Plant Transformation Technologies

Author : Charles Neal Stewart,Alisher Touraev,Vitaly Citovsky,Tzvi Tzfira
Publisher : John Wiley & Sons
Page : 314 pages
File Size : 41,9 Mb
Release : 2011-01-31
Category : Science
ISBN : 9780470958940

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Plant Transformation Technologies by Charles Neal Stewart,Alisher Touraev,Vitaly Citovsky,Tzvi Tzfira Pdf

Plant Transformation Technologies is a comprehensive, authoritative book focusing on cutting-edge plant biotechnologies, offering in-depth, forward-looking information on methods for controlled and accurate genetic engineering. In response to ever-increasing pressure for precise and efficient integration of transgenes in plants, many new technologies have been developed. With complete coverage of these technologies, Plant Transformation Technologies provides valuable insight on current and future plant transformation technologies. With twenty-five chapters written by international experts on transformation technologies, the book includes new information on Agrobacterium, targeting transgenes into plant genomes, and new vectors and market systems. Including both review chapters and protocols for transformation, Plant Transformation Technologies is vitally important to graduate students, postdoctoral students, and university and industry researchers.

Author : Anonim
Publisher : Unknown
Page : 280 pages
File Size : 55,7 Mb
Release : 2010
Category : Electronic
ISBN : 1444392158

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Plant Transformation Technologies by Anonim Pdf

Author : Yinghui Dan,David W. Ow
Publisher : Bentham Science Publishers
Page : 154 pages
File Size : 42,8 Mb
Release : 2011
Category : Science
ISBN : 9781608052486

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Historical Technology Developments by Yinghui Dan,David W. Ow Pdf

"Plant transformation technology has played a critical role in advancing biotechnology and fundamental research and evolved as a science. This book describes the breakthrough technologies in all aspects of plant transformation in the last 27 years, which "

Author : Wagdy Sawahel
Publisher : Daya Books
Page : 146 pages
File Size : 47,7 Mb
Release : 1997
Category : Plant genetic transformation
ISBN : 8170351758

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Plant Genetic Transformation Technology by Wagdy Sawahel Pdf

The introduction of foreign genetic material into host cells is a vital step in genetic engineering. It is especially important when one considers the potential application of gene transfer systems to crop improvement with the aim of engineering specific traits into a wide variety of plants. The book is an overview of the current research into gene transfer technology and will be valuable for those, who are involved in the field of plant molecular biology, genetics, biochemistry, physiology and biotechnology. Contents Chapter 1: Genetic Transformation; History & Definition, Gene transfer systems, Natural transformation system (vector system), Direct gene transfer (vector-free systems), Genetic transformation strategy, Biological parameters, Requirements for genetic transformation, Arrangement of foreign DNA in the plant genome, Stability of the foreign gene, Modes of genetic recombination, Genetic transformation approaches, Classes of transformants, Inter-transformant variability; Chapter 2: Gene Delivery systems; Polycation-mediated transformation, Particle gun, Electroporation, Microinjection, U V laser microbeam, Electroinjection, Electrophoresis, Protoplast fusion, Macroinjection, Liposome system, Ca-DNA co-precipitation method, Silicon carbide fiber-vortex, Sonication; Chapter 3: Strategies for Improving Transformation Efficiency; Plasmid DNA, Carrier DNA, DNA repair, Transformation of synchronized protoplasts, Restriction-enzyme mediated event, Transformation booster sequence; Chapter 4: Organelle Transformation; Chapter 5: Shotgun Transformation; Plasmid rescue, Gene rescue, Promoter & enhancer rescue.

