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Author : Charles E. Barker
Publisher : Unknown
Page : 0 pages
File Size : 41,7 Mb
Release : 1991
Category : Luminescence
ISBN : 0608219061
Author : Charles Edward Barker,Otto C. Kopp
Publisher : Unknown
Page : 208 pages
File Size : 54,6 Mb
Release : 1991
Category : Science
ISBN : STANFORD:36105031386761
Author : Anonim
Publisher : Unknown
Page : 0 pages
File Size : 50,6 Mb
Release : 1991
Category : Electronic
ISBN : OCLC:716410027
Author : Yves Engelborghs,Antonie J. W. G. Visser
Publisher : Unknown
Page : 816 pages
File Size : 50,6 Mb
Release : 2014
Category : Fluorescence microscopy
ISBN : 1627036490
Reflecting the expanding field's need for reliable protocols, Fluorescence Spectroscopy and Microscopy: Methods and Protocols offers techniques from a worldwide team of experts on this versatile and vital subject. The topics covered fall into four broad categories: steady-state fluorescence spectroscopy, time-resolved fluorescence spectroscopy, fluorescent probe development, and the various sub-categories of fluorescence microscopy, such as fluorescence recovery after photobleaching (FRAP), live cell FRET imaging (FRETim), fluorescence lifetime imaging (FLIM), fluorescence fluctuation spectroscopy (FFS), and single-molecule fluorescence spectroscopy (smFS). Written as a part of the popular Methods in Molecular Biology series, chapters include the kind of unambiguous detail and key implementation advice that proves essential for successful results.
Author : Anonim
Publisher : Academic Press
Page : 502 pages
File Size : 48,9 Mb
Release : 1989-04-01
Category : Science
ISBN : 0080859283
Fluorescence Microscopy of Living Cells in Culture, Part B
Author : Laura Marcu,Paul M. W. French,Daniel S. Elson
Publisher : CRC Press
Page : 574 pages
File Size : 49,6 Mb
Release : 2014-07-17
Category : Technology & Engineering
ISBN : 9781439861677
During the past two decades, there has been an increasing appreciation of the significant value that lifetime-based techniques can add to biomedical studies and applications of fluorescence. Bringing together perspectives of different research communities, Fluorescence Lifetime Spectroscopy and Imaging: Principles and Applications in Biomedical Diagnostics explores the remarkable advances in time-resolved fluorescence techniques and their role in a wide range of biological and clinical applications. Broadly accessible, the book captures the state-of-the-art of fluorescence lifetime metrology and imaging and provides current perspectives on their applications to biomedical studies of intact tissues and medical diagnosis. The text introduces these techniques within the wider context of fluorescence spectroscopy and describes basic principles underlying current instrumentation for fluorescence lifetime imaging and metrology (FLIM). It also covers the wide range of methods, including single channel (point) spectroscopy, fluorescence lifetime imaging microscopy, and single- and multi-photon excitation. Edited by pioneers in this field, with contributions from leading experts, the book includes an overview of complementary techniques that help researchers beginning FLIM research. It offers a comprehensive treatment of fundamental principles, instrumentation, analytical methods, and applications. It also provides an overview of the label-free contrast available from lifetime measurements of tissue autofluorescence and the prospects for exploiting this for clinical applications and biomedical research including drug discovery.
Author : Yves Engelborghs,Antonie J.W.G. Visser
Publisher : Humana
Page : 0 pages
File Size : 45,9 Mb
Release : 2016-08-23
Category : Medical
ISBN : 1493963155
Reflecting the expanding field’s need for reliable protocols, Fluorescence Spectroscopy and Microscopy: Methods and Protocols offers techniques from a worldwide team of experts on this versatile and vital subject. The topics covered fall into four broad categories: steady-state fluorescence spectroscopy, time-resolved fluorescence spectroscopy, fluorescent probe development, and the various sub-categories of fluorescence microscopy, such as fluorescence recovery after photobleaching (FRAP), live cell FRET imaging (FRETim), fluorescence lifetime imaging (FLIM), fluorescence fluctuation spectroscopy (FFS), and single-molecule fluorescence spectroscopy (smFS). Written as a part of the popular Methods in Molecular Biology series, chapters include the kind of unambiguous detail and key implementation advice that proves essential for successful results. Comprehensive and practical, Fluorescence Spectroscopy and Microscopy: Methods and Protocols aims to guide both ‘novice’ and established scientists toward furthering their research with these invaluable techniques.
Author : Joseph R. Lakowicz
Publisher : Springer Science & Business Media
Page : 487 pages
File Size : 45,7 Mb
Release : 2013-11-11
Category : Science
ISBN : 9781461576587
Fluorescence methods are being used increasingly in biochemical, medical, and chemical research. This is because of the inherent sensitivity of this technique. and the favorable time scale of the phenomenon of fluorescence. 8 Fluorescence emission occurs about 10- sec (10 nsec) after light absorp tion. During this period of time a wide range of molecular processes can occur, and these can effect the spectral characteristics of the fluorescent compound. This combination of sensitivity and a favorable time scale allows fluorescence methods to be generally useful for studies of proteins and membranes and their interactions with other macromolecules. This book describes the fundamental aspects of fluorescence. and the biochemical applications of this methodology. Each chapter starts with the -theoreticalbasis of each phenomenon of fluorescence, followed by examples which illustrate the use of the phenomenon in the study of biochemical problems. The book contains numerous figures. It is felt that such graphical presentations contribute to pleasurable reading and increased understand ing. Separate chapters are devoted to fluorescence polarization, lifetimes, quenching, energy transfer, solvent effects, and excited state reactions. To enhance the usefulness of this work as a textbook, problems are included which illustrate the concepts described in each chapter. Furthermore, a separate chapter is devoted to the instrumentation used in fluorescence spectroscopy. This chapter will be especially valuable for those perform ing or contemplating fluorescence measurements. Such measurements are easily compromised by failure to consider a number of simple principles.
