Gus Protocols

Author : Sean R. Gallagher
Publisher : Academic Press
Page : 221 pages
File Size : 41,5 Mb
Release : 2012-12-02
Category : Science
ISBN : 9780323137645

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GUS Protocols by Sean R. Gallagher Pdf

The gb-glucuronidase (GUS) gene is extremely useful as a reporter of the expression of introduced genes and can be used in organisms where other reporter genes are useless. Thus, the GUS gene is the reporter gene of choice for transgenic plant research. Not only can this assay be used to detect whether a gene is being expressed, but it can be used to determine the location of the gene product within cells. Low cost is another advantage of this assay. GUS Protocols provides instructions and essential background information that will enable researchers to effectively use the GUS gene as a reporter of the expression of introduced genes. First book on the GUS reporter system Up-to-date reference lists following each of the fourteen chapters Comb-bound for convenient bench-top use Written by leading authorities including R.A. Jefferson, inventor of the GUS assay Illustrated with color GUS detection by fluorometric, spectrophotometric, and histochemical methods Fast, automated assays

Author : Julio Salinas,Jose J. Sanchez-Serrano
Publisher : Springer Science & Business Media
Page : 461 pages
File Size : 46,7 Mb
Release : 2008-02-04
Category : Nature
ISBN : 9781597450034

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Arabidopsis Protocols, 2nd Edition by Julio Salinas,Jose J. Sanchez-Serrano Pdf

For several decades, Arabidopsis thaliana has been the organism of choice in the laboratories of many plant geneticists, physiologists, developmental biologists, and biochemists around the world. During this time, a huge amount of knowledge has been acquired on the biology of this plant species, which has resulted in the development of molecular tools that account for much more efficient research. The significance that Arabidopsis would attain in biological research may have been difficult to foresee in the 1980s, when its use in the laboratory started. In the meantime, it has become the model plant organism, much the same way as Drosophila, Caenorhabditis, or mouse have for animal systems. Today, it is difficult to envision research at the cutting edge of plant biology without the use of Arabidopsis. Since the first edition of Arabidopsis Protocols appeared, new developments have fostered an impressive advance in plant biology that prompted us to prepare Arabidopsis Protocols, Second Edition. Completion of the Arabidopsis genome sequence offered for the first time the opportunity to have in hand all of the genetic information required for studying plant function. In addition, the development of whole systems approaches that allow global analysis of gene expression and protein and metabolite dynamics has encouraged scientists to explore new scenarios that are extending the limits of our knowledge.

Author : Gary D. Foster,Sally Taylor
Publisher : Springer Science & Business Media
Page : 557 pages
File Size : 45,7 Mb
Release : 2008-02-03
Category : Science
ISBN : 9781592595662

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Plant Virology Protocols by Gary D. Foster,Sally Taylor Pdf

The aim of Plant Virology Protocols is to provide a source of infor- tion to guide the reader through the wide range of methods involved in gen- ating transgenic plants that are resistant to plant viruses. To this end, we have commissioned a wide-ranging list of chapters that will cover the methods required for: plant virus isolation; RNA extraction; cloning coat p- tein genes; introduction of the coat protein gene into the plant genome; and testing transgenic plants for resistance. The book then moves on to treatments of the mechanisms of resistance, the problems encountered with field testing, and key ethical issues surrounding transgenic technology. Although Plant Virology Protocols deals with the cloning and expression of the coat protein gene, the techniques described can be equally applied to other viral genes and nucleotide sequences, many of which have also been shown to afford protection when introduced into plants. The coat protein has, however, been the most widely applied, and as such has been selected to illustrate the techniques involved. Plant Virology Protocols has been divided into six major sections, c- taining 55 chapters in total.

Author : Kan Wang
Publisher : Springer Science & Business Media
Page : 473 pages
File Size : 41,5 Mb
Release : 2008-02-05
Category : Science
ISBN : 9781597451314

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Agrobacterium Protocols by Kan Wang Pdf

