Biophysical Characterization Of Functional Peptides

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Biophysical Characterization of Functional Peptides

Vibin Ramakrishnan

Author : Vibin Ramakrishnan
Publisher : Springer Nature
Page : 142 pages
File Size : 42,7 Mb
Release : 2023-08-02
Category : Science
ISBN : 9781071634059

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Biophysical Characterization of Functional Peptides by Vibin Ramakrishnan Pdf

This protocol book presents reproducible and step-by-step procedures for the peptide synthesis, their characterization and applications. The volume includes an introductory section on in silico modelling of new peptide molecules, Molecular Dynamics Simulations, Docking, Electrostatic fingerprinting of peptides, and other modelling tools for peptide designing and optimization. Further, it covers protocols for the solid phase peptide synthesis, chromatographic and mass spectrometric characterization of peptides. Importantly, it covers methods for biophysical characterizations of peptides for their potential applications as drug delivery vehicles, peptide nano-assembly, bionanocatalysis, protein aggregation diseases, and peptide-based anti-bacterial.

The Peptides Analysis, Synthesis, Biology

Erhard Gross

Author : Erhard Gross
Publisher : Elsevier
Page : 330 pages
File Size : 42,9 Mb
Release : 2012-12-02
Category : Science
ISBN : 9780323155632

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The Peptides Analysis, Synthesis, Biology by Erhard Gross Pdf

The Peptides: Analysis, Synthesis, Biology: Volume 4: Modern Techniques of Conformational, Structural, and Configurational Analysis is an open-ended treatise that provides comprehensive and critical reviews of important developments in all areas of peptide research including analysis, synthesis, and biology. X-ray structure studies, amino acid analysis, and chiroptical analysis of configuration are discussed, along with solid-phase sequencing and ultramicroanalysis with the aid of fluorescence. This volume is comprised of six chapters and begins with an account of crystal structure analysis on molecules containing 2-12 peptide units, focusing on the variety of intramolecular hydrogen bonds, cis peptide units, and multiple conformation. Conformational changes upon complexation with metal ions are considered, together with the inclusion of solvents as integral parts of a molecular structure. The following chapters explore the conformations of insulin, glucagon, pancreatic polypeptide and related molecules, as well as the molecular biology of these hormones based on crystal structures; the usefulness of chiroptical techniques for determining the absolute configuration of amino acids and small peptides; and ultramicroanalysis of peptides and proteins by high performance liquid chromatography and fluorescence detection. The final chapter looks at the status and future potential of solid-phase sequencing. This book is intended as a reference for specialists, a guide for the novice, and a forum for investigators concerned with research on peptides and proteins.

Biophysical Characterization of Proteins in Developing Biopharmaceuticals

Damian J. Houde,Steven A. Berkowitz

Author : Damian J. Houde,Steven A. Berkowitz
Publisher : Elsevier
Page : 586 pages
File Size : 55,9 Mb
Release : 2019-11-13
Category : Medical
ISBN : 9780444641748

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Biophysical Characterization of Proteins in Developing Biopharmaceuticals by Damian J. Houde,Steven A. Berkowitz Pdf

Biophysical Characterization of Proteins in Developing Biopharmaceuticals, Second Edition, presents the latest on the analysis and characterization of the higher-order structure (HOS) or conformation of protein based drugs. Starting from the very basics of protein structure, this book explains the best way to achieve this goal using key methods commonly employed in the biopharmaceutical industry. This book will help today’s industrial scientists plan a career in this industry and successfully implement these biophysical methodologies. This updated edition has been fully revised, with new chapters focusing on the use of chromatography and electrophoresis and the biophysical characterization of very large biopharmaceuticals. In addition, best practices of applying statistical analysis to biophysical characterization data is included, along with practical issues associated with the concept of a biopharmaceutical’s developability and the technical decision-making process needed when dealing with biophysical characterization data. Presents basic protein characterization methods and tools applicable to (bio)pharmaceutical research and development Highlights the capabilities and limitations of each technique Discusses the underlining science of each tool Empowers industrial biophysical chemists by providing a roadmap for applying biophysical tools Outlines the needs for new characterization and analytical tools in the biopharmaceutical industry

