Fundamental Approaches To Screen Abnormalities In Drosophila

Author : Monalisa Mishra
Publisher : Springer
Page : 0 pages
File Size : 43,6 Mb
Release : 2020-01-18
Category : Science
ISBN : 1493997556

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Fundamental Approaches to Screen Abnormalities in Drosophila by Monalisa Mishra Pdf

This volume includes in-depth, hands-on protocols for detecting various developmental defects in Drosophila. It provides cutting-edge methods for maintaining Drosophila under laboratory conditions to perform various experiments, and for dissecting various imaginal discs of the larvae. Further, biochemical protocols for estimating the levels of different metabolites, reactive oxygen species, and fat-sensitive pathways are discussed, and various staining and behavior techniques for fat detection are provided. The book explains how various fluorescent dyes and the comet assay can be used to identify DNA damage, and elaborates on the analysis of the eye, antennae, imaginal disc, gut and muscle damage under bright field and fluorescent microscopes. It covers the analysis of hemolymph using Giemsa staining; determining the functionality of channel proteins, eye and mechanosensory organs in adults and larvae using various behavioral assays; and metal detection and structural analysis for various tissues using SEM. In closing, it addresses the analysis of Drosophila’s eye and head via paraffin section; measurement of reactive oxygen species from various tissues via FACS; and the CRISPER technique for gene editing and analysis of micro RNA mutations.

Author : Bruce Alberts
Publisher : Unknown
Page : 0 pages
File Size : 51,9 Mb
Release : 2002
Category : Cytology
ISBN : 0815332181

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Molecular Biology of The Cell by Bruce Alberts Pdf

Author : National Research Council,Commission on Life Sciences,Board on Environmental Studies and Toxicology,Committee on Developmental Toxicology
Publisher : National Academies Press
Page : 347 pages
File Size : 51,5 Mb
Release : 2000-11-21
Category : Nature
ISBN : 9780309171809

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Scientific Frontiers in Developmental Toxicology and Risk Assessment by National Research Council,Commission on Life Sciences,Board on Environmental Studies and Toxicology,Committee on Developmental Toxicology Pdf

Scientific Frontiers in Developmental Toxicology and Risk Assessment reviews advances made during the last 10-15 years in fields such as developmental biology, molecular biology, and genetics. It describes a novel approach for how these advances might be used in combination with existing methodologies to further the understanding of mechanisms of developmental toxicity, to improve the assessment of chemicals for their ability to cause developmental toxicity, and to improve risk assessment for developmental defects. For example, based on the recent advances, even the smallest, simplest laboratory animals such as the fruit fly, roundworm, and zebrafish might be able to serve as developmental toxicological models for human biological systems. Use of such organisms might allow for rapid and inexpensive testing of large numbers of chemicals for their potential to cause developmental toxicity; presently, there are little or no developmental toxicity data available for the majority of natural and manufactured chemicals in use. This new approach to developmental toxicology and risk assessment will require simultaneous research on several fronts by experts from multiple scientific disciplines, including developmental toxicologists, developmental biologists, geneticists, epidemiologists, and biostatisticians.

Author : Farzana Khan Perveen
Publisher : BoD – Books on Demand
Page : 270 pages
File Size : 41,5 Mb
Release : 2018-02-28
Category : Technology & Engineering
ISBN : 9789535138532

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Drosophila melanogaster by Farzana Khan Perveen Pdf

