Excitable Cells In Tissue Culture

Author : Phillip G. Nelson,Melvyn Lieberman
Publisher : Springer Science & Business Media
Page : 431 pages
File Size : 53,6 Mb
Release : 2012-12-06
Category : Science
ISBN : 9781468438031

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Excitable Cells in Tissue Culture by Phillip G. Nelson,Melvyn Lieberman Pdf

The tissue culture approach to the study of membrane properties of excitable cells has progressed beyond the technical problems of culture methodology. Recent developments have fostered substantive contributions in research con cerned with the physiology, pharmacology, and biophysics of cell membranes in tissue culture. The scope of this volume is related to the application of tissue culture methodology to developmental processes and cellular mechanisms of electrical and chemical excitability. The major emphasis will be on the body of new biological information made available by the analytic possibilities inherent in the tissue culture systems. Naturally occurring preparations of excitable cells are frequently of suf ficient morphological complexity to compromise the analysis of the data obtained from them. Some of the limitations associated with dissected prepa rations have to do with the direct visualization of and access to the cell(s) in question and maintenance of steady-state conditions for prolonged periods of time. Since preparations in tissue culture can circumvent these problems, it is feasible to analyze the properties of identifiable cells, grown either singly or in prescribed geometries, as well as to follow the development of cellular inter actions. A crucial consideration in the use of cultured preparations is that they must faithfully capture the phenomenon of interest to the investigator. This and other potential limitations on the methodology are of necessary concern in the present volume.

Author : 3Island Press
Publisher : Unknown
Page : 444 pages
File Size : 47,9 Mb
Release : 1981-05-31
Category : Electronic
ISBN : 1468438042

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Excitable Cells in Tissue Culture by 3Island Press Pdf

Author : Alan L. Harvey
Publisher : Springer Science & Business Media
Page : 317 pages
File Size : 48,5 Mb
Release : 2012-12-06
Category : Medical
ISBN : 9781468488104

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The Pharmacology of Nerve and Muscle in Tissue Culture by Alan L. Harvey Pdf

The techniques of tissue culture were introduced at the beginning of this century. They have become more and more popular as it is realized that they are not as difficult or as esoteric as some early protagonists liked to maintain. Most of the work performed with culture methods has simply concerned cell growth and survival. Biologists have long used culture approaches to provide a simple system in which to study cell division and multiplication. Any pharmacology done on cultured tissue was largely toxicological or as part of a screening programme for poten tial anti-cancer drugs. In the last decade there has been a great increase in the use of excitable cells in tissue culture. Nerves and muscles from a wide variety of sources can maintain their highly differentiated properties in culture. Such cultures offer an attractive preparation for use in physiological and pharmacological investigations. Consequently, a vast amount of work has been produced, and this book is an attempt to review it. It is hoped that this will introduce physiologists and pharmacologists to the potential of culture methods for their experiments and also indicate to more traditional tissue culture users further possible areas of interest. By being more comprehensive in scope and by trying to concentrate largely on drug actions, I hope that the present volume usefully extends the treatment of the subject begun earlier in the excellent works by Crain (1976) and Nelson and Lieberman (1981).

Author : Juliusz Ashot Kozak,James W. Putney, Jr.
Publisher : CRC Press
Page : 343 pages
File Size : 48,6 Mb
Release : 2017-07-14
Category : Science
ISBN : 9781498752732

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Calcium Entry Channels in Non-Excitable Cells by Juliusz Ashot Kozak,James W. Putney, Jr. Pdf

Calcium Entry Channels in Non-Excitable Cells focuses on methods of investigating the structure and function of non-voltage gated calcium channels. Each chapter presents important discoveries in calcium entry pathways, specifically dealing with the molecular identification of store-operated calcium channels which were reviewed by earlier volumes in the Methods in Signal Transduction series. Crystallographic and pharmacological approaches to the study of calcium channels of epithelial cells are also discussed. Calcium ion is a messenger in most cell types. Whereas voltage gated calcium channels have been studied extensively, the non-voltage gated calcium entry channel genes have only been identified relatively recently. The book will fill this important niche.