Major Methods of Peptide Bond Formation

Erhard Gross,Johannes Meienhofer

Author : Erhard Gross,Johannes Meienhofer
Publisher : Elsevier
Page : 454 pages
File Size : 51,6 Mb
Release : 2014-05-10
Category : Science
ISBN : 9781483217963

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Major Methods of Peptide Bond Formation by Erhard Gross,Johannes Meienhofer Pdf

The Peptides: Analysis, Synthesis, Biology, Volume 1: Major Methods of Peptide Bond Formation provides comprehensive and critical reviews of important developments in three major areas of peptide research—analysis, synthesis, and biology. This book discusses the nature of the peptide bond, coupling between amino acid residues, and catalysis of active ester reactions. The formation of hydrazides, reactions of carbodiimides with amines, and mixed carbonic anhydride method of peptide synthesis are also elaborated. This publication likewise covers the control of racemization during peptide synthesis, strategies for minimizing racemization during amide-forming steps, and assays for chiral purity. This volume is suitable for students, specialists, and scientists from a broad range of disciplines concerned with peptides.

Collagen Mimetic Peptides and Their Biophysical Characterization

Jianxi Xiao

Author : Jianxi Xiao
Publisher : Springer
Page : 0 pages
File Size : 54,5 Mb
Release : 2024-09-21
Category : Science
ISBN : 9811019401

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Collagen Mimetic Peptides and Their Biophysical Characterization by Jianxi Xiao Pdf

This book embarks on an enlightening journey through the molecular landscape of collagen mimic peptides, shedding light on their significance and potential. In the intricate realm of biomolecules, collagen's preeminence as the most abundant protein in the human body, forming the foundational scaffolding of tissues and organs, renders it a subject of profound scientific interest. It explores diverse facets related to collagen mimic peptides, spanning aspects such as bioinformatic analysis, synthetic strategies, pathological collagen targeting, and the construction of homotrimeric, heterotrimeric and self-assembled peptide models. Furthermore, it provides an exhaustive investigation into biophysical techniques, encompassing Circular Dichroism (CD), X-ray crystallography, Nuclear Magnetic Resonance (NMR), Fluorescence, and Raman spectroscopy, thereby empowering researchers to unravel the structural intricacies of these peptides. The book unravels the profound implications of collagen mimic peptides across a spectrum of scientific domains, including protein science, biomaterials, bioanalysis, and beyond. Its accessibility and insights cater not only to seasoned researchers but also to undergraduate and graduate students eager to delve into the complexities of this fundamental protein.

Polypeptide Materials

Maxim G. Ryadnov

Author : Maxim G. Ryadnov
Publisher : Humana
Page : 268 pages
File Size : 43,6 Mb
Release : 2020-08-28
Category : Science
ISBN : 1071609270

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Polypeptide Materials by Maxim G. Ryadnov Pdf

This book details the synthesis and assembly of polypeptide materials across length scales, i.e. proteins and peptides, their precursors, conjugates, and derivatives. A particular emphasis is made on measurement tools and procedures for material characterization, both physicochemical and functional. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Polypeptide Materials: Methods and Protocols serves to reflect the inter-disciplinary nature of molecular biology as well as the importance of developing innovative measurement methods to advance this vital area of research.

Peptides

Bernd Gutte

Author : Bernd Gutte
Publisher : Elsevier
Page : 527 pages
File Size : 48,7 Mb
Release : 1995-10-24
Category : Science
ISBN : 9780080538594