This book contains 12 chapters divided into two sections. Section 1 is "Drosophila - Model for Genetics." It covers introduction, chromosomal polymorphism, polytene chromosomes, chromosomal inversion, chromosomal evolution, cell cycle regulators in meiosis and nongenetic transgenerational inheritance in Drosophila. It also includes ecological genetics, wild-type strains, morphometric analysis, cytostatics, frequencies of early and late embryonic lethals (EEL and LEL) and mosaic imaginal discs of Drosophila for genetic analysis in biomedical research. Section 2 is "Drosophila - Model for Therapeutics." It explains Drosophila as model for human diseases, neurodegeneration, heart-kidney metabolic disorders, cancer, pathophysiology of Parkinson's disease, dopamine, neuroprotective therapeutics, mitochondrial dysfunction and translational research. It also covers Drosophila role in ubiquitin-carboxyl-terminal hydrolase-L1 (UCH-L1) protein, eye development, anti-dUCH antibody, neuropathy target esterase (NTE), organophosphorous compound-induced delayed neuropathy (OPIDN) and hereditary spastic paraplegia (HSP). It also includes substrate specificities, kinetic parameters of recombinant glutathione S-transferases E6 and E7 (DmGSTE6 and DmGSTE7), detoxification and insecticidal resistance and antiviral immunity in Drosophila.

Author : Helen Sink
Publisher : Springer Science & Business Media
Page : 214 pages
File Size : 52,5 Mb
Release : 2007-02-26
Category : Science
ISBN : 9780387329635

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Muscle Development in Drosophilia by Helen Sink Pdf

The different aspects of muscle development are considered from cellular, molecular and genetic viewpoints, and the text is supported by black/white and color illustrations. The book will appeal to those studying muscle development and muscle biology in any organism.

Author : National Research Council,Institute for Laboratory Animal Research,International Committee of the Institute for Laboratory Animal Research
Publisher : National Academies Press
Page : 166 pages
File Size : 45,9 Mb
Release : 2000-10-30
Category : Science
ISBN : 9780309171472

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Microbial Status and Genetic Evaluation of Mice and Rats by National Research Council,Institute for Laboratory Animal Research,International Committee of the Institute for Laboratory Animal Research Pdf

US/Japan meetings on laboratory animal science have been held virtually every year since 1980 under the US/Japan Cooperative Program on Science and Technology. Over the years these meetings have resulted in a number of important documents including the Manual of Microbiologic Monitoring of Laboratory Animals published in 1994 and the article Establishment and Preservation of Reference Inbred Strains of Rats for General Purposes. In addition to these publications, the meetings have been instrumental in increasing awareness of the need for microbiologic monitoring of laboratory rodents and the need for genetic definition and monitoring of mice and rats. In cooperation with the Comparative Medicine section of NCRR/NIH, the ILAR Council and staff are pleased to become the host for this important annual meeting and look forward to participating in future meetings. The support and sponsorship of NCRR (P40 RR 11611) in the United States and the Central Institute for Experimental Animals in Japan are gratefully acknowledged. Bringing together the leading scientists in the field of laboratory animal care has resulted in increased understanding of American and Japanese approaches to laboratory animal science and should continue to strengthen efforts to harmonize approaches aimed at resolving common challenges in the use of animal models for biomedical research and testing. This effort to improve understanding and cooperation between Japan and the United States should also be useful in developing similar interaction with other regions of the world including Europe, Australia, and Southeast Asia.

Author : Charles J. Epstein,Robert P. Erickson,Anthony Joseph Wynshaw-Boris
Publisher : Oxford University Press, USA
Page : 1110 pages
File Size : 43,9 Mb
Release : 2004
Category : Medical
ISBN : 9780195145021

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Inborn Errors of Development by Charles J. Epstein,Robert P. Erickson,Anthony Joseph Wynshaw-Boris Pdf

In this book, the clinical chapters are organized into sections by defined developmental pathways or gene families, and each section is preceded by a general overview. For each disorder the authors cover the disease-causing genes, the role of these genes in development as elucidated in model organisms, the human mutations that have been identified, and the developmental pathogenesis of the condition. Clinical descriptions, along with discussions of therapy and counseling, are provided. This book will be an invaluable resource for physicians, dentists, and other health professionals and for basic scientists interested in developmental processes and genetic perturbations that affect them.