Author : Donald Cheng
Publisher : Unknown
Page : 516 pages
File Size : 48,7 Mb
Release : 2014-01-15
Category : Electronic
ISBN : 1461593387

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Structure and Function in Excitable Cells by Donald Cheng Pdf

Author : Toshio Narahashi
Publisher : Academic Press
Page : 402 pages
File Size : 47,7 Mb
Release : 2013-10-22
Category : Medical
ISBN : 9781483288338

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Ion Channels of Excitable Cells by Toshio Narahashi Pdf

Because of the highly significant and widely recognized roles of ion channels in physiology, pathophysiology, pharmacology, and toxicology, the term ion channel has now become a household word in the biomedical sciences. This volume covers preparations and techniques for the study of various ion channels. Both voltage-gated and ligand-gated ion channels of neurons, axons, and cardiac and smooth muscles are covered. It includes not only patch clamp techniques but molecular biology and imaging techniques as well. Comprehensive protocols included for the study of: Ion channels using patch-clamp, molecular biology, and imaging techniques Role of ion channels in physiology, pathophysiology, pharmacology, and toxicology Specific ion channels of specific tissues

Author : Bruce Alberts
Publisher : Unknown
Page : 0 pages
File Size : 46,7 Mb
Release : 2002
Category : Cytology
ISBN : 0815332181

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Molecular Biology of The Cell by Bruce Alberts Pdf

Author : Bertil Hille
Publisher : Sinauer Associates, Incorporated
Page : 632 pages
File Size : 47,9 Mb
Release : 1992
Category : Medical
ISBN : UOM:39015024124383

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Ionic Channels of Excitable Membranes by Bertil Hille Pdf

This new, fully revised and expanded edition of Ionic Channels of Excitable Membranes includes new chapters on fast chemical synapses, modulation through G protein coupled receptors and second messenger systems, molecules cloning, site directed mutagenesis, and cell biology. It begins with the classical biophysical work of Hodgkin and Huxley and then weaves a description of the known ionic channels together with their biological functions. The book continues by developing the physical and molecular principles needed for explaining permeation, gating, pharmacological modification, and molecular diversity, and ends with a discussion of channel evolution. Ionic Channels of Excitable Membranes is written to be accessible and interesting to biological and physical scientists of all kinds.

Author : Bert Sakmann
Publisher : Springer Science & Business Media
Page : 506 pages
File Size : 43,9 Mb
Release : 2013-11-11
Category : Medical
ISBN : 9781461578581

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Single-Channel Recording by Bert Sakmann Pdf

Single-channel recording has become a widely used tool for the study of ion permeation mechanisms in biological membranes. Whereas the technique might have been considered an "art" after its introduction in 1976, it developed into a relatively simple method after it became possible to obtain high-resistance (several gigaohm) membrane-pipette seals. In the summer of 1982, a course on the technique was held at the Ettore Majorana Center for Scientific Culture in Erice, Sicily. It brought together people from most of the laboratories involved in patch clamping at that time. During the course, it became apparent that the technique had reached a state of maturity. Repeatedly, the opinion was expressed that a detailed description of all the aspects of the technique including representative examples of results should be available. We therefore asked the course instructors, as well as several other colleagues, to provide chapters on selected topics in order to produce this volume. The different variants of patch clamping were described quite extensively in an article by Hamill, Marty, Neher, Sakmann, and Sigworth (Pflugers Archiv 391:85) in 1981. Rather than repeating this survey in an introductory chapter, we chose to reprint that article in the Appendix of this volume (by permission of Springer-Verlag). The methods section will, therefore, go straight into detailed aspects of the technol ogy.

Author : Sergey Fedoroff,Arleen Richardson
Publisher : Springer Science & Business Media
Page : 284 pages
File Size : 52,7 Mb
Release : 2013-04-17
Category : Medical
ISBN : 9781475725865

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Protocols for Neural Cell Culture by Sergey Fedoroff,Arleen Richardson Pdf

This volume is designed to be kept close at hand as a ready reference and a guide to laboratory procedures. It is based on tissue culture manuals used for a number of years at international courses on tissue culture at the University of Saskatchewan, made possible by the generous support of the Canadian Council of Animal Care and the Medical Research Council of Canada. Sergey Fedoroff Arleen Richardson The second edition of Protocols for Neural Cell Culture adheres to the prin ciples enunciated in the first edition, but the content has been extensively revised and expanded. Two new chapters have been added to reflect the increased interest in the development and differentiation of the nervous system and in the reconstruction of its circuitry in tissue culture. One chapter deals with slice cul tures in which the organization of the nervous system is preserved. When slice cultures are combined with explant cultures, afferent and efferent projections can be reconstructed. The other chapter deals with aggregating neural cell cul tures, in which "minibrains" can form. Theses are small, uniformly sized spheres of nervous tissue, usually having nerve cells in the center and astrocytes, oligo dendrocytes, and microglia in the periphery. Such cultures can be used to study neutral cell interactions in an organized milieu and for qualitative as well as quantitative studies at biochemical and molecular levels.

