Novel Developments In Cryo Em Of Biological Molecules

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Novel Developments in Cryo‐EM of Biological Molecules

Joachim Frank
Novel Developments in Cryo EM of Biological Molecules Book PDF

Author : Joachim Frank
Publisher : CRC Press
Page : 553 pages
File Size : 48,7 Mb
Release : 2023-10-06
Category : Science
ISBN : 9781000989441

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Novel Developments in Cryo‐EM of Biological Molecules by Joachim Frank Pdf

Cryo-EM, as it is currently practiced in many laboratories, is limited to the visualization of molecules that are in thermal equilibrium at the time before freezing. A further limitation is that the existing software does not fully exploit the information that is contained in the images of large ensembles of molecules in thermal equilibrium. This book is a collection of recent articles by the author, reprinted with introductions, and they mainly describe two novel methods in cryo-EM, one computational and the other experimental that requires the use of a microfluidic device. Both methods have the capacity to shed light on the dynamic behavior of biomolecules. Combined, they greatly expand the range of applications of cryo-EM. The book describes a successful approach in which, based on cryo-EM data, all states visited by the molecule in thermal equilibrium are mapped by manifold embedding—a method of geometric machine learning—and the energy landscape of the molecule is derived. It also discusses methods and biological results of time-resolved cryo-EM, following a reaction in a non-equilibrium system over a short period of time and resulting in the capture of short-lived states that have been inaccessible by standard methods of cryo-EM.

Single-particle Cryo-EM of Biological Macromolecules

Robert M. Glaeser,Eva Nogales,Wah Chiu
Single particle Cryo EM of Biological Macromolecules Book PDF

Author : Robert M. Glaeser,Eva Nogales,Wah Chiu
Publisher : Unknown
Page : 0 pages
File Size : 43,8 Mb
Release : 2021
Category : Biomolecules
ISBN : 0750330392

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Single-particle Cryo-EM of Biological Macromolecules by Robert M. Glaeser,Eva Nogales,Wah Chiu Pdf

This edited book is written for students, postdocs and established investigators who want to enter the field of single-particle cryo-EM. This is a recently developed method to determine high-resolution structures of biological macromolecules. A major strength is the fact that cryo-EM does not require prior crystallization of protein complexes. It is especially well suited for larger complexes and molecular machines. This book provides a comprehensive, accessible and authoritative introduction to the field. It covers all necessary background, ranging from the underlying concepts to practical aspects such as specimen preparation, data collection, data analysis, and the final validation of results.

Single-particle Cryo-electron Microscopy

Joachim Frank
Single particle Cryo electron Microscopy Book PDF

Author : Joachim Frank
Publisher : World Scientific Publishing Company
Page : 0 pages
File Size : 45,5 Mb
Release : 2017-12-31
Category : Electron microscopy
ISBN : 9813234857

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Single-particle Cryo-electron Microscopy by Joachim Frank Pdf

The book reproduces 55 of more than 300 articles written by the author, representing milestones in methods development of single-particle cryo-EM as well as important results obtained by this technique in the study of biological macromolecules and their interactions. Importantly, neither symmetries nor ordered arrangements (as in two-dimensional crystals, helical assemblies, icosahedral viruses) are required. Although the biological applications are mainly in the area of ribosome structure and function, the elucidation of membrane channel structures and their activation and gating mechanisms are represented, as well. The book is introduced by a commentary that explains the original development of concepts, describes the contributions of the author's colleagues and students, and shows how challenges were overcome as the technique matured. Along the way, the ribosome served as an example for a macromolecule with intricate structure and conformational dynamics that pose challenges for three-dimensional visualization. Toward the end of the book -- bringing us to the present time -- molecular structures with near-atomic resolution are presented, and a novel type of computational analysis, manifold embedding, is introduced. Single-particle cryo-EM is currently revolutionizing structural biology, presenting a powerful alternative to X-ray crystallography as a means to solve the structure of biological macromolecules. The book presents in one place a number of articles containing key advances in mathematical and computational methods leading up to the present time. Secondly, the development of the technique over the years is reflected by ever-expanding discoveries in the field of ribosome structure and function. Thirdly, as all histories of ideas, the history of concepts pertaining to this new method of visualization is fascinating all in itself.