Author : Joseph R. Lakowicz
Publisher : Springer Science & Business Media
Page : 555 pages
File Size : 52,8 Mb
Release : 2006-04-18
Category : Science
ISBN : 9780306470707
Fluorescence spectroscopy continues its advance to more sophisticated methods and applications. As one looks over the previous decades, its appears that the first practical instruments for time-resolved measurements appeared in the 1970’s. The instrumentation and analysis methods for time-resolved fluorescence advanced rapidly throughout the 1980’s. Since 1990 we have witnessed a rapid migration of the principles of time-resolved fluorescence to cell biology and clinical appli- tions. Most recently, we have seen the introduction of multi-photon excitation, pump-probe and stimulated emission methods for studies of biological mac- molecules and for cellular imaging. These advanced topics are the subject of the present volume. Two-photon excitation was first predicted by Maria Goppert-Mayer in 1931, but was not experimentally observed until 1961. Observation of two-photon excitation required the introduction of lasers which provided adequate photon density for multi-photon absorption. Since the early observations of two-photon excitation in the 1960s, multi-photon spectroscopy has been limited to somewhat exotic applications of chemical physics, where it is used to study the electronic symmetry of small molecules. Placing one’s self back in 1980, it would be hard to imagine the use of multi-photon excitation in biophysics or cellular imaging.
Author : Anonim
Publisher : Unknown
Page : 212 pages
File Size : 48,5 Mb
Release : 1991
Category : Fluorescence microscopy
ISBN : UOM:39015032604160
Author : Markus Sauer,Johan Hofkens,Jörg Enderlein
Publisher : John Wiley & Sons
Page : 425 pages
File Size : 52,6 Mb
Release : 2010-12-23
Category : Science
ISBN : 9783527633524
Providing much-needed information on fluorescence spectroscopy and microscopy, this ready reference covers detection techniques, data registration, and the use of spectroscopic tools, as well as new techniques for improving the resolution of optical microscopy below the resolution gap. Starting with the basic principles, the book goes on to treat fluorophores and labeling, single-molecule fluorescence spectroscopy and enzymatics, as well as excited state energy transfer, and super-resolution fluorescence imaging. Examples show how each technique can help in obtaining detailed and refined information from individual molecular systems.
Author : Jens Götze
Publisher : Unknown
Page : 180 pages
File Size : 50,6 Mb
Release : 2000
Category : Cathodoluminescence
ISBN : STANFORD:36105029057259
Author : A.S. Marfunin
Publisher : Springer Science & Business Media
Page : 364 pages
File Size : 54,9 Mb
Release : 2012-12-06
Category : Science
ISBN : 9783642671128
The development of mineralogy, the evolutionary changes in compre hending the mineral substance of the earth are closely associated with the progress of research methods. Over a space of more than two and half centuries, from the goniometry of the mineral crystals to microscopic petrography and optical mineralogy, to crystal structure determinations, electron micros copy and electron diffraction and finally investigations into their electri cal, magnetic and mechanical properties, all this has led to the formation of the existing system of mineralogy, its notions, theories and to a proper description of minerals. However, no matter how great the variety of methods employed in mineralogy, they all come to a few aspects of substance characteristics. These are methods of determining the composition, structure and proper ties of the minerals. Thus the X-ray micro analyzer, the atom-absorption, neutron-activation, chromatographic and other analyses open up new opportunities for determining nothing else but the elementary com position of minerals.
Author : Bernard Valeur,Jean-Claude Brochon
Publisher : Springer Science & Business Media
Page : 496 pages
File Size : 41,7 Mb
Release : 2012-12-06
Category : Science
ISBN : 9783642568534
This first volume in the new Springer Series on Fluorescence brings together fundamental and applied research from this highly interdisciplinary and field, ranging from chemistry and physics to biology and medicine. Special attention is given to supramolecular systems, sensor applications, confocal microscopy and protein-protein interactions. This carefully edited collection of articles is an invaluable tool for practitioners and novices.
Author : Radek Šachl,Mariana Amaro
Publisher : Springer Nature
Page : 532 pages
File Size : 52,8 Mb
Release : 2023-04-27
Category : Science
ISBN : 9783031303623
This book provides the reader with an updated comprehensive view of the rapidly developing and fascinating field of fluorescence spectroscopy and microscopy. In recent years, fluorescence spectroscopy and microscopy have experienced rapid technological development, which has enabled the detection and monitoring of single molecules with high spatial and temporal resolution. Thanks to these developments, fluorescence has become an even more popular method in physical, biological and related fields. This book guides the reader through both basic and advanced fluorescence spectroscopy and microscopy approaches with a focus on their applications in membrane and protein biophysics. Each of the four parts: A - Fluorescence Spectroscopy, B - Fluorescence Microscopy, C - Applications of Fluorescence Spectroscopy and Microscopy to biological membranes and D - Applications of Fluorescence Spectroscopy to protein studies are written by experts within the field. The book is intended for both complete beginners who want to quickly orient themselves in the large number of existing fluorescent methods, as well as for advanced readers who are interested in particular methods and their proper use.