Author : Edouard Kurstak,Z.J. Lipowski,P.V. Morozov
Publisher : Springer
Page : 457 pages
File Size : 50,9 Mb
Release : 2014-01-13
Category : Medical
ISBN : 9781461317999

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Viruses, Immunity, and Mental Disorders by Edouard Kurstak,Z.J. Lipowski,P.V. Morozov Pdf

In spite of progress in biomedical research, we know little about the causes, prevention, and treatment of the numerous mental and neurological disorders that afflict up to 15% of all individuals. In the last decade, great advances have been made in the physiopathology of mental and neurological disorders, leading to at least a partial control of Parkinson's disease, epilepsy, certain psychoses, and anxiety syndromes. Despite the fact that an underlying specific neurotransmitter deficiency has been demonstrated in Alzheimer's and Parkinson's diseases, the immune dysfunction and viral hypotheses continue to be attractive for investigators dealing with these degenerative diseases of the aging brain, which afflict 10% of senior citizens. A retrospective epidemiologic study suggests that the encephalitis lethargica and parkinsonism were almost certainly caused by the 1918 influenza virus pandemics. It must be stressed that the etiopathogenesis of many mental disorders is not known, and this ignorance has led to several untenable neurophysiological and biochemical hypotheses. Epidemiologic investigations show a high prevalence of functional psychoses and organic mental disorders. Although many of them are conceptualized as biopsychosocial disorders, recent data indicate that the biological component appears more and more as a major etiologic factor. Among the various biological hypotheses, the viral and im munologic concept has become a significant one. In view of recent discoveries in virology and immunity, it becomes clear that viral and immunologic hypotheses should be inves tigated more systematically concerning the mechanisms of numerous mental and neu rological disorders.

Author : Mohammad M. Aria
Publisher : Academic Press
Page : 180 pages
File Size : 49,5 Mb
Release : 2020-05-15
Category : Medical
ISBN : 9780128170717

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Electrophysiology Measurements for Studying Neural Interfaces by Mohammad M. Aria Pdf

Electrophysiology Measurements for Studying Neural Interfaces helps readers to choose a proper cell line and set-up for studying different bio-electronic interfaces before delving into the electrophysiology techniques available. Therefore, this book details the materials and devices needed for different types of neural stimulation such as photoelectrical and photothermal stimulations. Also, modern techniques like optical electrophysiology and calcium imaging in this book provides readers with more available approaches to monitor neural activities in addition to whole-cell patch-clamp technology. Details steps of an electrophysiology project from start to finish for graduate students employing the technique in their research Includes sample electrophysiological studies with multiple cell lines (PC12, N2a, NG108, SHSY, and embryonic stem cell lines) to facilitate research Features data analysis of electrophysiology results from various relevant experiments and cell culture tips

Author : Anonim
Publisher : Academic Press
Page : 340 pages
File Size : 52,9 Mb
Release : 1988-02-01
Category : Science
ISBN : 0080585027

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Current Topics in Membranes and Transport by Anonim Pdf

Current Topics in Membranes and Transport

Author : Anonim
Publisher : Unknown
Page : 1230 pages
File Size : 51,8 Mb
Release : 1980
Category : Medicine
ISBN : MINN:30000008789954

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Research Awards Index by Anonim Pdf

Author : Gordon Sato
Publisher : Springer Science & Business Media
Page : 298 pages
File Size : 44,8 Mb
Release : 2013-06-29
Category : Medical
ISBN : 9781468429046

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Tissue Culture of the Nervous System by Gordon Sato Pdf

The impetus for compiling this book was the recent development of culture strains of neuroblastoma and glial cells and the immediate and enthusiastic way they have been taken up as model systems. After the first sudden rush of activity, it seems appropriate to pause, to assess progress, and to contemplate the future contributions that may be possible using these culture techniques. Long before the advent of established strains, cultures of nervous tissue had already contributed to neurobiology. Ross Harrison, in 1906, in a single experimental series, established tissue culture as a promising new technique in cell biology and settled the Golgi-Cajal controversy as to whether axonic processes originated as outgrowths from the cell body or were formed first in the intercellular spaces and were later connected to the cell body. Harrison observed process growth from nerve cells in cultures, thus settling the matter in favor of Cajal. Of great importance to neurobiology is the discovery by Rita Levi-Montalcini of nerve growth factor. Cultures of spinal ganglia played a major role in the discovery, isolation, and characterization of the factor (Levi-Montalcini et ai. , 1954). In my opinion, this discovery, although very well known, has not yet been adequately recognized for its germinal influence on neurobiology and embryology. Progress since the advent of clonal cultures has been more modest. I would like to cite two pieces of work which emphasize the technical ad vantages of these cultures.