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Peptides by Bernd Gutte Pdf

In recent years, research has shown the importance of peptides in neuroscience, immunology, and cell biology. Active research programs worldwide are now engaged in developing peptide-based drugs and vaccines using modification of natural peptides and proteins, design of artificial peptides and peptide mimetics, and screening of peptide and phage libraries. In this comprehensive book, the authors discuss peptide synthesis and application within the context of their increasing importance to the pharmaceutical industry. Peptides: Synthesis, Structures, and Applications explores the broad growth of information in modern peptide synthetic methods and the structure-activity relationships of synthetic polypeptides. The history of peptide chemistry Amide formation, deprotection, and disulfide formation in peptide synthesis Solid-phase peptide synthesis a-helix formation by peptides in water Stability and dynamics of peptide conformation An overview of structure-function studies of peptide hormones Neuropeptides:peptide and nonpeptide analogs Reversible inhibitors of serine proteinases Design of polypeptides Current capabilities and future possibilities of soluble chemical combinatorial libraries Epitope mapping with peptides Synthesis and applications of branched peptides in immunological methods and vaccines

Protein Structure Analysis

Roza Maria Kamp,Theodora Choli-Papadopoulou,Brigitte Wittmann-Liebold

Author : Roza Maria Kamp,Theodora Choli-Papadopoulou,Brigitte Wittmann-Liebold
Publisher : Springer Science & Business Media
Page : 340 pages
File Size : 51,8 Mb
Release : 1997
Category : Science
ISBN : 3540615008

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Protein Structure Analysis by Roza Maria Kamp,Theodora Choli-Papadopoulou,Brigitte Wittmann-Liebold Pdf

"Protein Structure Analysis - Preparation and Characterization" is a compilation of practical approaches to the structural analysis of proteins and peptides. Here, about 20 authors describe and comment on techniques for sensitive protein purification and analysis. These methods are used worldwide in biochemical and biotechnical research currently being carried out in pharmaceu tical and biomedical laboratories or protein sequencing facilities. The chapters have been written by scientists with extensive ex perience in these fields, and the practical parts are well documen ted so that the reader should be able to easily reproduce the described techniques. The methods compiled in this book were demonstrated in student courses and in the EMBO Practical Course on "Microsequence Analysis of Proteins" held in Berlin September 10-15, 1995. The topics also derived from a FEBS Workshop, held in Halkidiki, Thessaloniki, Greece, in April, 1995. Most of the authors participated in these courses as lecturers and tutors and made these courses extremely lively and successful. Since polypeptides greatly vary depending on their specific structure and function, strategies for their structural analysis must for the most part be adapted to each individual protein. Therefore, advantages and limitations of the experimen tal approaches are discussed here critically, so that the reader becomes familiar with problems that might be encountered.

Physical Biology of Proteins and Peptides

Luis Olivares-Quiroz,Orlando Guzmán-López,Hector Eduardo Jardón-Valadez

Author : Luis Olivares-Quiroz,Orlando Guzmán-López,Hector Eduardo Jardón-Valadez
Publisher : Springer
Page : 177 pages
File Size : 48,6 Mb
Release : 2015-10-22
Category : Science
ISBN : 9783319216874

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Physical Biology of Proteins and Peptides by Luis Olivares-Quiroz,Orlando Guzmán-López,Hector Eduardo Jardón-Valadez Pdf

This book covers the latest developments in the physical biology of proteins and peptides. Key insights into microscopic and macroscopic approaches to describe biologically relevant macromolecules and their interactions are provided. This book also covers a wide range of tools, including theoretical methods as statistical mechanics, normal mode analysis, kinetic theory and stochastic processes, and all-atom and coarse-grained molecular dynamics simulations. New experimental techniques are also discussed, particularly related to amiloidogenic peptides and their mutations. This is an excellent book for molecular biologists, physicists, computational scientists, and chemists. It covers cutting-edge research in this exciting, interdisciplinary research field. This book also: Discusses the latest developments in the physical biology of proteins, peptides and enzymes covering theoretical, computational, and experimental approaches Broadens readers’ understanding on the r ole of intra- and inter-molecular interactions as a fundamental cornerstone of macroscopic biological properties of macromolecules Provides a wide and useful perspective on different aspects of the physics, biology, and chemistry of proteins and peptides suitable for interdisciplinary research.

