Yeast Cell Surface Engineering

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Yeast Cell Surface Engineering

Author : Mitsuyoshi Ueda
Publisher : Springer
Page : 185 pages
File Size : 43,6 Mb
Release : 2019-04-23
Category : Medical
ISBN : 9789811358685

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Yeast Cell Surface Engineering by Mitsuyoshi Ueda Pdf

This book provides a detailed and up-to-date overview of all aspects of yeast cell surface engineering, including fundamental principles, practical strategies for the construction of engineered yeasts, as well as medical and industrial applications. The technique makes it possible to add eukaryotic modifications to the surface-displayed proteins/peptides, which is of significant value in basic and applied research. Generally referred to as an arming (molecular display) technology, it allows yeast to be used as a whole-cell biocatalyst for a range of purposes, including bio-energy production, pollutant removal, recovery of rare metal ions, and preparation of functional cells, all of which are comprehensively covered in the book. Among the medical applications discussed are in vitro antibody preparation and the production of oral vaccines. In addition, it presents the latest advances in protein engineering and high-throughput screening for directed evolution of enzymes. The book enables graduate students and researchers to gain a deeper, comprehensive understanding of the technology, and offers further inspiration for researchers and industrial experts in this rapidly evolving field.

Cell Surface Engineering

Author : Rawil Fakhrullin,Insung Choi
Publisher : Royal Society of Chemistry
Page : 266 pages
File Size : 44,7 Mb
Release : 2014-07-24
Category : Medical
ISBN : 9781849739023

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Cell Surface Engineering by Rawil Fakhrullin,Insung Choi Pdf

A summary of the recent achievements in surface-functionalised cells including fabrication, characterisation, applications and nanotoxicity.

Cell Surface Engineering

Author : Rawil F Fakhrullin,Insung Choi,Yuri Lvov
Publisher : Royal Society of Chemistry
Page : 266 pages
File Size : 43,7 Mb
Release : 2014-07-08
Category : Technology & Engineering
ISBN : 9781782628477

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Cell Surface Engineering by Rawil F Fakhrullin,Insung Choi,Yuri Lvov Pdf

Cell surface engineering is an emerging field concerning cell surface modifications to enhance its functionalities. The book introduces the reader to the area of surface-functionalized cells and summarizes recent developments in the area including fabrication, characterization, applications and nanotoxicity. Topics covered include recent approaches for the functionalization of cells with nanomaterials (polymer nanofilms and nanoparticles), fabrication of functional biomimetic devices and assemblies based on nanoparticle-modified microbial cells and artificial spores (the bioinspired encapsulation of living cells with inorganic nanoshells). The book provides an interdisciplinary approach to the topic with authors from both biological and chemical backgrounds. This multidisciplinary view makes the book suitable for those interested in biomaterials, biochemistry, microbiology and colloid chemistry, providing both an introduction for postgraduate students as well as a comprehensive summary for those already working in the area. biomaterials, biochemistry, microbiology and colloid chemistry.

Yeast Metabolic Engineering

Author : Valeria Mapelli,Maurizio Bettiga
Publisher : Springer Nature
Page : 300 pages
File Size : 49,7 Mb
Release : 2022-07-04
Category : Science
ISBN : 9781071623992

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Yeast Metabolic Engineering by Valeria Mapelli,Maurizio Bettiga Pdf

This second edition volume expands on the previous edition with new and updated chapters on the latest developments in the study of yeast within the biotechnology field. The chapters in this book cover topics such as transformation protocols for genetic engineering of Saccaromyces cerevisiae and Komagataella spp.; an overview of selection markers, promoters, and strains used for metabolic engineering of S. cerevisiae, P. pastoris, and Z. bailii; the use of yeast in CRISPR/Cas9 technology; tools to study metabolic pathway in Yarrowia lypolitica; and a discussion on the “universal expression system” that is applied in a broad spectrum of fungal species. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Yeast Metabolic Engineering: Methods and Protocols, Second Edition is a valuable resource for researchers and scientists interested in learning more about this important and developing field.