Author : Amit Singh,Madhuri Kango-Singh
Publisher : Springer Nature
Page : 368 pages
File Size : 47,5 Mb
Release : 2020-05-18
Category : Medical
ISBN : 9783030422462

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Molecular Genetics of Axial Patterning, Growth and Disease in Drosophila Eye by Amit Singh,Madhuri Kango-Singh Pdf

Drosophila melanogaster (fruit fly) is a highly versatile model with a genetic legacy of more than a century. It provides powerful genetic, cellular, biochemical and molecular biology tools to address many questions extending from basic biology to human diseases. One of the most important questions in biology is how a multi-cellular organism develops from a single-celled embryo. The discovery of the genes responsible for pattern formation has helped refine this question and has led to other questions, such as the role of various genetic and cell biological pathways in regulating the process of pattern formation and growth during organogenesis. The Drosophila eye model has been extensively used to study molecular genetic mechanisms involved in patterning and growth. Since the genetic machinery involved in the Drosophila eye is similar to humans, it has been used to model human diseases and homology to eyes in other taxa. This updated second edition covers current progress in the study of molecular genetic mechanisms of pattern formation, mutations in axial patterning, genetic regulation of growth, and more using the Drosophila eye as a model.

Author : Alfred Henry Sturtevant
Publisher : CSHL Press
Page : 190 pages
File Size : 41,8 Mb
Release : 2001
Category : Medical
ISBN : 0879696079

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A History of Genetics by Alfred Henry Sturtevant Pdf

In the small “Fly Room†at Columbia University, T.H. Morgan and his students, A.H. Sturtevant, C.B. Bridges, and H.J. Muller, carried out the work that laid the foundations of modern, chromosomal genetics. The excitement of those times, when the whole field of genetics was being created, is captured in this book, written in 1965 by one of those present at the beginning. His account is one of the few authoritative, analytic works on the early history of genetics. This attractive reprint is accompanied by a website, http://www.esp.org/books/sturt/history/ offering full-text versions of the key papers discussed in the book, including the world's first genetic map.

Author : Anonim
Publisher : Academic Press
Page : 0 pages
File Size : 44,7 Mb
Release : 2013-09-04
Category : Science
ISBN : 0123969689

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Developmental Timing by Anonim Pdf

This new volume of Current Topics in Developmental Biology covers developmental timing, with contributions from an international board of authors. The chapters provide a comprehensive set of reviews covering such topics as the timing of developmental programs in Drosophila, temporal patterning of neural progenitors, and environmental modulation of developmental timing.

Author : Therese A. Markow,Patrick O'Grady
Publisher : Elsevier
Page : 272 pages
File Size : 54,6 Mb
Release : 2005-11-01
Category : Science
ISBN : 0080454097

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Drosophila by Therese A. Markow,Patrick O'Grady Pdf

Anyone wishing to tap the research potential of the hundreds of Drosophila species in addition to D.melanogaster will finally have a single comprehensive resource for identifying, rearing and using this diverse group of insects. This is the only group of higher eukaryotes for which the genomes of 12 species have been sequenced. The fruitfly Drosophila melanogaster continues to be one of the greatest sources of information regarding the principles of heredity that apply to all animals, including humans. In reality, however, over a thousand different species of Drosophila exist, each with the potential to make their own unique contributions to the rapidly changing fields of genetics and evolution. This book, by providing basic information on how to identify and breed these other fruitflies, will allow investigators to take advantage, on a large scale, of the valuable qualities of these other Drosophila species and their newly developed genomic resources to address critical scientific questions. * Provides easy to use keys and illustrations to identify different Drosophila species * A guide to the life history differences of hundreds of species * Worldwide distribution maps of hundreds of species * Complete recipes for different Drosophila diets * Offers an analysis on how to account for species differences in designing and conducting experiments * Presents useful ideas of how to collect the many different Drosophila species in the wild