Peptides Frontiers of Peptide Science

James P. Tam,Pravin T.P. Kaumaya

Author : James P. Tam,Pravin T.P. Kaumaya
Publisher : Springer Science & Business Media
Page : 913 pages
File Size : 46,5 Mb
Release : 1999-02-28
Category : Medical
ISBN : 9780792351603

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Peptides Frontiers of Peptide Science by James P. Tam,Pravin T.P. Kaumaya Pdf

This volume contains the Proceedings of the Fifteenth American Peptide Symposium (15APS) held in Nashville, Tennessee, on June 14-19, 1997. This biennial meeting was jointly sponsored by the American Peptide Society and Vanderbilt University. The American Peptide Symposium is the most important international peptide meeting, and attracts the largest number of attendees and attention. The attendance of 1081 participants from 37 countries was lower than the two previous Symposia. However, the number of participating countries was the largest. Thus, it was gratifying to see that this meeting retained both its international flavor and participant loyalty at a time when there are many more symposia held each year on similar subjects. The scientific program comprised 124 oral and 550 poster presentations. The oral presentations together with selected poster presentations are included in this proceedings volume. Together they constitute the most up-to-date advances in peptide science.

Solid-Phase Peptide Synthesis

Gregg B. Fields

Author : Gregg B. Fields
Publisher : Academic Press
Page : 828 pages
File Size : 41,8 Mb
Release : 1997-10-21
Category : Medical
ISBN : UVA:X004150724

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Solid-Phase Peptide Synthesis by Gregg B. Fields Pdf

The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volumehas been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. More than 275 volumes have been published (all of them still in print) and much of the material is relevant even today-truly an essential publication for researchers in all fields of life sciences. Key Features * Solid-phase peptide synthesis * Applications of peptides for structural and biological studies * Characterization of synthetic peptides

Ion Channels: Channel Chemical Biology, Engineering, and Physiological Function

Anonim

Author : Anonim
Publisher : Academic Press
Page : 454 pages
File Size : 46,6 Mb
Release : 2021-06-12
Category : Science
ISBN : 9780128239254

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Ion Channels: Channel Chemical Biology, Engineering, and Physiological Function by Anonim Pdf

Ion Channels, Part C, Volume 653 in the Methods in Enzymology series, highlights new advances in the field with this new volume presenting interesting chapters on a variety of topics, including Nonsense suppression in ion channels, Engineering Ion Channels Using Protein Trans-splicing, Probing Ion Channel Neighborhoods Using APEX, STX based probes for NaVs, ANAP: a versatile, fluorescent probe of ion channel gating and regulation, High Throughput Screens for Small Molecule Ion Channel Modulators, Using toxins to study ion channels, Re/de-constructing ubiquitin regulation of ion channels, Tethered Peptide Toxins for Ion Channels, Voltage-Sensing Phosphatase Molecular Engineering, and more. Additional chapters cover Engineering excitable cells, Stretch and Poke Stimulation of Mechanically-Activated Ion Channels, Optical Control of STIM Channels, High Throughput Electrophysiological Evaluation of Mutant Ion Channels, Evaluating BEST1 Mutations in RPE Stem Cells, Long Read Transcript Profiling of Ion Channel Splice Variants, Permeation of Connexin Channels, Ratiometric pH indicator for melanosomes and lysosomes, and Ion channels in the epithelial cells of the choroid plexus. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in the Methods in Enzymology series

Characterization of Proteins

Felix Franks

Author : Felix Franks
Publisher : Springer
Page : 570 pages
File Size : 43,7 Mb
Release : 2007-10-03
Category : Science
ISBN : 9781592594375

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Characterization of Proteins by Felix Franks Pdf

Proteins are the servants of life. They occur in all component parts of living organisms and are staggering in their functional var- ty, despite their chemical similarity. Even the simplest single-cell organism contains a thousand different proteins, fulfilling a wide range of life-supporting roles. Their production is controlled by the cell’s genetic machinery, and a malfunction of even one protein in the cell will give rise to pathological symptoms. Additions to the total number of known proteins are constantly being made on an increasing scale through the discovery of mutant strains or their production by genetic manipulation; this latter technology has become known as protein engineering. The in vivo functioning of proteins depends critically on the chemical structure of individual peptide chains, but also on the detailed folding of the chains themselves and on their assembly into larger supramolecular structures. The molecules and their fu- tional assemblies possess a limited in vitro stability. Special methods are required for their intact isolation from the source material and for their analysis, both qualitatively and quantitatively. Proteins are also increasingly used as “industrial components,” e.g., in biosensors and immobilized enzymes, because of their specificity, selectivity, and sensitivity. This requires novel and refined proce- ing methods by which the protein isolate can be converted into a form in which it can be utilized.

