Electrophysiological Methods

Author : Franklin Bretschneider,Jan R. de Weille
Publisher : Academic Press
Page : 370 pages
File Size : 41,5 Mb
Release : 2018-11-13
Category : Science
ISBN : 9780128142110

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Introduction to Electrophysiological Methods and Instrumentation by Franklin Bretschneider,Jan R. de Weille Pdf

Introduction to Electrophysiological Methods and Instrumentation, Second Edition covers all topics of interest to electrophysiologists, neuroscientists and neurophysiologists, from the reliable penetration of cells and the behavior and function of the equipment, to the mathematical tools available for analyzing data. It discusses the pros and cons of techniques and methods used in electrophysiology and how to avoid pitfalls. Although the basics of electrophysiological techniques remain the principal purpose of this second edition, it now integrates several current developments, including, amongst others, automated recording for high throughput screening and multimodal recordings to correlate electrical activity with other physiological parameters collected by optical means. This book provides the electrophysiologist with the tools needed to understand his or her equipment and how to acquire and analyze low-voltage biological signals. Introduces possibilities and solutions, along with the problems, pitfalls, and artefacts of equipment and electrodes Discusses the particulars of recording from brain tissue slices, oocytes and planar bilayers Describes optical methods pertinent to electrophysiological practice Presents the fundamentals of signal processing of analogue signals, spike trains and single channel recordings, along with procedures for signal recording and processing Includes appendices on electrical safety and foundations of useful mathematical tools

Author : Ellen Covey,Matt Carter
Publisher : Oxford University Press
Page : 256 pages
File Size : 44,9 Mb
Release : 2015-02-25
Category : Medical
ISBN : 9780199342549

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Basic Electrophysiological Methods by Ellen Covey,Matt Carter Pdf

Basic Electrophysiological Methods provides a concise and easy-to-read guide on a selection of the most important contemporary electrophysiological techniques, their implementation, applications, and ways in which they can be combined and integrated with neuroscientific techniques. Intended for students, postdocs, and faculty with a basic neuroscience background, this text will not obscure the relevant technical details with textbook neuroscience tutorials as many other books do. Instead, each chapter provides a conscientious overview of the underlying theory -- a comprehensive description of equipment, materials, methods, data management, and analysis -- a troubleshooting guide, and a list of frequently asked questions. No book or online resource can function as strictly a DIY set of instructions on how to implement a complex technique. However, this book provides a fundamental and accessible set of information intended to form a foundation prior to, during, and after hands-on experience and training, greatly facilitating the initial learning process and subsequent fine-tuning of technical details.

Author : Helmut Kettenmann,Rosemarie Grantyn
Publisher : Wiley-Liss
Page : 480 pages
File Size : 48,5 Mb
Release : 1992-11-11
Category : Medical
ISBN : UOM:39015049078432

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Practical Electrophysiological Methods by Helmut Kettenmann,Rosemarie Grantyn Pdf

Designed to enable newcomers to electrophysiology to choose appropriate preparations and techniques for in vitro studies in vertebrate neurobiology. Emphasizes techniques involving the glass micropipette and other recently developed methods, supplementing easy-to-follow instructions with illustrative schemes, examples of original records, photomicrographs, key word listings, comprehensive reference tables and a list of suppliers and key equipment and reagents.

Author : Ellen Covey,Matt Carter
Publisher : Oxford University Press, USA
Page : 241 pages
File Size : 55,5 Mb
Release : 2015
Category : Medical
ISBN : 9780199939800

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Basic Electrophysiological Methods by Ellen Covey,Matt Carter Pdf

A broad, concise, and no-nonsense guide to contemporary electrophysiological techniques, covering intracellular and extracellular recording through recording of population activity, neuropharmacology, dye imaging, voltammetry, and optogenetics.

Author : William L. Nastuk
Publisher : Elsevier
Page : 442 pages
File Size : 51,6 Mb
Release : 2013-10-22
Category : Science
ISBN : 9781483194912

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Electrophysiological Methods by William L. Nastuk Pdf

Physical Techniques in Biological Research Volume VI: Electrophysiological Methods presents the analytical methods and experimental techniques in electrophysiological research. It discusses the handling and analysis of information by computer methods. It addresses the methods of analysis of waveforms, signal characterization and detection. Some of the topics covered in the book are the fundamentals of digital and analog computers; analysis of complex waveforms; operational amplifiers; signal processing and parameter estimation; the core conductor model; voltage clamp techniques; cable theory; automatic computation equipment; and electric accounting machinery. The wave shape generation are covered. The characterization of systematic functions is discussed. The text describes the nerve containing axial wire. A study of the internal thin and outer diffuse electrodes is presented. A chapter is devoted to the simple one dimensional model. Another section focuses on the cylindrical model with radial symmetry and accuracy of membrane potential measurement. The book can provide useful information to experimenters, students, and researchers.

