Chemistry Of Protein And Nucleic Acid Cross Linking And Conjugation Second Edition

Author : Shan S. Wong,David M. Jameson
Publisher : CRC Press
Page : 624 pages
File Size : 50,5 Mb
Release : 2011-10-10
Category : Medical
ISBN : 9780849374913

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Chemistry of Protein and Nucleic Acid Cross-Linking and Conjugation, Second Edition by Shan S. Wong,David M. Jameson Pdf

Since the publication of the first edition of Chemistry of Protein Conjugation and Cross-Linking in 1991, new cross-linking reagents, notably multifunctional cross-linkers, have been developed and synthesized. The completion of the human genome project has opened a new area for studying nucleic acid and protein interactions using nucleic acid cross-linking reagents, and advances have also been made in the area of biosensors and microarray biochips for the detection and analysis of genes, proteins, and carbohydrates. In addition, developments in physical techniques with unprecedented sensitivity and resolution have facilitated the analysis of cross-linked products. Updated to reflect the advances of the 21st century, this book offers: An overview of the chemical principles underlying the processes of cross-linking and conjugation A thorough list of cross-linking reagents published in the literature since the first edition, covering monofunctional, homobifunctional, heterobifunctional, multifunctional, and zero-length cross-linkers Reviews of the use of these reagents in studying protein tertiary structures, geometric arrangements of subunits within complex proteins and nucleic acids, near-neighbor analysis, protein-to-protein or ligand–receptor interactions, and conformational changes of biomolecules Discusses the application of immunoconjugation for immunoassays, immunotoxins for targeted therapy, microarray technology for analysis of various biomolecules, and solid state chemistry for immobilizations

Author : Shan S. Wong
Publisher : CRC Press
Page : 360 pages
File Size : 52,5 Mb
Release : 1991-06-18
Category : Medical
ISBN : 0849358868

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Chemistry of Protein Conjugation and Cross-Linking by Shan S. Wong Pdf

Chemical cross-linking reagents have attained great practical use in industry as well as in basic research, and an understanding of their fundamental principles of reaction is paramount to their applications. With broad coverage of the development and application of these reagents, Chemistry of Protein Conjugation and Cross-Linking discusses the mechanism of reaction and allows you to put the theory into practice. The book offers an explanation of the underlying mechanism of chemical modification, surveys all the bifunctional reagents used in bioconjugation and cross-linking, and provides a review of practical applications of these reagents in various areas of biochemistry, molecular biology, biotechnology, nucleic acid chemistry, immunochemistry, and diagnostic and biomedical disciplines. It contains numerous examples and illustrations, plus step-by-step explanations to reaction procedures. It is an excellent introduction and a comprehensive reference about chemical modification.

Author : Greg T. Hermanson
Publisher : Academic Press
Page : 1166 pages
File Size : 48,8 Mb
Release : 2013-07-25
Category : Science
ISBN : 9780123822406

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Bioconjugate Techniques by Greg T. Hermanson Pdf

Bioconjugate Techniques, Third Edition, is the essential guide to the modification and cross linking of biomolecules for use in research, diagnostics, and therapeutics. It provides highly detailed information on the chemistry, reagent systems, and practical applications for creating labeled or conjugate molecules. It also describes dozens of reactions, with details on hundreds of commercially available reagents and the use of these reagents for modifying or crosslinking peptides and proteins, sugars and polysaccharides, nucleic acids and oligonucleotides, lipids, and synthetic polymers. Offers a one-stop source for proven methods and protocols for synthesizing bioconjugates in the lab Provides step-by-step presentation makes the book an ideal source for researchers who are less familiar with the synthesis of bioconjugates Features full color illustrations Includes a more extensive introduction into the vast field of bioconjugation and one of the most thorough overviews of immobilization chemistry ever presented

Author : J. Miguel Oliveira,Joana Silva-Correia,Rui L. Reis
Publisher : Academic Press
Page : 851 pages
File Size : 54,8 Mb
Release : 2023-09-29
Category : Science
ISBN : 9780128242254

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Hydrogels for Tissue Engineering and Regenerative Medicine by J. Miguel Oliveira,Joana Silva-Correia,Rui L. Reis Pdf