Author : Bentham Science Publishers
Publisher : Unknown
Page : 128 pages
File Size : 42,7 Mb
Release : 2011-09-10
Category : Electronic
ISBN : 1608055329

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Historical Technology Developments in Plant Transformation by Bentham Science Publishers Pdf

"Plant transformation technology has played a critical role in advancing biotechnology and fundamental research and evolved as a science. This book describes the breakthrough technologies in all aspects of plant transformation in the last 27 years, which "

Author : National Academies of Sciences, Engineering, and Medicine,Division on Earth and Life Studies,Board on Agriculture and Natural Resources,Committee on Genetically Engineered Crops: Past Experience and Future Prospects
Publisher : National Academies Press
Page : 607 pages
File Size : 42,5 Mb
Release : 2017-01-28
Category : Science
ISBN : 9780309437387

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Genetically Engineered Crops by National Academies of Sciences, Engineering, and Medicine,Division on Earth and Life Studies,Board on Agriculture and Natural Resources,Committee on Genetically Engineered Crops: Past Experience and Future Prospects Pdf

Genetically engineered (GE) crops were first introduced commercially in the 1990s. After two decades of production, some groups and individuals remain critical of the technology based on their concerns about possible adverse effects on human health, the environment, and ethical considerations. At the same time, others are concerned that the technology is not reaching its potential to improve human health and the environment because of stringent regulations and reduced public funding to develop products offering more benefits to society. While the debate about these and other questions related to the genetic engineering techniques of the first 20 years goes on, emerging genetic-engineering technologies are adding new complexities to the conversation. Genetically Engineered Crops builds on previous related Academies reports published between 1987 and 2010 by undertaking a retrospective examination of the purported positive and adverse effects of GE crops and to anticipate what emerging genetic-engineering technologies hold for the future. This report indicates where there are uncertainties about the economic, agronomic, health, safety, or other impacts of GE crops and food, and makes recommendations to fill gaps in safety assessments, increase regulatory clarity, and improve innovations in and access to GE technology.

Author : National Research Council,Institute of Medicine,Board on Agriculture and Natural Resources,Food and Nutrition Board,Board on Life Sciences,Committee on Identifying and Assessing Unintended Effects of Genetically Engineered Foods on Human Health
Publisher : National Academies Press
Page : 254 pages
File Size : 46,9 Mb
Release : 2004-07-08
Category : Science
ISBN : 9780309166157

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Safety of Genetically Engineered Foods by National Research Council,Institute of Medicine,Board on Agriculture and Natural Resources,Food and Nutrition Board,Board on Life Sciences,Committee on Identifying and Assessing Unintended Effects of Genetically Engineered Foods on Human Health Pdf

Assists policymakers in evaluating the appropriate scientific methods for detecting unintended changes in food and assessing the potential for adverse health effects from genetically modified products. In this book, the committee recommended that greater scrutiny should be given to foods containing new compounds or unusual amounts of naturally occurring substances, regardless of the method used to create them. The book offers a framework to guide federal agencies in selecting the route of safety assessment. It identifies and recommends several pre- and post-market approaches to guide the assessment of unintended compositional changes that could result from genetically modified foods and research avenues to fill the knowledge gaps.

Author : Phetole Mangena
Publisher : CRC Press
Page : 244 pages
File Size : 45,6 Mb
Release : 2022-09-01
Category : Science
ISBN : 9781000638158

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Plant Transformation via Agrobacterium Tumefaciens by Phetole Mangena Pdf

Plant Transformation via Agrobacterium Tumefaciens compiles fundamental and specific information and procedures involving in vitro soybean transformation, which forms the basis for the Agrobacterium-mediated genetic manipulation of soybean using plant tissue culture. This method serves as one of the most preferred, reliable and cost-effective mechanism of transgene expression in both leguminous recalcitrant species and non-legume crops. The technology is favoured due to its simplicity, feasibility and high transformation rates that are so far achieved mostly in monocot plants and a few dicot genotypes. This book provides a comprehensive review of plant transformation which remains necessary for many researchers who are still facing protocol-related hurdles. Among some of the major topics covered in Plant Transformation via Agrobacterium Tumefaciens are the history and discovery of Agrobacterium bacterium, longstanding challenges causing transformation inefficiencies, types and conditions of explants, development of transgenic plants for stress resistance, and the role of transgenic plants on animal/human health, including the environment. Plant Transformation via Agrobacterium Tumefaciens helps the reader to understand how soybean, like many other orphan legume crops, faces the risk of overexploitation which may render the currently available varieties redundant and extinct should its narrow gene pool not improve. Plant transformation serves as a key technique in improving the gene pool, while developing varieties that are drought tolerant, have enhanced nutritional value, pest resistant and reduce the destruction by disease causing microorganims. This book is an essential foundation tool that is available for researchers and students to reinforce the application of Agrobacterium-mediated genetic transformation in soybean.