Yeast Surface Display

Author : Bin Liu
Publisher : Humana
Page : 0 pages
File Size : 41,9 Mb
Release : 2015-06-11
Category : Science
ISBN : 1493927477

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Yeast Surface Display by Bin Liu Pdf

In addition to research and discovery, yeast surface display technology has found applications in industrial processes such as biofuel production and environmental pollutant absorption and degradation. Yeast Surface Display: Methods, Protocols, and Applications guides readers through yeast surface antibody display library and antibody engineering, yeast surface display as a tool for protein engineering, yeast surface cDNA display library construction and applications, and yeast surface display in bioassay and industrial applications. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Concise and easy-to-use, Yeast Surface Display: Methods, Protocols, and Applications aims to help accelerate the work of protein chemists, antibody engineers, molecular and cell biologists, and industrial bioengineers. ​

Yeast Surface Display

Author : Michael W. Traxlmayr
Publisher : Springer Nature
Page : 643 pages
File Size : 41,7 Mb
Release : 2022-04-28
Category : Science
ISBN : 9781071622858

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Yeast Surface Display by Michael W. Traxlmayr Pdf

This detailed volume explores a wide variety of applications of yeast surface display, an extensively used protein engineering technology. Beginning with detailed protocols for the construction and efficient selection/screening of yeast surface display libraries, as well as for the analysis of individual yeast-displayed protein variants, the book continues with protocols describing the selection of yeast surface display libraries for binding to mammalian cells or to extracellular matrix as well as protocols for a broad spectrum of specialized yeast surface display applications, demonstrating the versatility of this display platform. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible methodologies, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Yeast Surface Display serves as a comprehensive resource that enables the implementation of this powerful and versatile technique in virtually any molecular biology laboratory, even in the absence of any prior yeast surface display experience.

Materials Science and Engineering: Concepts, Methodologies, Tools, and Applications

Author : Management Association, Information Resources
Publisher : IGI Global
Page : 1804 pages
File Size : 44,6 Mb
Release : 2017-01-11
Category : Technology & Engineering
ISBN : 9781522517993

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Materials Science and Engineering: Concepts, Methodologies, Tools, and Applications by Management Association, Information Resources Pdf

The design and study of materials is a pivotal component to new discoveries in the various fields of science and technology. By better understanding the components and structures of materials, researchers can increase its applications across different industries. Materials Science and Engineering: Concepts, Methodologies, Tools, and Applications is a compendium of the latest academic material on investigations, technologies, and techniques pertaining to analyzing the synthesis and design of new materials. Through its broad and extensive coverage on a variety of crucial topics, such as nanomaterials, biomaterials, and relevant computational methods, this multi-volume work is an essential reference source for engineers, academics, researchers, students, professionals, and practitioners seeking innovative perspectives in the field of materials science and engineering.

Biotechnology and Bioengineering

Author : William G. Flynne
Publisher : Nova Publishers
Page : 292 pages
File Size : 41,9 Mb
Release : 2008
Category : Science
ISBN : 1604560673

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Biotechnology and Bioengineering by William G. Flynne Pdf

Biotechnology is a popular term for the generic technology of the 21st century. Although it has been utilised for centuries in traditional production processes, modern biotechnology is only 50 years old and in the last decades it has been witnessing tremendous developments. Bioengineering is the science upon which all Biotechnological applications are based. With the development of new approaches and modern techniques, traditional biotechnology industries are also acquiring new horizons enabling them to improve the quality of their products and increase the productivity of their systems. Biological engineering (also biosystems engineering and bioengineering) deals with engineering biological processes in general. It is a broad-based engineering discipline that also may involve product design, sustainability and analysis of biological systems. In other words, Bioengineering is a discipline that applies engineering principles to biological systems for the purpose of developing new technologies of services to improve the living standards of societies. It exploits the new developments in molecular biology, biochemistry, microbiology, cell metabolism and engineering principles and applies them in order to understand living systems and to bring solutions various problems associated with these systems. This book presents leading research in both areas.