Agrobacterium tumefaciens is a soil bacterium that for more than a century has been known as a pathogen causing the plant crown gall disease. Unlike many other pathogens, Agrobacterium has the ability to deliver DNA to plant cells and permanently alter the plant genome. The discovery of this unique feature 30 years ago has provided plant scientists with a powerful tool to genetically transform plants for both basic research purposes and for agric- tural development. Compared to physical transformation methods such as particle bomba- ment or electroporation, Agrobacterium-mediated DNA delivery has a number of advantages. One of the features is its propensity to generate single or a low copy number of integrated transgenes with defined ends. Integration of a single transgene copy into the plant genome is less likely to trigger “gene silencing” often associated with multiple gene insertions. When the first edition of Agrobacterium Protocols was published in 1995, only a handful of plants could be routinely transformed using Agrobacterium. Ag- bacterium-mediated transformation is now commonly used to introduce DNA into many plant species, including monocotyledon crop species that were previously considered non-hosts for Agrobacterium. Most remarkable are recent devel- ments indicating that Agrobacterium can also be used to deliver DNA to non-plant species including bacteria, fungi, and even mammalian cells.

Author : Shri Mohan Jain,Pramod K. Gupta
Publisher : Springer Science & Business Media
Page : 604 pages
File Size : 40,8 Mb
Release : 2005-05-23
Category : Science
ISBN : 1402029845

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Protocol for Somatic Embryogenesis in Woody Plants by Shri Mohan Jain,Pramod K. Gupta Pdf

World population is increasing at an alarming rate and this has resulted in increasing tremendously the demand for tree products such as wood for construction materials, fuel and paper, fruits, oils and medicines etc. This has put immense pressure on the world’s supplies of trees and raw material to industry and will continue to do so as long as human population continues to grow. Also, the quality of human diet, especially nutritional components, is adversely affected due to limited genetic improvement of most of fruit trees. Thus there is an immediate need to increase productivity of trees. Improvement has been made through conventional breeding methods, however, conventional breeding is very slow due to long life cycle of trees. A basic strategy in tree improvement is to capture genetic gain through clonal propagation. Clonal propagation via organogenesis is being used for the production of selected elite individual trees. However, the methods are labour intensive, costly, and produce low volumes. Genetic gain can now be captured through somatic embryogenesis. Formation of embryos from somatic cells by a process resembling zygotic embryogenesis is one of the most important features of plants. In 1958, Reinert in Germany and Steward in USA independently reported somatic embryogenesis in carrot cultures. Since then, tremendous progress in somatic embryogenesis of woody and non-woody plants has taken place. It offers a potentially large-scale propagation system for superior clones.

Author : Prakash Singh Bisen
Publisher : CRC Press
Page : 1812 pages
File Size : 54,5 Mb
Release : 2014-02-26
Category : Medical
ISBN : 9781466553149

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Laboratory Protocols in Applied Life Sciences by Prakash Singh Bisen Pdf

As applied life science progresses, becoming fully integrated into the biological, chemical, and engineering sciences, there is a growing need for expanding life sciences research techniques. Anticipating the demands of various life science disciplines, Laboratory Protocols in Applied Life Sciences explores this development. This book covers a wide spectrum of areas in the interdisciplinary fields of life sciences, pharmacy, medical and paramedical sciences, and biotechnology. It examines the principles, concepts, and every aspect of applicable techniques in these areas. Covering elementary concepts to advanced research techniques, the text analyzes data through experimentation and explains the theory behind each exercise. It presents each experiment with an introduction to the topic, concise objectives, and a list of necessary materials and reagents, and introduces step-by-step, readily feasible laboratory protocols. Focusing on the chemical characteristics of enzymes, metabolic processes, product and raw materials, and on the basic mechanisms and analytical techniques involved in life science technological transformations, this text provides information on the biological characteristics of living cells of different origin and the development of new life forms by genetic engineering techniques. It also examines product development using biological systems, including pharmaceutical, food, and beverage industries. Laboratory Protocols in Applied Life Sciences presents a nonmathematical account of the underlying principles of a variety of experimental techniques in disciplines, including: Biotechnology Analytical biochemistry Clinical biochemistry Biophysics Molecular biology Genetic engineering Bioprocess technology Industrial processes Animal Plant Microbial biology Computational biology Biosensors Each chapter is self-contained and written in a style that helps students progress from basic to advanced techniques, and eventually design and execute their own experiments in a given field of biology.