Author : Horacio Esteban Hopp,Luis Herrera-Estrella,German Spangenberg
Publisher : Frontiers Media SA
Page : 275 pages
File Size : 55,9 Mb
Release : 2022-05-10
Category : Science
ISBN : 9782889761234

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Plant Transformation by Horacio Esteban Hopp,Luis Herrera-Estrella,German Spangenberg Pdf

Author : Leandro Peña
Publisher : Springer Science & Business Media
Page : 427 pages
File Size : 40,7 Mb
Release : 2008-02-05
Category : Science
ISBN : 9781592598274

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Transgenic Plants by Leandro Peña Pdf

The aim of Transgenic Plants: Methods and Protocols is to provide a source of information to guide the reader through a wide range of frequently used, broadly applicable, and easily reproducible techniques involved in the gene- tion of transgenic plants. Its step-by-step approach covers a series of methods for genetically transforming plant cells and tissues, and for recovering whole transgenic plants from them. The volume then moves on to the use of sele- able and reporter markers, positive selection, marker elimination after rec- ery of transgenic plants, and the analysis of transgene integration, expression, and localization in the plant genome. Although contributors usually refer to model plants in most chapters, the protocols described herein should be widely applicable to many plant species. The last two sections are devoted to me- ods of risk assessment and to exploring the current and future applications of transgenic technology in agriculture and its social implications in a case study. Transgenic Plants: Methods and Protocols is divided into six major s- tions plus an introduction, comprising 27 chapters. Part I, the Introduction, is a review of the past, present, and perspectives of the transgenic plants, from the discovery of Agrobacterium tumefaciens as a feasible transformation vector, to its use as a tool to study gene expression and function, and the current and possible future applications of this technology in agriculture, industry, and medicine.

Author : Usha Kiran,Malik Zainul Abdin,Kamaluddin
Publisher : Academic Press
Page : 345 pages
File Size : 49,6 Mb
Release : 2020-06-19
Category : Science
ISBN : 9780128186336

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Transgenic Technology Based Value Addition in Plant Biotechnology by Usha Kiran,Malik Zainul Abdin,Kamaluddin Pdf

Transgenic Technology Based Value Addition in Plant Biotechnology discusses the principles, methodology and applications of transgenic technologies. With step-by-step methods on genome editing techniques and a range of potential applications, from improving crop yield to increasing therapeutic efficacy, this book is a one-stop reference for plant gene editing technologies. It will be of particular interest to researchers interested in plant biotechnology and plant genetics, as well as agricultural scientists and those concerned with medicinal plants. Includes step-by-step methods to assist students and researchers with genome editing and bioinformatics tools Highlights a number of applications of plant biotechnology, including how to achieve desired traits, such as improved crop yield Discusses principles, methodology and applications of transgenic technologies

Author : Hee-Jong Koh,Suk-Yoon Kwon,Michael Thomson
Publisher : Springer
Page : 352 pages
File Size : 40,8 Mb
Release : 2015-08-26
Category : Science
ISBN : 9789401799966

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Current Technologies in Plant Molecular Breeding by Hee-Jong Koh,Suk-Yoon Kwon,Michael Thomson Pdf

