the last 10 years researchers have firmly established key roles for R- related GTPases in almost every aspect of cell biology. In the 1980s the pro- oncogene Ras itself was the focus of interest, though in the 1990s this shifted to the increasing variety of Ras-related proteins. In this new decade much yet needs to be done to establish the role for all the small GTPases now uncovered by the human genome project. In particular, these GTPases need to be und- stood in the appropriate biochemical and cellular contexts. In the process of trying to uncover the role of these versatile proteins, a variety of novel te- niques and methodologies has been developed. These now enable investi- tors to move easily within a diversity of fields ranging from structural studies to real-time in vivo analysis of a GTPase. In recognition of the need for access to key background methodologies, GTPase Protocols: The Ras Superfamily is devoted to techniques that are pr- ently widely used and that will continue to be the standard for researchers worldwide. Each chapter is aimed at supplying detailed methodologies to allow reproduction in any laboratory, while also providing the general pr- ciples on which the methods are based. Some of the techniques grouped in the first section apply broadly to small GTPases, whereas others in Part II are more applicable within each GTPase subfamily.

In Vitro Transcription and Translation Protocols

Martin J. Tymms
In Vitro Transcription and Translation Protocols Book PDF

Author : Martin J. Tymms
Publisher : Springer Science & Business Media
Page : 426 pages
File Size : 49,5 Mb
Release : 2008-02-02
Category : Science
ISBN : 9781592595242

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In Vitro Transcription and Translation Protocols by Martin J. Tymms Pdf

Most laboratories conducting studies that use molecular biology techniques employ in vitro transcription and translation systems as a routine part of their day-to-day research. The commercial availability of purified bacterial RNA polymerase and the availability of robust tra- lation systems has made in vitro systems attractive not only as an alt- native to the in vivo expression of genes, but also as good model systems for studying specific aspects of transcription and translation. Although fairly efficient eukaryotic translation systems have been established for a number of years, reconstitution of transcription in vitro has proved to be more difficult. Recent improvements in fractionation techniques and the cloning of proteins involved in transcription have made this a fast moving area of research. Considerable progress has also been made in recent years in developing in vitro systems to study transcription and translation in chloroplasts and mitochondria, together with systems for the study of protein import. In Vitro Transcription and Translation Protocols provides many detailed experimental procedures for prokaryotic transcription and translation systems, together with protocols for many key techniques used in the analysis of eukaryotic transcription. In keeping with the successful format of preceding volumes of the Methods in Molecular Biology series, step-by-step instructions are provided, together with extensive notes that cover troubleshooting and special tips considered important.

Nuclear Transfer Protocols

Paul J. Verma
Nuclear Transfer Protocols Book PDF

Author : Paul J. Verma
Publisher : Springer Science & Business Media
Page : 684 pages
File Size : 55,7 Mb
Release : 2008-02-02
Category : Science
ISBN : 9781597451543

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Nuclear Transfer Protocols by Paul J. Verma Pdf

Nuclear Transfer Protocols: Cell Reprogramming and Transgenesis is a comprehensive review of nuclear transfer technology in vertebrates, aimed at reprogramming differentiated nuclei and effecting targeted gene transfer. The emphasis here is on providing readily reproducible techniques for the gene- tion of cloned embryos and animals in a number of key research and commercially important vertebrates. Additional chapters provide alternative cutting-edge methods for nuclear transfer, such as zona-free nuclear transfer and serial nuclear transfer. Of immense practical benefit are descriptions of procedures associated with cloning, such as in vitro maturation of oocytes, activation and culture of cloned embryos, maintenance of pregnancy, and neonatal care of clones. Nuclear Transfer Protocols: Cell Reprogramming and Transgenesis also provides an understanding of the factors involved in nuclear reprogramming, which is imperative for the success of cloning. A section dealing with such cloning-related issues as aging and normality of clones is also included making this an essential comprehensive handbook for research and commercial labo- tories involved in, or intending to work on, nuclear transfer. The volume will prove beneficial to molecular biologists, stem cell biologists, clinicians, biotechnologists, students, veterinarians, and animal care technicians involved with reprogramming, nuclear transfer, and transgenesis.

Centrosome and Centriole

Anonim
Centrosome and Centriole Book PDF

Author : Anonim
Publisher : Academic Press
Page : 434 pages
File Size : 41,6 Mb
Release : 2015-09-10
Category : Science
ISBN : 9780128026380

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Centrosome and Centriole by Anonim Pdf

This new volume of Methods in Cell Biology looks at methods for analyzing centrosomes and centrioles. Chapters cover such topics as methods to analyze centrosomes, centriole biogenesis and function in multi-ciliated cells, laser manipulation of centrosomes or CLEM, analysis of centrosomes in human cancers and tissues, proximity interaction techniques to study centrosomes, and genome engineering for creating conditional alleles in human cells. Covers sections on model systems and functional studies, imaging-based approaches and emerging studies Chapters are written by experts in the field Cutting-edge material

Clinical Applications of PCR

Y. M. Dennis Lo
Clinical Applications of PCR Book PDF

Author : Y. M. Dennis Lo
Publisher : Springer Science & Business Media
Page : 204 pages
File Size : 48,8 Mb
Release : 2008-02-04
Category : Science
ISBN : 9781597450744

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Clinical Applications of PCR by Y. M. Dennis Lo Pdf

In this updated second edition, leading researchers apply molecular diagnostics to the many recent advances that have occurred in polymerase chain reaction( PCR)-based technologies. Highlights include real-time PCR, which allows the technique to be performed in a quantitative manner with improved sensitivity, robustness, and resilience to carryover contamination, mass spectrometric analysis of nucleic acids, and circulating cell-free nucleic acids in plasma. The authors apply these innovations to a broad spectrum of applications, including gene expression, methylation, trace molecule, gene dosage, and single cell analysis.

New and Emerging Proteomic Techniques

Dobrin Nedelkov,Randall W. Nelson
New and Emerging Proteomic Techniques Book PDF

Author : Dobrin Nedelkov,Randall W. Nelson
Publisher : Springer Science & Business Media
Page : 237 pages
File Size : 51,8 Mb
Release : 2008-02-04
Category : Science
ISBN : 9781597450263

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New and Emerging Proteomic Techniques by Dobrin Nedelkov,Randall W. Nelson Pdf

Leading researchers and innovators describe in step-by-step detail the latest techniques that promise to significantly impact the practice of proteomics, as well as its success in developing novel clinical agents. The methods span the entire spectrum of top-down and bottom-up approaches, including microarrays, gels, chromatography, and affinity separations, and address every aspect of the human proteome, both quantitatively and qualitatively. The techniques of protein detection utilized are diverse and range from fluorescence and resonance light scattering to surface plasmon resonance and mass spectrometry. The protocols follow the successful Methods in Molecular BiologyTM series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Protein Design

Raphael Guerois,Manuela López de la Paz
Protein Design Book PDF

Author : Raphael Guerois,Manuela López de la Paz
Publisher : Springer Science & Business Media
Page : 303 pages
File Size : 49,9 Mb
Release : 2008-02-04
Category : Science
ISBN : 9781597451161

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Protein Design by Raphael Guerois,Manuela López de la Paz Pdf

Protein Design: Methods and Applications presents the most up-to-date protein design and engineering strategies so that readers can undertake their own projects with a maximum chance of success. The authors present integrated computational approaches that require various degrees of computational complexity, and the major accomplishments that have been achieved in the design and structural characterization of helical peptides and proteins.