Systems Metabolic Engineering

Author : Christoph Wittmann,Sang Yup Lee
Publisher : Springer Science & Business Media
Page : 391 pages
File Size : 47,5 Mb
Release : 2012-06-15
Category : Medical
ISBN : 9789400745346

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Systems Metabolic Engineering by Christoph Wittmann,Sang Yup Lee Pdf

Systems Metabolic Engineering is changing the way microbial cell factories are designed and optimized for industrial production. Integrating systems biology and biotechnology with new concepts from synthetic biology enables the global analysis and engineering of microorganisms and bioprocesses at super efficiency and versatility otherwise not accessible. Without doubt, systems metabolic engineering is a major driver towards bio-based production of chemicals, materials and fuels from renewables and thus one of the core technologies of global green growth. In this book, Christoph Wittmann and Sang-Yup Lee have assembled the world leaders on systems metabolic engineering and cover the full story – from genomes and networks via discovery and design to industrial implementation practises. This book is a comprehensive resource for students and researchers from academia and industry interested in systems metabolic engineering. It provides us with the fundaments to targeted engineering of microbial cells for sustainable bio-production and stimulates those who are interested to enter this exiting research field.

Enzymes in the Valorization of Waste

Author : Pradeep Verma
Publisher : CRC Press
Page : 279 pages
File Size : 53,7 Mb
Release : 2022-12-19
Category : Science
ISBN : 9781000683288

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Enzymes in the Valorization of Waste by Pradeep Verma Pdf

Enzymes in the Valorization of Waste: Next-Gen Technological Advances for Sustainable Development of Enzyme-based Biorefinery focusses on key enzymes which are involved in the development of integrated biorefinery. It highlights the modern next-gen technologies for promoting the application of sustainable and greener enzymatic steps at industrial scale for the development of futuristic and self-sustainable "consolidated/integrated biorefinery/enzyme-based biorefinery." It also deals with technological advancement for improvement of enzyme yield or specificity, conversion capability, such as protein and metabolic engineering and advances in next generation technologies, and so forth. Features: • Explores all modern-day technologies that can potentially be used in enzyme-based biorefinery conversion of wastes to value-added products. • Covers technological, economic, and environmental assessments of enzyme-based biorefinery prospects. • Deliberates all possible products that can be generated from wastes including biofuel and essential chemicals. • Illustrates techniques for enhanced yield and properties to be used in various industrial applications. • Reviews advanced information of relevant sources and mechanism of enzymes. This book is aimed at graduate students, researchers and related industry professionals in biochemical engineering, environmental science, wastewater treatment, biotechnology, applied microbiology, biomass-based biorefinery, biochemistry, green chemistry, sustainable development, waste treatment, enzymology, microbial biotechnology, and waste valorization.

Immobilized Biocatalysts

Author : Peter Grunwald
Publisher : MDPI
Page : 511 pages
File Size : 47,5 Mb
Release : 2018-11-14
Category : Electronic books
ISBN : 9783038973188

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Immobilized Biocatalysts by Peter Grunwald Pdf

This book is a printed edition of the Special Issue "Immobilized Biocatalysts" that was published in Catalysts

Bioprocessing of Renewable Resources to Commodity Bioproducts

Author : Virendra S. Bisaria,Akihiko Kondo
Publisher : John Wiley & Sons
Page : 640 pages
File Size : 52,5 Mb
Release : 2014-04-07
Category : Science
ISBN : 9781118845332

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Bioprocessing of Renewable Resources to Commodity Bioproducts by Virendra S. Bisaria,Akihiko Kondo Pdf

