Drosophila Protocols

Author : William Sullivan,M. Ashburner,R. Scott Hawley
Publisher : CSHL Press
Page : 798 pages
File Size : 45,7 Mb
Release : 2000
Category : Medical
ISBN : 0879695862

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Drosophila Protocols by William Sullivan,M. Ashburner,R. Scott Hawley Pdf

This exceptional laboratory manual describes thirty-seven procedures most likely to be used in the next decade for molecular, biochemical, and cellular studies on Drosophila. They were selected after extensive consultation with the research community and rigorously edited for clarity, uniformity, and conciseness.The methods included permit investigation of chromosomes, cell biology, molecular biology, genomes, biochemistry, and development. Each protocol includes the basic information needed by novices, with sufficient detail to be valuable to experienced investigators. Each method is carefully introduced and illustrated with figures, tables, illustrations, and examples of the data obtainable. The book's appendices include key aspects of Drosophila biology, essential solutions, buffers, and recipes.An evolution of Michael Ashburner's 1989 classic Drosophila: A Laboratory Manual, this book is an essential addition to the personal library of Drosophila investigators and an incomparable resource for other research groups with goals likely to require fly-based technical approaches.

Author : Daryl S. Henderson
Publisher : Springer Science & Business Media
Page : 467 pages
File Size : 40,5 Mb
Release : 2008-02-03
Category : Science
ISBN : 9781592596652

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Drosophila Cytogenetics Protocols by Daryl S. Henderson Pdf

Leading drosophilists describe in step-by-step detail all the essential techniques for studying Drosophila chromosomes and suggest new avenues for scientific exploration. The chapters emphasize specimen preparation (from dissection to mounting) and cover both polytene and mitotic/meiotic chromosomes in depth. Each fully tested and readily reproducible protocol offers a background introduction, equipment and reagent lists, and tips on troubleshooting and avoiding pitfalls. A cutting-edge FISH and immunolocalization technique will be important for discovering how DNA sequence influences higher-order chromosome architecture and ultimately gene expression.

Author : Christian Dahmann
Publisher : Springer Nature
Page : 423 pages
File Size : 45,9 Mb
Release : 2022-08-18
Category : Science
ISBN : 9781071625415

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Drosophila by Christian Dahmann Pdf

This third edition expands on the previous editions by providing updated protocols and current methods that cover recent breakthroughs in Drosophila research. Chapters guide readers through FlyBase, versatile gene expression systems, analysis of microRNA function, single-cell transcriptome data and metabolism, recent applications of CRISPR for precise genome editing, transcriptional activation and cell lineage tracing, protein inhibition and tagging, optogenetic and optochemical control of tissue mechanics, AFM measurements, sample preparation for electron microscopy, live imaging of different tissues and organs, and quantitative image analysis. Written in the format of the highly successful Methods in Molecular Biology series, chapters include an introduction to their topic, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls Authoritative and cutting-edge, Drosophila: Methods and Protocols, Third Edition serves as a useful and practical guide to new researchers and experts using Drosophila as a model system. Chapter 7 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Author : William Sullivan,Michael Ashburner,R. Scott Hawley
Publisher : Unknown
Page : 697 pages
File Size : 44,7 Mb
Release : 2000
Category : Drosophila
ISBN : 7030124405

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Drosophila Protocols by William Sullivan,Michael Ashburner,R. Scott Hawley Pdf

Author : Michelle S. Giedt,Tina L. Tootle
Publisher : Springer Nature
Page : 447 pages
File Size : 46,6 Mb
Release : 2023-01-30
Category : Science
ISBN : 9781071629703

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Drosophila Oogenesis by Michelle S. Giedt,Tina L. Tootle Pdf

This detailed volume compiles numerous methods to explore fly oogenesis. Beginning with updated protocols from isolating and staining the ovary to numerous imaging techniques and genetic protocols for cell-specific assessment and CRISPR-mediated mutagenesis, the volume continues by detailing techniques from assessing cytoskeletal structures to uncovering protein-protein interactions, with closing chapters exploring how Drosophila oogenesis can be used in the classroom and in outreach programs to increase interest in biomedical research, STEM education, and STEM careers. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Drosophila Oogenesis: Methods and Protocols serves as an ideal guide for both new and expert researchers working with this robust and versatile model organism.