Hydrogels for Tissue Engineering and Regenerative Medicine: From Fundaments to Applications provides the reader with a comprehensive, concise and thoroughly up-to-date resource on the different types of hydrogels in tissue engineering and regenerative medicine. The book is divided into three main sections that describe biological activities and the structural and physicochemical properties of hydrogels, along with a wide range of applications, including their combination with emerging technologies. Written by a diverse range of international academics for professionals, researchers, undergraduate and graduate students, this groundbreaking publication fills a gap in literature needed in the tissue engineering and regenerative medicine field. Reviews the fundamentals and recent advances of hydrogels in tissue engineering and regenerative medicine applications Presents state-of-the-art methodologies for the synthesis and processing of different types of hydrogels Includes contributions by leading experts in engineering, the life sciences, microbiology and clinical medicine

Author : Leon P. Bignold
Publisher : Academic Press
Page : 686 pages
File Size : 55,7 Mb
Release : 2019-11-01
Category : Medical
ISBN : 9780128173299

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Principles of Tumors by Leon P. Bignold Pdf

Principles of Tumors: A Translational Approach to Foundations, Second Edition, provides a concise summary of translational/interdisciplinary topics on the various aspects of tumors, especially abnormalities in their cells, their causes and effects on patients. Topics discussed include how genomic abnormalities in tumors may result from the actions of carcinogens and how genomic changes determine the cell biological/morphological abnormalities in tumor cell populations. In addition, the relationships between tumor cell genomics and therapeutic outcomes are described. There are also supporting appendices on general bioscience, including the principles of histology (the cells and tissues of the body), genetics, pathology, radiology and pharmacology. This book gives a thorough, detailed, yet concise account of the main bioscience, clinical and therapeutic aspects of tumors. It emphasizes the translational aspects of research into tumors with extensive discussions of interdisciplinary issues. The content in this book will be invaluable for researchers and clinicians involved in collaborative projects where it is necessary to understand fundamental issues in other branches of biomedicine. Presents content that has been totally updated with the most recent developments of the field, including new chapters on tumor imaging exams, new surgical techniques, immunotherapy, gene therapy, and several novel therapies using natural and synthetic compounds Presents translational approaches for every topic to improve conceptual insights for new research projects Covers a broad range of subjects, making it easier for the reader to understand related fields Includes diagrams for complex topics to aid in understanding for non-specialists

Author : Etelka Farkas,Maxim Ryadnov
Publisher : Royal Society of Chemistry
Page : 291 pages
File Size : 45,5 Mb
Release : 2012-08
Category : Reference
ISBN : 9781849734066

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Amino Acids, Peptides and Proteins by Etelka Farkas,Maxim Ryadnov Pdf

In an ever-increasing domain of activity, this annual compilation of the world's research effort provides insight into an important area of biological chemistry.

Author : Sabine M¿ller
Publisher : John Wiley & Sons
Page : 350 pages
File Size : 54,6 Mb
Release : 2008-09-08
Category : Science
ISBN : 9783527622535

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Nucleic Acids from A to Z by Sabine M¿ller Pdf

Concise but complete, this mini-encyclopedia contains over 1,500 entries covering all important concepts, compounds, techniques and acronyms for quick and easy reference. Guiding readers through the ever-increasing jungle of nucleic acid science and technology, the book distills the key information out of the large body of primary literature and presents it in a single volume. A first-stop resource for everyone, from students to established researchers, as both a desktop and library reference.

Author : W. Russ Algar,Philip Dawson,Igor L. Medintz
Publisher : John Wiley & Sons
Page : 430 pages
File Size : 52,7 Mb
Release : 2017-03-17
Category : Technology & Engineering
ISBN : 9783527683475

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Chemoselective and Bioorthogonal Ligation Reactions by W. Russ Algar,Philip Dawson,Igor L. Medintz Pdf

This timely, one-stop reference is the first on an emerging and interdisciplinary topic. Covering both established and recently developed ligation chemistries, the book is divided into two didactic parts: a section that focuses on the details of bioorthogonal and chemoselective ligation reactions at the level of fundamental organic chemistry, and a section that focuses on applications, particularly in the areas of chemical biology, biomaterials, and bioanalysis, highlighting the capabilities and benefits of the ligation reactions. With chapters authored by outstanding scientists who range from trailblazers in the field to young and emerging leaders, this book on a highly interdisciplinary topic will be of great interest for biochemists, biologists, materials scientists, pharmaceutical chemists, organic chemists, and many others.