Author : Edward Chee Tak Yeung,Claudio Stasolla,Michael John Sumner,Bing Quan Huang
Publisher : Springer
Page : 576 pages
File Size : 44,5 Mb
Release : 2015-09-19
Category : Science
ISBN : 9783319199443

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Plant Microtechniques and Protocols by Edward Chee Tak Yeung,Claudio Stasolla,Michael John Sumner,Bing Quan Huang Pdf

A proper understanding of the structural organization of the plant body is essential to any study in plant biology. Experimental studies in vivo and in situ will lead to structural, physiological, and cellular changes of the experimental material. To study macroscopic and microscopic changes, different histological methods and microtechniques can be used as they provide valuable information of the experimental system. In addition, the observed structural changes allow investigators to set hypothesis for further studies based on one’s own observation. Thus, proper selection and utilization of microtechniques are a must for the success of a research program. At present, an up-to-date collection of protocols are not readily available in the literature. The latest work in plant microtechniques was published in 1999 by Ruzin but many others are no longer in print [e.g., Jensen (1964); O’Brien and McCully (1981)]. Furthermore, a majority of published works focus on techniques related to general processing and staining procedures. A comprehensive treatment that encompasses broader applications of microtechniques to other disciplines is lacking [e.g., archeology, wood science, etc.]. There is a need to create a comprehensive volume of botanical methods and protocols which includes traditional and novel techniques that can be used by researchers in plant science and investigators in other disciplines that require plant microtechniques in their research and teaching. This book covers a wide variety of applications and brings them up-to-date to make them understandable and relevant, especially to students using the methods for the first time. It is our intention to create a useful reference for plant histology and related methods that will serve as a foundation for plant scholars, researchers, and teachers in the plant sciences.​

Author : John Flex Jackson,Hans F. Linskens
Publisher : Springer Science & Business Media
Page : 194 pages
File Size : 42,5 Mb
Release : 2013-03-09
Category : Science
ISBN : 9783662049044

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Testing for Genetic Manipulation in Plants by John Flex Jackson,Hans F. Linskens Pdf

Molecular Methods of Plant Analysis Concept of the Series The powerful recombinant DNA technology and related developments have had an enormous impact on molecular biology. Any treatment of plant analy sis must make use of these new methods. Developments have been so fast and the methods so powerful that the editors of Modern Methods of Plant Analy sis have now decided to rename the series Molecular Methods of Plant Analy sis. This will not change the general aims of the series, but best describes the thrust and content of the series as we go forward into the new millennium. This does not mean that all chapters a priori deal only with the methods of molecular biology, but rather that these methods are to be found in many chapters together with the more traditional methods of analysis which have seen recent advances. The numbering of the volumes of the series therefore continues on from 20, which is the most recently published volume under the title Modern Methods of Plant Analysis. As indicated for previous volumes, the methods to be found in Molecular Methods of Plant Analysis are described critically, with hints as to their limi tations, references to original papers and authors being given, and the chap ters written so that there is little need to consult other texts to carry out the All authors have been chosen because of their methods of analysis described.

Author : Anonim
Publisher : Elsevier
Page : 651 pages
File Size : 49,8 Mb
Release : 2000-10-11
Category : Science
ISBN : 9780080496818

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Applications of Chimeric Genes and Hybrid Proteins, Part A: Gene Expression and Protein Purification by Anonim Pdf

The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.

Author : Anonim
Publisher : Unknown
Page : 172 pages
File Size : 47,9 Mb
Release : 1991
Category : Aflatoxins
ISBN : MINN:31951D02019931D

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Aflatoxin Elimination Workshop by Anonim Pdf

Author : Jac A. Nickoloff
Publisher : Springer Science & Business Media
Page : 209 pages
File Size : 52,9 Mb
Release : 2008-02-02
Category : Science
ISBN : 9781592595426

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Plant Cell Electroporation And Electrofusion Protocols by Jac A. Nickoloff Pdf

Gene transfer is an essential technology for improving our under standing of gene structure and function. Although there are many meth ods by which DNA may be introduced into cells—including heat and chemical treatments, and microinjection—electroporation has been found to be the most versatile gene transfer technique. Electroporation is effective with a wide variety of cell types, including those that are difficult to transform by other means. For many cell types, electroporation is either the most efficient or the only means known to effect gene transfer. The early and broad success of electric field-medi ated DNA transfer soon prompted researchers to investigate electroporation for transferring other types of molecules into cells, in cluding RNA, enzymes, antibodies, and analytic dyes. The first section of Plant Cell Electroporation and Electrofusion Protocols includes two chapters that serve as a guide to theoretical and practical aspects of electroporation, and will be of particular interest to those developing protocols for as yet untested species or cell types, and a third chapter that describes commercially available electroporation instruments. The remaining chapters describe well-tested protocols for DNA electrotransfection, electroporation of other biomolecules, or cell electrofusion. These chapters also include brief discussions of alterna tives to electric field-based methods, citing the advantages and limita tions of the various methods for achieving specific goals.