Author : Mousumi Mutsuddi,Ashim Mukherjee
Publisher : Springer Nature
Page : 470 pages
File Size : 49,7 Mb
Release : 2019-12-05
Category : Medical
ISBN : 9789811322181

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Insights into Human Neurodegeneration: Lessons Learnt from Drosophila by Mousumi Mutsuddi,Ashim Mukherjee Pdf

This book is aimed at generating an updated reservoir of scientific endeavors undertaken to unravel the complicated yet intriguing topic of neurodegeneration. Scientists from Europe, USA and India who are experts in the field of neurodegenerative diseases have contributed to this book. This book will help readers gain insight into the recent knowledge obtained from Drosophila model, in understanding the molecular mechanisms underlying neurodegenerative disorders and also unravel novel scopes for therapeutic interventions. Different methodologies available to create humanized fly models that faithfully reflects the pathogenicities associated with particular disorders have been described here. It also includes information on the exciting area of neural stem cells. A brief discussion on neurofibrillary tangles, precedes the elaborate description of lessons learnt from Drosophila about Alzheimer's, Parkinson’s, Spinomuscular Atrophy, Huntington’s diseases, RNA expansion disorders and Hereditary Spastic Paraplegia. We have concluded the book with the use of Drosophila for identifying pharmacological therapies for neurodegenerative disorders. The wide range of topics covered here will not only be relevant for beginners who are new to the concept of the extensive utility of Drosophila as a model to study human disorders; but will also be an important contribution to the scientific community, with an insight into the paradigm shift in our understanding of neurodegenerative disorders. Completed with informative tables and communicative illustrations this book will keep the readers glued and intrigued. We have comprehensively anthologized the lessons learnt on neurodegeneration from Drosophila and have thus provided an insight into the multidimensional aspects of pathogenicities of majority of the neurodegenerative disorders.

Author : Christian Dahmann
Publisher : Humana
Page : 0 pages
File Size : 40,8 Mb
Release : 2016-10-12
Category : Medical
ISBN : 1493963694

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Drosophila by Christian Dahmann Pdf

This second edition volume expands on the previous edition by presenting updated protocols for several of the techniques described in the first edition of Drosophila: Methods and Protocols and current methods that cover recent breakthroughs in Drosophila research. The book begins with a description of FlyBase--a database of genes and genomes--followed by the presentation of systems for versatile gene expression in the fly. The first few chapters in this book detail gene knockdown and editing, including CRISPR-Cas9 and protein knockdown. The next few chapters are devoted to methods describing live imaging of different tissues and organs, followed by chapters on how to quantify image data and how to probe tissue mechanics by laser ablation. The next two chapters provide methods for analyzing transcription followed by protocols to study growth, metabolism, ageing, and behavior in Drosophila. This volume concludes with chapters on electrophysiological recordings and methods to establish cell lines. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Drosophila: Methods and Protocols, Second Edition is a valuable source of hands-on protocols and reviews for molecular, cell, and developmental biologists using Drosophila as model systems in their work.

Author : Ralph J. Greenspan
Publisher : CSHL Press
Page : 212 pages
File Size : 45,5 Mb
Release : 2004
Category : Science
ISBN : 0879697113

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Fly Pushing by Ralph J. Greenspan Pdf

A second edition of the classic handbook has become a standard in the Drosophila field. This edition is expanded to include topics in which classical genetic strategies have been augmented with new molecular tools. Included are such new techniques as homologous recombination, RNAi, new mapping techniques, and new mosaic marking techniques.

Author : Josh Dubnau
Publisher : Cambridge University Press
Page : 309 pages
File Size : 52,6 Mb
Release : 2014-06-26
Category : Medical
ISBN : 9781107009035

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Behavioral Genetics of the Fly (Drosophila Melanogaster) by Josh Dubnau Pdf

A comprehensive portrayal of the behaviour genetics of the fruit fly (Drosophila melanogaster) and the methods used in these studies.