Recent progress in biotechnology and genomics has expanded the plant breeders’ horizon providing a molecular platform on the traditional plant breeding, which is now known as ‘plant molecular breeding’. Although diverse technologies for molecular breeding have been developed and applied individually for plant genetic improvement, common use in routine breeding programs seems to be limited probably due to the complexity and incomplete understanding of the technologies. This book is intended to provide a guide for researchers or graduate students involved in plant molecular breeding by describing principles and application of recently developed technologies with actual case studies for practical use. The nine topics covered in this book include the basics on genetic analysis of agronomic traits, methods of detecting QTLs, the application of molecular markers, genomics-assisted breeding including epigenomic issues, and genome-wide association studies. Identification methods of mutagenized plants, actual case studies for the isolation and functional studies of genes, the basics of gene transfer in major crops and the procedures for commercialization of GM crops are also described. This book would be a valuable reference for plant molecular breeders and a cornerstone for the development of new technologies in plant molecular breeding for the future.

Author : Hau-Hsuan Hwang,Stanton B. Gelvin
Publisher : Frontiers Media SA
Page : 167 pages
File Size : 48,6 Mb
Release : 2015-06-26
Category : Botany
ISBN : 9782889195749

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Agrobacterium biology and its application to transgenic plant production by Hau-Hsuan Hwang,Stanton B. Gelvin Pdf

The broad host range pathogenic bacterium Agrobacterium tumefaciens has been widely studied as a model system to understand horizontal gene flow, secretion of effector proteins into host cells, and plant-pathogen interactions. Agrobacterium-mediated plant transformation also is the major method for generating transgenic plants for research and biotechnology purposes. Agrobacterium species have the natural ability to conduct interkingdom genetic transfer from bacteria to eukaryotes, including most plant species, yeast, fungi, and even animal cells. In nature, A. tumefaciens causes crown gall disease resulting from expression in plants of auxin and cytokinin biosynthesis genes encoded by the transferred (T-) DNA. Gene transfer from A. tumefaciens to host cells requires virulence (vir) genes that reside on the resident tumor-inducing (Ti) plasmid. In addition to T-DNA, several Virulence (Vir) effector proteins are also translocated to host cells through a bacterial type IV secretion system. These proteins aid in T-DNA trafficking through the host cell cytoplasm, nuclear targeting, and T-DNA integration. Genes within native T-DNAs can be replaced by any gene of interest, making Agrobacterium species important tools for plant research and genetic engineering. In this research topic, we provided updated information on several important areas of Agrobacterium biology and its use for biotechnology purposes.

Author : Kasi Azhakanandam,Aron Silverstone,Henry Daniell,Michael R. Davey
Publisher : Springer
Page : 455 pages
File Size : 52,5 Mb
Release : 2015-03-03
Category : Science
ISBN : 9781493922024

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Recent Advancements in Gene Expression and Enabling Technologies in Crop Plants by Kasi Azhakanandam,Aron Silverstone,Henry Daniell,Michael R. Davey Pdf

In this book, authors who are experts in their fields describe current advances on commercial crops and key enabling technologies that will underpin future advances in biotechnology. They discuss state of the art discoveries as well as future challenges. Tremendous progress has been made in introducing novel genes and traits into plant genomes since the first creation of transgenic plants thirty years ago, and the first commercialization of genetically modified maize in 1996. Consequently, cultivation of biotech crops with useful traits has increased more than 100-fold from 1.7 million hectares in 1996 to over 175 million hectares globally in 2013. This achievement has been made possible by continued advances in understanding the basic molecular biology of regulatory sequences to modulate gene expression, enhancement of protein synthesis and new technologies for transformation of crop plants. This book has three sections that encompass knowledge on genetically modified (GM) food crops that are currently used by consumers, those that are anticipated to reach the market place in the near future and enabling technologies that will facilitate the development of next generation GM crops. Section I focuses only on genetically modified maize and soybean (3 chapters each), while Section II discusses the GM food crops rice, wheat, sorghum, vegetables and sugar cane. Section III covers exciting recent developments in several novel enabling technologies, including gene targeting, minichromosomes, and in planta transient expression systems.