Author : Anonim
Publisher : Academic Press
Page : 383 pages
File Size : 43,9 Mb
Release : 2012-12-02
Category : Science
ISBN : 9780323140256

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Transgenic Plants by Anonim Pdf

Volumes 1 and 2 of Transgenic Plants assemble important information on transgenic crops which has appeared scattered in many different publications. These two volumes are a significant milestone in plant/agricultural biology, promote the practical application of recombinant DNA technology, and assist in transforming the agricultural industry.

Author : Arie Altman
Publisher : CRC Press
Page : 798 pages
File Size : 49,6 Mb
Release : 1997-11-06
Category : Technology & Engineering
ISBN : 1420049275

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Agricultural Biotechnology by Arie Altman Pdf

This work integrates basic biotechnological methodologies with up-to-date agricultural practices, offering solutions to specific agricultural needs and problems from plant and crop yield to animal husbandry. It presents and evaluates the limitations of classical methodologies and the potential of novel and emergent agriculturally related biotechnologies.

Author : Robert N. Trigiano
Publisher : Routledge
Page : 470 pages
File Size : 50,7 Mb
Release : 2018-04-27
Category : Science
ISBN : 9781351424035

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Plant Tissue Culture Concepts and Laboratory Exercises by Robert N. Trigiano Pdf

Alternating between topic discussions and hands-on laboratory experiments that range from the in vitro flowering of roses to tissue culture of ferns, Plant Tissue Culture Concepts and Laboratory Exercises, Second Edition, addresses the most current principles and methods in plant tissue culture research. The editors use the expertise of some of the top researchers and educators in plant biotechnology to furnish students, instructors and researchers with a broad consideration of the field. Divided into eight major parts, the text covers everything from the history of plant tissue culture and basic methods to propagation techniques, crop improvement procedures, specialized applications and nutrition of callus cultures. New topic discussions and laboratory exercises in the Second Edition include ""Micropropagation of Dieffenbachia,"" ""Micropropagation and in vitro flowering of rose,"" ""Propagation from nonmeristematic tissue-organogenesis,"" ""Variation in culture"" and ""Tissue culture of ferns.""It is the book's extensive laboratory exercises that provide a hands-on approach in illustrating various topics of discussion, featuring step-by-step procedures, anticipated results, and a list of materials needed. What's more, editors Trigiano and Gray go beyond mere basic principles of plant tissue culture by including chapters on genetic transformation techniques, and photographic methods and statistical analysis of data. In all, Plant Tissue Culture Concepts and Laboratory Exercises, Second Edition, is a veritable harvest of information for the continued study and research in plant tissue culture science.

Author : Paulo Veríssimo,Luís Rodrigues
Publisher : Springer Science & Business Media
Page : 625 pages
File Size : 41,6 Mb
Release : 2012-12-06
Category : Computers
ISBN : 9781461516637

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Distributed Systems for System Architects by Paulo Veríssimo,Luís Rodrigues Pdf

The primary audience for this book are advanced undergraduate students and graduate students. Computer architecture, as it happened in other fields such as electronics, evolved from the small to the large, that is, it left the realm of low-level hardware constructs, and gained new dimensions, as distributed systems became the keyword for system implementation. As such, the system architect, today, assembles pieces of hardware that are at least as large as a computer or a network router or a LAN hub, and assigns pieces of software that are self-contained, such as client or server programs, Java applets or pro tocol modules, to those hardware components. The freedom she/he now has, is tremendously challenging. The problems alas, have increased too. What was before mastered and tested carefully before a fully-fledged mainframe or a closely-coupled computer cluster came out on the market, is today left to the responsibility of computer engineers and scientists invested in the role of system architects, who fulfil this role on behalf of software vendors and in tegrators, add-value system developers, R&D institutes, and final users. As system complexity, size and diversity grow, so increases the probability of in consistency, unreliability, non responsiveness and insecurity, not to mention the management overhead. What System Architects Need to Know The insight such an architect must have includes but goes well beyond, the functional properties of distributed systems.