Author : Jan Bures,Mojmir Petran,Jozef Zachar
Publisher : Academic Press
Page : 824 pages
File Size : 53,7 Mb
Release : 2013-10-22
Category : Science
ISBN : 9781483261454

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Electrophysiological Methods in Biological Research by Jan Bures,Mojmir Petran,Jozef Zachar Pdf

Electrophysiological Methods in Biological Research, Third Revised Edition describes the principles and applications of significant electrophysiological methods as regards to transistorisation of electrophysiological apparatus and to the mathematical analysis of electrophysiological data. The book explains the aspects of physics and electronics that are important in electrophysiology, such as the basic principles of semiconductor function, electronic simulators, electrodes, and the processing of electrophysiological data. The text also cites several examples that measure the resulting membrane potential if one electrode is inside the cell while the other is in contact with the cell's surface. Other experiments show the electrophysiological techniques and the fundamentals of electrical activity in the peripheral excitable structures, and its association with physiological functions. In considering the problems of nerve and muscle physiology, the investigator should know the technique of recording the electrical signs of a nerve impulse. These signs, or action potentials, indicate the presence of a nerve impulse. The text also discusses the effects of barbiturates or ether anesthesia in EEG activity, as well as its dissociation after physostigmine and atropine have been administered. The book can prove useful for pharmacologists, microchemists, cellular biologists, and research workers and technologists dealing with neural mechanisms.

Author : Jürgen Rettinger,Silvia Schwarz,Wolfgang Schwarz
Publisher : Springer Nature
Page : 226 pages
File Size : 52,7 Mb
Release : 2022-02-11
Category : Medical
ISBN : 9783030864828

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Electrophysiology by Jürgen Rettinger,Silvia Schwarz,Wolfgang Schwarz Pdf

This updated and revised textbook presents a broad overview on topics concerning cellular electrophysiology – ranging from bioelectric phenomena recognized as far back as ancient Egypt to popular topics on the dangers of electrosmog. Without sacrificing scientific precision, this clear and concise work presents on the one hand the different methods and modern applications, on the other hand the biophysical fundamentals of ion-channel and carrier proteins. Numerous and carefully selected illustrations and diagrams supplement the text. Each chapter is extended by a detailed table of Take-Home Messages. Questions at the end of each chapter allow readers to test their understanding. Each section also includes references to relevant original literature for further reading. The book offers a valuable resource for students of biology, chemistry and physics with a special interest in biophysics.

Author : Matt Carter,Rachel Essner,Nitsan Goldstein,Manasi Iyer
Publisher : Academic Press
Page : 416 pages
File Size : 43,8 Mb
Release : 2022-03-26
Category : Medical
ISBN : 9780323915618

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Guide to Research Techniques in Neuroscience by Matt Carter,Rachel Essner,Nitsan Goldstein,Manasi Iyer Pdf

Modern neuroscience research is inherently multidisciplinary, with a wide variety of cutting edge new techniques to explore multiple levels of investigation. This Third Edition of Guide to Research Techniques in Neuroscience provides a comprehensive overview of classical and cutting edge methods including their utility, limitations, and how data are presented in the literature. This book can be used as an introduction to neuroscience techniques for anyone new to the field or as a reference for any neuroscientist while reading papers or attending talks. Nearly 200 updated full-color illustrations to clearly convey the theory and practice of neuroscience methods Expands on techniques from previous editions and covers many new techniques including in vivo calcium imaging, fiber photometry, RNA-Seq, brain spheroids, CRISPR-Cas9 genome editing, and more Clear, straightforward explanations of each technique for anyone new to the field A broad scope of methods, from noninvasive brain imaging in human subjects, to electrophysiology in animal models, to recombinant DNA technology in test tubes, to transfection of neurons in cell culture Detailed recommendations on where to find protocols and other resources for specific techniques "Walk-through" boxes that guide readers through experiments step-by-step

Author : Daniel C. Sigg,Paul A. Iaizzo,Yong-Fu Xiao,Bin He
Publisher : Springer Science & Business Media
Page : 492 pages
File Size : 53,5 Mb
Release : 2010-09-11
Category : Medical
ISBN : 9781441966582

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Cardiac Electrophysiology Methods and Models by Daniel C. Sigg,Paul A. Iaizzo,Yong-Fu Xiao,Bin He Pdf

Cardiovascular disease is the major cause of mortality and morbidity in the Western Hemisphere. While significant progress has been made in treating a major sub-category of cardiac disease, arrhythmias, significant unmet needs remain. In particular, every day, thousands of patients die because of arrhythmias in the US alone, and atrial fibrillation is the most common arrhythmia affecting millions of patients in the US alone at a given time. Therefore, there is a public need to continue to develop new and better therapies for arrhythmias. Accordingly, an ever increasing number of biomedical, pharmaceutical, and medical personnel is interested in studying various aspects of arrhythmias at a basic, translational, and applied level, both in industry (ie Biotech, Pharmaceutical and device), and in academia. Not only has our overall understanding of molecular bases of disease dramatically increased, but so has the number of available and emerging molecular, pharmacological or device treatment based therapies. This practical, state-of-the art handbook will summarize and review key research methods and protocols, their advantages and pitfalls, with a focus on practical implementation, and collaborative cross-functional research. The volume will include visual and easy-to-use graphics, bulleted summaries, boxed summary paragraphs, links to reference websites, equipment manufacturers where appropriate, photographs of typical experimental setups and so forth, to keep this book very focused on practical methods and implementation, and yet, provide enough theory that the principles are clearly understood and can be easily applied.