Computational Peptidology

Peng Zhou,Jian Huang (Biologist)

Author : Peng Zhou,Jian Huang (Biologist)
Publisher : Unknown
Page : 338 pages
File Size : 45,8 Mb
Release : 2015
Category : Computational biology
ISBN : 1493922858

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Computational Peptidology by Peng Zhou,Jian Huang (Biologist) Pdf

In this volume expert researchers detail "in silico" methods widely used to study peptides. These include methods and techniques covering the database, molecular docking, dynamics simulation, data mining, de novo design and structure modeling of peptides and protein fragments. Chapters focus on integration and application of technologies to analyze, model, identify, predict, and design a wide variety of bioactive peptides, peptide analogues and peptide drugs, as well as peptide-based biomaterials. Written in the highly successful "Methods in Molecular Biology" series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, "Computational Peptidology" seeks to aid scientists in the further study into this newly rising subfield.

Peptide Analysis Protocols

Ben M. Dunn,Michael W. Pennington

Author : Ben M. Dunn,Michael W. Pennington
Publisher : Taylor & Francis US
Page : 352 pages
File Size : 40,8 Mb
Release : 1994-11-07
Category : Medical
ISBN : 0896032744

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Peptide Analysis Protocols by Ben M. Dunn,Michael W. Pennington Pdf

In this second volume of a two-volume set, state-of-the-art methods are given for the analysis of peptides. Leading authorities provide reproducible protocols for peptide analysis using HPLC, capillary zone electrophoresis, mass spectrometry, UV and fluorescence spectroscopy, to name a few. The authors describe the procedures in a practical, step-by-step fashion suitable for both experts or novices. Chapters also include a helpful Notes section offering troubleshooting tips, alternative procedures, details on substituting unavailable materials, and informative explanations about why certain steps are necessary. These extra measures help to guarantee the successful outcome of your experiments. Dunn and Pennington's Peptide Analysis Protocols offers a comprehensive collection of standard, readily reproducible methods for many procedures that will enable scientists in every area of biochemical and biological research to analyze those peptides that have been synthesized in the laboratory.

Biophysical Characterization Of Functional Peptides

Biophysical Characterization of Functional Peptides by Vibin Ramakrishnan Pdf

The Peptides Analysis, Synthesis, Biology by Erhard Gross Pdf

Biophysical Characterization of Proteins in Developing Biopharmaceuticals by Damian J. Houde,Steven A. Berkowitz Pdf

Major Methods of Peptide Bond Formation by Erhard Gross,Johannes Meienhofer Pdf

Collagen Mimetic Peptides and Their Biophysical Characterization by Jianxi Xiao Pdf

Polypeptide Materials by Maxim G. Ryadnov Pdf

Peptides by Bernd Gutte Pdf

Protein Structure Analysis by Roza Maria Kamp,Theodora Choli-Papadopoulou,Brigitte Wittmann-Liebold Pdf

Physical Biology of Proteins and Peptides by Luis Olivares-Quiroz,Orlando Guzmán-López,Hector Eduardo Jardón-Valadez Pdf

Peptides Frontiers of Peptide Science by James P. Tam,Pravin T.P. Kaumaya Pdf

Solid-Phase Peptide Synthesis by Gregg B. Fields Pdf

Ion Channels: Channel Chemical Biology, Engineering, and Physiological Function by Anonim Pdf

Characterization of Proteins by Felix Franks Pdf

Computational Peptidology by Peng Zhou,Jian Huang (Biologist) Pdf

Peptide Analysis Protocols by Ben M. Dunn,Michael W. Pennington Pdf

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