Author : Christian Dahmann
Publisher : Humana
Page : 0 pages
File Size : 42,8 Mb
Release : 2016-10-12
Category : Medical
ISBN : 1493963694

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Drosophila by Christian Dahmann Pdf

This second edition volume expands on the previous edition by presenting updated protocols for several of the techniques described in the first edition of Drosophila: Methods and Protocols and current methods that cover recent breakthroughs in Drosophila research. The book begins with a description of FlyBase--a database of genes and genomes--followed by the presentation of systems for versatile gene expression in the fly. The first few chapters in this book detail gene knockdown and editing, including CRISPR-Cas9 and protein knockdown. The next few chapters are devoted to methods describing live imaging of different tissues and organs, followed by chapters on how to quantify image data and how to probe tissue mechanics by laser ablation. The next two chapters provide methods for analyzing transcription followed by protocols to study growth, metabolism, ageing, and behavior in Drosophila. This volume concludes with chapters on electrophysiological recordings and methods to establish cell lines. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Drosophila: Methods and Protocols, Second Edition is a valuable source of hands-on protocols and reviews for molecular, cell, and developmental biologists using Drosophila as model systems in their work.

Author : Charles R. Dearolf
Publisher : Unknown
Page : 350 pages
File Size : 43,7 Mb
Release : 2005-07-01
Category : Science
ISBN : 1588292398

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Drosophila Protocols by Charles R. Dearolf Pdf

Author : Bing Zhang,Marc R. Freeman,Scott Waddell
Publisher : Unknown
Page : 552 pages
File Size : 41,9 Mb
Release : 2010
Category : Drosophila
ISBN : STANFORD:36105215501920

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Drosophila Neurobiology by Bing Zhang,Marc R. Freeman,Scott Waddell Pdf

Based on Cold Spring Harbor Laboratory's long-running course, Drosophila Neurobiology: A Laboratory Manual offers detailed protocols and background material for researchers interested in using Drosophila as an experimental model for investigating the nervous system. This manual covers three approaches to the field: analysis of neural development, recording and imaging activities in the nervous system, and analysis of behavior. Techniques described include molecular, genetic, electrophysiological, imaging, behavioral and developmental methods.

Author : William Sullivan,M. Ashburner,R. Scott Hawley
Publisher : Unknown
Page : 736 pages
File Size : 54,6 Mb
Release : 2000
Category : Drosophila
ISBN : UOM:39015049979241

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Drosophila Protocols by William Sullivan,M. Ashburner,R. Scott Hawley Pdf

Author : Masamitsu Yamaguchi
Publisher : Springer
Page : 308 pages
File Size : 54,9 Mb
Release : 2018-06-27
Category : Medical
ISBN : 9789811305290

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Drosophila Models for Human Diseases by Masamitsu Yamaguchi Pdf

Most biological pathways, physical and neurological properties are highly conserved between humans and Drosophila and nearly 75% of human disease-causing genes have a functional homologue in Drosophila. This volume provides recent advances in Drosophila models for various human diseases, with each chapter providing a review of studies involving Drosophila models, as well as detailed protocols commonly used in laboratories. Starting with a review of Drosophila’s value as a highly tractable model organism for studying human diseases, subsequent chapters present Drosophila models for specific human diseases. The book provides a useful resource for all scientists who are starting to use the Drosophila model in their studies, and for researchers working in the pharmaceutical industry and using new screening models to develop new medicines for various diseases.

Author : Hugh J. M. Brady
Publisher : Springer Science & Business Media
Page : 306 pages
File Size : 44,8 Mb
Release : 2008-02-05
Category : Science
ISBN : 9781592598120

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Apoptosis Methods and Protocols by Hugh J. M. Brady Pdf

The most fundamental question facing each and every cell within an org- ism is to survive or to die. Cell death is required for normal function; some estimates suggest that as many as one million cells undergo cell death every second in the adult human body. Almost all cells undergoing physiological, or programmed, cell death, independent of cell type, manifest a stereotypic p- tern of morphological changes termed apoptosis. Typically, apoptotic cells d- play shrinkage, membrane blebbing, chromatin condensation, and nuclear fragmentation. The integrity of the cell membrane is not lost during apoptosis and so avoids eliciting the inflammatory response that would have been caused by the spillage of the cell’s contents. This is quite in contrast to the loss of cell contents typical of necrosis. The caspases, the family of intracellular cysteine proteases associated with apoptosis, are responsible for the stereotypical m- phological changes. Caspases cleave various substrate proteins that act on DNA fragmentation, nuclear envelope integrity, the cytoskeleton, and cell volume regulation. Apoptotic cells are cleared in vivo by the process of phagocytosis, in which specific “phagocytes” move to the site of apoptosis, engulf the dying cells and digest them. Apoptosis has a central role in many physiological processes, for example, in the immune system. Autoreactive cells are deleted via apoptosis to prevent autoimmunity. At the end of an immune response, activated lymphocytes are removed to maintain homeostasis within the immune system.