Author : Robert P Vertes,Timothy Allen
Publisher : Springer Nature
Page : 256 pages
File Size : 45,5 Mb
Release : 2022-09-06
Category : Medical
ISBN : 9781071626313

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Electrophysiological Recording Techniques by Robert P Vertes,Timothy Allen Pdf

This second edition expands on the previous volume by incorporating state-of-the-art electrophysiological and anatomical methods and their application to the study of several systems of the brain involved in a range of functions. Chapters in this edition cover topics such as the value and difficulty of multi-site recordings using depth or surface electrodes; an assessment of different electrophysiological techniques used in non-human animals and humans; applying single-unit and ensemble recordings to the study of temporal dynamics in cognition; approaches to electroencephalography (EEG) recordings applied to mouse research; recordings of the nucleus reuniens of the ventral midline thalamus; and a comprehensive account of intracranial electroencephalography performed in patients with drug resistant epilepsy. In the Neuromethods series style, chapters include the type of detail and key advice from specialists needed to obtain successful results in your laboratory. Comprehensive and thorough, Electrophysiological Recording Techniques, Second Edition is a valuable resource for researchers and clinicians to help them utilize the principles and research designs described herein in their programs.

Author : Mark Dallas,Damian Bell
Publisher : Humana
Page : 333 pages
File Size : 43,7 Mb
Release : 2021-11-13
Category : Medical
ISBN : 1071608207

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Patch Clamp Electrophysiology by Mark Dallas,Damian Bell Pdf

This volume describes a range of standard and novel methodological approaches used to probe ion channel function across different modalities. Chapters guide readers through methods and protocols from an introduction to the decades old patch clamp method for the ion channel neophyte to more complex, recent protocol advances, such as optogenetics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Patch Clamp Electrophysiology: Methods and Protocols aims to be a reference guide for current and future ion channel physiologists.

Author : Gerald Oster,William L. Nastuk
Publisher : Academic Press
Page : 446 pages
File Size : 50,9 Mb
Release : 1963
Category : Science
ISBN : 0125141068

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Electrophysiological Methods by Gerald Oster,William L. Nastuk Pdf

Author : Juliusz Ashot Kozak,James W. Putney, Jr.
Publisher : CRC Press
Page : 343 pages
File Size : 45,6 Mb
Release : 2017-07-14
Category : Science
ISBN : 9781498752732

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Calcium Entry Channels in Non-Excitable Cells by Juliusz Ashot Kozak,James W. Putney, Jr. Pdf

Calcium Entry Channels in Non-Excitable Cells focuses on methods of investigating the structure and function of non-voltage gated calcium channels. Each chapter presents important discoveries in calcium entry pathways, specifically dealing with the molecular identification of store-operated calcium channels which were reviewed by earlier volumes in the Methods in Signal Transduction series. Crystallographic and pharmacological approaches to the study of calcium channels of epithelial cells are also discussed. Calcium ion is a messenger in most cell types. Whereas voltage gated calcium channels have been studied extensively, the non-voltage gated calcium entry channel genes have only been identified relatively recently. The book will fill this important niche.

Author : Jan Bureš,Mojmír Petráň,Jozef Zachar
Publisher : Unknown
Page : 524 pages
File Size : 51,6 Mb
Release : 1960
Category : Biologia
ISBN : UOM:39015009530356

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Electrophysiological Methods in Biological Research by Jan Bureš,Mojmír Petráň,Jozef Zachar Pdf

Rozsáhlá práce s přehledem teorie, elektrofyziologických přístrojů a techniky má jednotlivé kapitoly zpracovány jako monografická pojednání o elektrofyziologii buněk a tkání, izolovaných struktur in vitro,periferníchstrukturinsitu, receptorů, míchy, mozkové kůry a podkorových struktur. Dílo je doplněno atlasem pro stereotaxi kočky, králíka a krysy.

Author : Alexander G. Volkov
Publisher : Springer Science & Business Media
Page : 513 pages
File Size : 49,5 Mb
Release : 2007-04-19
Category : Science
ISBN : 9783540378433

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Plant Electrophysiology by Alexander G. Volkov Pdf

This book compiles new findings in plant electrophysiology from the work of internationally renowned experts in the fields of electrophysiology, bio-electrochemistry, biophysics, signal transduction, phloem transport, tropisms, ion channels, plant electrochemistry, and membrane transport. Opening with a historical introduction, the book reviews methods in plant electrophysiology, introducing such topics as measuring membrane potentials and ion fluxes, path-clamp technique, and electrochemical sensors. The coverage includes experimental results and their theoretical interpretation.