Author : Balram Sharma
Publisher : Daya Books
Page : 178 pages
File Size : 47,8 Mb
Release : 2008
Category : Horticultural crops
ISBN : 8170355273

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Plant Tissue Culture and Transformation Techniques by Balram Sharma Pdf

This publication deals with various aspects of the genetic engineering-plant tissue culture and transformation techniques. Due to their biological, ecological and geographic diversity, the demand for various horticultural crops is likely to increase manifold in the future and in order to meet such demand, there is an urgent need to concentrate on the research aspects for improvement of these crops. Plant tissues culture offers new tools to accomplish this objective. Plant tissue culture is an important area of biotechnology, whic is used for the propagation of problem-species, rapid propagation of high value genotypes, production of secondary metabolites etc. Tissue culture is an important step in developing new hybrids from distant parents and transgenics and particularly cost-effective technology with palpable impact in vegetatively propagated plants, which is celarly visible in improved yields of cultivars incorporating genes from unexplored sources and improved germplasm, enhancement of quality parameters and supply of disease-free clones of true-to-type planting materials. Plant tissue culture is the most rapid and efficacious way to speedy productin of large volumes of identical plants for specific markets. Micropropagation is the quickest way for popularization of new varieties of horiticultural crops where other methods of mass multiplication of genetically pure and homogeneous planting materials are very slow. With the advent of transformation technology, it has become a useful tool to mass produce new plants with genetic material transferred from unrelated sources with the help of tissue culture. The volume contains contributions by several authors highlighting the status of genetic engineering and plant tissue culture research and development programmes in various developing countries and case studies on a few economically important crops. The publication will be of immense value to the working scientists, institutions, policy makers and all those bearing responsibility to develop, implement and intensify programmes in the related subjects in their respective countries. This book provides a good picture of efforts being made and success already achieved in the Third World countries at various levels of development striving to secure gains from the latest advances in science and technology. Contents Chapter 1: China-Cotton Genetic Engineering and Tissue Culture Developments by Reddy Naganagouda and Zhu Shuijin; Chapter 2: Egypt: Development of Transgenic Wheat with Improved Salt and Drought Tolerance by Ahmed Bahelidin & Hala F Eissa; Chapter 3: Egypt-Use of Genetic Engineering Approach to Develop Virus Resistance for Some Plants Belonging to Different Plant Families by Atef Shoukry Sadik; Chapter 4: Egypt-Genetic Transformation of Maize (Zea mays L) by Shireen Assem; Chapter 5: Egypt-Tissue Culture and Transformation of Potato by Taymour Nasr El Din; Chapter 6: Eritrea-Genetic Engineering by Tadesse Mehari; Chapter 7: India-Present Status, Policy and Constrains in Genetic Engineering by Jeetendra Jaysing Solanki; Chapter 8: Indonesia-Review on the Role of Biotechnology for Food Security by Lukit Devy; Chapter 9: Iran-Status of Agricultural Biotechnology by M Kafi; Chapter 10: Kenya-Status of Biotechnology Research and Development by C N Ngaman, M G Karembu and D Otunge; Chapter 11: Kenya-Present Status, Policies and Constraints in Areas Related to Plant Biotechnology by Salome Mallowa Obura; Chapter 12: Malaysia-A Brief Report on Biotechnology and Genetic Engineering by Z A Aziz; Chapter 13: Pakistan-Present Status, Policies and Constraints of Biotechnology by Saghir Ahmed Sheikh; Chapter 14: Sri Lanks-Present Status of Biotechnology by P Aruni Weerasinghe; Chapter 15: Syria-Current Status and Future Prospective of Agricultural Biotechnology Program at GCSAR by Nabila Ali Bacha; Chapter 16: Uganda-Report on the Present Status Policies and Constraints to Genetic Engineering by Kyeyune Gerald Muwanga.