Author : Roger L. Lundblad
Publisher : CRC Press
Page : 260 pages
File Size : 52,7 Mb
Release : 2005-10-14
Category : Medical
ISBN : 9781000654042

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The Evolution from Protein Chemistry to Proteomics by Roger L. Lundblad Pdf

Largely driven by major improvements in the analytical capability of mass spectrometry, proteomics is being applied to broader areas of experimental biology, ranging from oncology research to plant biology to environmental health. However, while it has already eclipsed solution protein chemistry as a discipline, it is still essentially an extension

Author : Jorge Almodovar
Publisher : Springer
Page : 282 pages
File Size : 43,5 Mb
Release : 2018-01-04
Category : Science
ISBN : 9783319700496

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Electrospun Biomaterials and Related Technologies by Jorge Almodovar Pdf

This books provides a compendium of electrospinning strategies and related technologies for the production of biomaterials for tissue engineering and regenerative medicine applications. It gives a broad overview of the field as well as cutting-edge research on electrospinning and how it is applied to engineer biomaterials. This is an ideal book for biomaterials scientists, engineers, students, and researchers. This book also: Presents cutting-edge research performed in the area of electrospinning with applications in tissue engineering and regenerative medicine Provides readers from the biomaterials field as well as those new to the field with a broad overview of the multiple applications of electrospun biomaterials Summarizes the latest research from the past ten years on electrospinning and related technologies

Author : Phoebe A Rice,Carl C Correll
Publisher : Royal Society of Chemistry
Page : 416 pages
File Size : 46,7 Mb
Release : 2008-04-22
Category : Science
ISBN : 9781847558268

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Protein-Nucleic Acid Interactions by Phoebe A Rice,Carl C Correll Pdf

The structural biology of protein-nucleic acid interactions is in some ways a mature field and in others in its infancy. High-resolution structures of protein-DNA complexes have been studied since the mid 1980s and a vast array of such structures has now been determined, but surprising and novel structures still appear quite frequently. High-resolution structures of protein-RNA complexes were relatively rare until the last decade. Propelled by advances in technology as well as the realization of RNA's importance to biology, the number of example structures has ballooned in recent years. New insights are now being gained from comparative studies only recently made possible due to the size of the database, as well as from careful biochemical and biophysical studies. As a result of the explosion of research in this area, it is no longer possible to write a comprehensive review. Instead, current review articles tend to focus on particular subtopics of interest. This makes it difficult for newcomers to the field to attain a solid understanding of the basics. One goal of this book is therefore to provide in-depth discussions of the fundamental principles of protein-nucleic acid interactions as well as to illustrate those fundamentals with up-to-date and fascinating examples for those who already possess some familiarity with the field. The book also aims to bridge the gap between the DNA- and the RNA- views of nucleic acid - protein recognition, which are often treated as separate fields. However, this is a false dichotomy because protein - DNA and protein - RNA interactions share many general principles. This book therefore includes relevant examples from both sides, and frames discussions of the fundamentals in terms that are relevant to both. The monograph approaches the study of protein-nucleic acid interactions in two distinctive ways. First, DNA-protein and RNA-protein interactions are presented together. Second, the first half of the book develops the principles of protein-nucleic acid recognition, whereas the second half applies these to more specialized topics. Both halves are illustrated with important real life examples. The first half of the book develops fundamental principles necessary to understand function. An introductory chapter by the editors reviews the basics of nucleic acid structure. Jen-Jacobsen and Jacobsen discuss how solvent interactions play an important role in recognition, illustrated with extensive thermodynamic data on restriction enzymes. Marmorstein and Hong introduce the zoology of the DNA binding domains found in transcription factors, and describe the combinational recognition strategies used by many multiprotein eukaryotic complexes. Two chapters discuss indirect readout of DNA sequence in detail: Berman and Lawson explain the basic principles and illustrate them with in-depth studies of CAP, while in their chapter on DNA bending and compaction Johnson, Stella and Heiss highlight the intrinsic connections between DNA bending and indirect readout. Horvath lays out the fundamentals of protein recognition of single stranded DNA and single stranded RNA, and describes how they apply in a detailed analysis of telomere end binding proteins. Nucleic acids adopt more complex structures - Lilley describes the conformational properties of helical junctions, and how proteins recognize and cleave them. Because RNA readily folds due to the stabilizing role of its 2'-hydroxyl groups, Li discusses how proteins recognize different RNA folds, which include duplex RNA. With the fundamentals laid out, discussion turns to more specialized examples taken from important aspects of nucleic acid metabolism. Schroeder discusses how proteins chaperone RNA by rearranging its structure into a functional form. Berger and Dong discuss how topoisomerases alter the topology of DNA and relieve the superhelical tension introduced by other processes such as replication and transcription. Dyda and Hickman show how DNA transposes mediate genetic mobility and Van Duyne discusses how site-specific recombinases "cut" and "paste" DNA. Horton presents a comprehensive review of the structural families and chemical mechanisms of DNA nucleases, whereas Li in her discussion of RNA-protein recognition also covers RNA nucleases. Lastly, FerrÚ-D'AmarÚ shows how proteins recognize and modify RNA transcripts at specific sites. The book also emphasises the impact of structural biology on understanding how proteins interact with nucleic acids and it is intended for advanced students and established scientists wishing to broaden their horizons.