This book provides the vision of a successful biorefinery—the lignocelluloic biomass needs to be efficiently converted to its constituent monomers, comprising mainly of sugars such as glucose, xylose, mannose and arabinose. Accordingly, the first part of the book deals with aspects crucial for the pretreatment and hydrolysis of biomass to give sugars in high yield, as well as the general aspects of bioprocessing technologies which will enable the development of biorefineries through inputs of metabolic engineering, fermentation, downstream processing and formulation. The second part of the book gives the current status and future directions of the biological processes for production of ethanol (a biofuel as well as an important commodity raw material), solvents (butanol, isobutanol, butanediols, propanediols), organic acids (lactic acid, 3-hydroxy propionic acid, fumaric acid, succinic acid and adipic acid), and amino acid (glutamic acid). The commercial production of some of these commodity bioproducts in the near future will have a far reaching effect in realizing our goal of sustainable conversion of these renewable resources and realizing the concept of biorefinery. Suitable for researchers, practitioners, graduate students and consultants in biochemical/ bioprocess engineering, industrial microbiology, bioprocess technology, metabolic engineering, environmental science and energy, the book offers: Exemplifies the application of metabolic engineering approaches for development of microbial cell factories Provides a unique perspective to the industry about the scientific problems and their possible solutions in making a bioprocess work for commercial production of commodity bioproducts Discusses the processing of renewable resources, such as plant biomass, for mass production of commodity chemicals and liquid fuels to meet our ever- increasing demands Encourages sustainable green technologies for the utilization of renewable resources Offers timely solutions to help address the energy problem as non-renewable fossil oil will soon be unavailable

Enzymes and Their Biotechnological Applications

Author : Pabulo H. Rampelotto
Publisher : MDPI
Page : 467 pages
File Size : 43,5 Mb
Release : 2018-10-02
Category : Biotechnology
ISBN : 9783038421481

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Enzymes and Their Biotechnological Applications by Pabulo H. Rampelotto Pdf

This book is a printed edition of the Special Issue "Enzymes and Their Biotechnological Applications" that was published in Biomolecules

Protein Engineering

Author : Huimin Zhao
Publisher : John Wiley & Sons
Page : 41 pages
File Size : 52,5 Mb
Release : 2021-08-23
Category : Science
ISBN : 9783527344703

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Protein Engineering by Huimin Zhao Pdf

A one-stop reference that reviews protein design strategies to applications in industrial and medical biotechnology Protein Engineering: Tools and Applications is a comprehensive resource that offers a systematic and comprehensive review of the most recent advances in the field, and contains detailed information on the methodologies and strategies behind these approaches. The authors—noted experts on the topic—explore the distinctive advantages and disadvantages of the presented methodologies and strategies in a targeted and focused manner that allows for the adaptation and implementation of the strategies for new applications. The book contains information on the directed evolution, rational design, and semi-rational design of proteins and offers a review of the most recent applications in industrial and medical biotechnology. This important book: Covers technologies and methodologies used in protein engineering Includes the strategies behind the approaches, designed to help with the adaptation and implementation of these strategies for new applications Offers a comprehensive and thorough treatment of protein engineering from primary strategies to applications in industrial and medical biotechnology Presents cutting edge advances in the continuously evolving field of protein engineering Written for students and professionals of bioengineering, biotechnology, biochemistry, Protein Engineering: Tools and Applications offers an essential resource to the design strategies in protein engineering and reviews recent applications.

Metabolic Engineering

Author : Sang Yup Lee,E. Terry Papoutsakis
Publisher : CRC Press
Page : 564 pages
File Size : 51,9 Mb
Release : 1999-09-29
Category : Science
ISBN : 082477390X

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Metabolic Engineering by Sang Yup Lee,E. Terry Papoutsakis Pdf

This unique reference/text presents the basic theory and practical applications of metabolic engineering (ME). It offers systematic analysis of complex metabolic pathways and ways of employing recombinant DNA techniques to alter cell behavior, metabolic patterns, and product formation. Treating ME as a distinct subfield of genetic engineering, the book demonstrates new means of enabling cells to produce valuable proteins, polypeptides, and primary and secondary metabolites. Written by more than 35 leading international experts in the field, this book discusses metabolic engineering in plant and mammalian cells, bacteria, and yeasts and assesses metabolic engineering applications in agriculture, pharmaceuticals, and environmental systems. It illuminates the potential of the "cell factory" model for production of chemicals and therapeutics and examines methods for developing new antiviral and antibacterial molecules and effective gene and somatic-cell therapies. Metabolic Engineering also addresses the use of metabolic flux analysis, metabolic control analysis, and online metabolic flux analysis.