Author : Monalisa Mishra
Publisher : Springer
Page : 265 pages
File Size : 42,7 Mb
Release : 2021-02-01
Category : Science
ISBN : 1493997580

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Fundamental Approaches to Screen Abnormalities in Drosophila by Monalisa Mishra Pdf

This volume includes in-depth, hands-on protocols for detecting various developmental defects in Drosophila. It provides cutting-edge methods for maintaining Drosophila under laboratory conditions to perform various experiments, and for dissecting various imaginal discs of the larvae. Further, biochemical protocols for estimating the levels of different metabolites, reactive oxygen species, and fat-sensitive pathways are discussed, and various staining and behavior techniques for fat detection are provided. The book explains how various fluorescent dyes and the comet assay can be used to identify DNA damage, and elaborates on the analysis of the eye, antennae, imaginal disc, gut and muscle damage under bright field and fluorescent microscopes. It covers the analysis of hemolymph using Giemsa staining; determining the functionality of channel proteins, eye and mechanosensory organs in adults and larvae using various behavioral assays; and metal detection and structural analysis for various tissues using SEM. In closing, it addresses the analysis of Drosophila’s eye and head via paraffin section; measurement of reactive oxygen species from various tissues via FACS; and the CRISPER technique for gene editing and analysis of micro RNA mutations.

Author : Anonim
Publisher : Academic Press
Page : 789 pages
File Size : 50,7 Mb
Release : 1995-01-25
Category : Science
ISBN : 9780080859422

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Drosophila melanogaster: Practical Uses in Cell and Molecular Biology by Anonim Pdf

Drosophila melanogaster: Practical Uses in Cell and Molecular Biology is a compendium of mostly short technical chapters designed to provide state-of-the art methods to the broad community of cell biologists, and to put molecular and cell biological studies of flies into perspective. The book makes the baroque aspects of genetic nomenclature and procedure accessible to cell biologists. It also contains a wealth of technical information for beginning or advanced Drosophila workers. Chapters, written within a year of publication, make this topical volume a valuable laboratory guide today and an excellent general reference for the future. Key Features* Collection of ready-to-use, state-of-the art methods for modern cell biological and related research using Drosophila melanogaster* Accessible to both experienced Drosophila researchers and to others who wish to join in at the cutting edge of this system * Drosophila offers an easily managed life cycle, inexpensive lifestyle, extraordinarily manipulable molecular and classical genetics, now combined with powerful new cell biology techniques * Introduction and overview sections orient the user to the Drosophila literature and lore * Six full-color plates and over 100 figures and tables enhance the understanding of these cell biology techniques

Author : Dr. Tim Doran,Chris Helliwell
Publisher : CABI
Page : 269 pages
File Size : 47,9 Mb
Release : 2009
Category : Science
ISBN : 9781845934101

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RNA Interference by Dr. Tim Doran,Chris Helliwell Pdf

This methods manual provides an introduction to RNA interference, the theory behind its many applications, and specific protocols for RNAi, in organisms from plants and C.elegans to Drosophila and mammals. There are also chapters covering small hairpin RNAs and viral-induced gene silencing.

Author : Ian A. Darby
Publisher : Taylor & Francis US
Page : 370 pages
File Size : 54,7 Mb
Release : 2000
Category : Medical
ISBN : 0896036863

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In Situ Hybridization Protocols by Ian A. Darby Pdf

Annotation Darby (human biology, RMIT U., Victoria, Australia) is joined by geneticists, molecular biologists, and pathologists from around the world to describe basic and advanced techniques for hybridization, for whole-mount embryo specimens and at the electron microscope level. Coverage includes protocols for detection of DNA fragmentation in apoptosis, localization of genes to particular chromosomes, and the use of DNA and RNA probes to detect expression in cells or tissue sections. For novice and experienced investigators who need proven and readily reproducible methods. Annotation c. Book News, Inc., Portland, OR (booknews.com)