Author : Mohammed Aslam,Alastair Dent
Publisher : Unknown
Page : 856 pages
File Size : 41,8 Mb
Release : 1998
Category : Biochemistry
ISBN : PSU:000056868074

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Bioconjugation by Mohammed Aslam,Alastair Dent Pdf

Recent years have seen an enormous increase in the use of protein reagents in the biological and medical sciences. Enzyme conjugates and immobilised proteins are fundamental to many diagnostic applications, and existing therapeutic advances are being made using a range of approaches based on antibody conjugates. Protein conjugation techniques are central to the development of these reagents.

Author : Greg T. Hermanson
Publisher : Academic Press
Page : 785 pages
File Size : 45,5 Mb
Release : 1996-01-15
Category : Science
ISBN : 9780080572123

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Bioconjugate Techniques by Greg T. Hermanson Pdf

Bioconjugate Techniques is the essential guide to the modification and crosslinking of biomolecules for use in research, diagnostics, and therapeutics. It provides highly detailed information on the chemistry, reagent systems, and practical applications for creating labeled or conjugate molecules. It also describes dozens of reactions with details on hundreds of commercially available reagents and the use of these reagents for modifying or crosslinking peptides and proteins, sugars and polysaccharides, nucleic acids and oligonucleotides, lipids, and synthetic polymers. Armed with this information and the abundant protocols provided, readers will form unique complexes that can be used for detecting, quantifying, and targeting important analytes. This book helps readers make: high activity antibody-enzymes conjugates, immunotoxins, immunogen complexes, liposome conjugates; as well as biotinylated molecules, avidin or streptavidin conjugates, colloidal gold labeled proteins, PEG or dextran complexes, labeled oligonucleotide probes, and fluorescently tagged or radiolabeled molecules. This book is the first to thoroughly capture the entire field of bioconjugate chemistry in a single volume Serves as a practical guide to modification and cross-linking technology for research, diagnostics, and therapeutics Provides useful, detailed, easy-to-follow, step-by-step protocols Contains easy-to-read, and easy-to-understand key concepts for making bioconjugates of all types Efficiently covers the chemistry of bioconjugation, the major reagents available for modification and cross-linking, and the application of these reagents to the synthesis of highly active conjugates Cites over more than references keyed to concepts covered in the book Uses more than 600 figures to illustrate bioconjugate reagents, their reactions, and applications Suggests sources for all key reagents

Author : Anonim
Publisher : Unknown
Page : 128 pages
File Size : 43,8 Mb
Release : 1999
Category : Electronic
ISBN : OCLC:64147745

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Protein-nucleic acid interactions by Anonim Pdf

Author : Anonim
Publisher : Elsevier
Page : 368 pages
File Size : 47,9 Mb
Release : 2013-08-14
Category : Science
ISBN : 9780124116276

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Dynamics of Proteins and Nucleic Acids by Anonim Pdf

Published continuously since 1944, Advances in Protein Chemistry and Structural Biology has been a continuous, essential resource for protein chemists. Covering reviews of methodology and research in all aspects of protein chemistry, including purification/expression, proteomics, modeling and structural determination and design, each volume brings forth new information about protocols and analysis of proteins while presenting the most recent findings from leading experts in a broad range of protein-related topics. Covers reviews of methodology and research in all aspects of protein chemistry Brings forth new information about protocols and analysis of proteins while presenting the most recent findings from leading experts in a broad range of protein-related topics