Cryo-Electron Tomography

Friedrich Förster,Ariane Briegel
Cryo Electron Tomography Book PDF

Author : Friedrich Förster,Ariane Briegel
Publisher : Springer
Page : 0 pages
File Size : 48,8 Mb
Release : 2024-05-09
Category : Science
ISBN : 3031511700

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Cryo-Electron Tomography by Friedrich Förster,Ariane Briegel Pdf

This book presents key aspects and recent developments of cryogenic sample electron tomography (cryo-ET) methodology, authored by leading experts in the field. Understanding structure and function of biomolecules in the context of cells is a new frontier in cellular and structural biology. To facilitate such research, cryo-ET is a key method to visualize the molecules of life in their native settings. Cryo-ET enables the imaging of samples that are preserved in a near-native state, at (macro)-molecular resolution and in three dimensions. Thus, this technique is a unique tool to gain insights into how biomolecules collaborate in orchestrating fundamental biological processes, how mutations cause diseases, pathogens cause infections, and to develop novel therapeutics to treat such illnesses. This book provides a unique reference for the emerging field of cryo-ET. The topics covered range from the fundamental principles of imaging to sample preparation, data analysis,and data sharing within the scientific community. It serves as a valuable resource for the next generation of structural biologists, making it suitable both for undergraduate students studying biochemistry, biophysics, and molecular biology and highly valuable for the more experienced and specialized PhD student. Furthermore, it stands as a state-of-the–art source of knowledge for the established senior scientist within the field of structural biology.

cryoEM

Tamir Gonen,Brent L. Nannenga
cryoEM Book PDF

Author : Tamir Gonen,Brent L. Nannenga
Publisher : Humana
Page : 350 pages
File Size : 44,7 Mb
Release : 2020-12-27
Category : Science
ISBN : 1071609653

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cryoEM by Tamir Gonen,Brent L. Nannenga Pdf

This volume details the most up-to-date cryo-EM techniques from leading researchers. Chapters are organized into four parts with emphasis on electron cryotomography, single particle analysis, and the crystal based cryo-EM methods of 2D electron crystallography, and MicroED for the study of 3D crystals. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, CryoEM: Methods and Protocols aims to serve as an excellent resource on cryo-EM and can serve as the foundation for new researchers to this growing field in structural biology.

The Resolution Revolution: Recent Advances In cryoEM

Anonim
The Resolution Revolution Recent Advances In cryoEM Book PDF

Author : Anonim
Publisher : Academic Press
Page : 486 pages
File Size : 41,5 Mb
Release : 2016-08-26
Category : Medical
ISBN : 9780128054352

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The Resolution Revolution: Recent Advances In cryoEM by Anonim Pdf

cryoEM, a new volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods and new developments in recording images, the creation, evaluation and validation of 3D maps from the images, model building into maps and refinement of the resulting atomic structures, and applications of essentially single particle methods to helical structures and to sub-tomogram averaging. Continues the legacy of this premier serial with quality chapters authored by leaders in the field Covers research methods that determine the structures of biological molecules, a vital step for understanding their function Contains the technical developments underpinning the advances of cryoEM and captures the exciting insights that have resulted

Biological Macromolecules

Amit Kumar Nayak,Amal Kumar Dhara,Dilipkumar Pal
Biological Macromolecules Book PDF

Author : Amit Kumar Nayak,Amal Kumar Dhara,Dilipkumar Pal
Publisher : Academic Press
Page : 673 pages
File Size : 46,6 Mb
Release : 2021-11-23
Category : Science
ISBN : 9780323856386

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Biological Macromolecules by Amit Kumar Nayak,Amal Kumar Dhara,Dilipkumar Pal Pdf

Biological Macromolecules: Bioactivity and Biomedical Applications presents a comprehensive study of biomacromolecules and their potential use in various biomedical applications. Consisting of four sections, the book begins with an overview of the key sources, properties and functions of biomacromolecules, covering the foundational knowledge required for study on the topic. It then progresses to a discussion of the various bioactive components of biomacromolecules. Individual chapters explore a range of potential bioactivities, considering the use of biomacromolecules as nutraceuticals, antioxidants, antimicrobials, anticancer agents, and antidiabetics, among others. The third section of the book focuses on specific applications of biomacromolecules, ranging from drug delivery and wound management to tissue engineering and enzyme immobilization. This focus on the various practical uses of biological macromolecules provide an interdisciplinary assessment of their function in practice. The final section explores the key challenges and future perspectives on biological macromolecules in biomedicine. Covers a variety of different biomacromolecules, including carbohydrates, lipids, proteins, and nucleic acids in plants, fungi, animals, and microbiological resources Discusses a range of applicable areas where biomacromolecules play a significant role, such as drug delivery, wound management, and regenerative medicine Includes a detailed overview of biomacromolecule bioactivity and properties Features chapters on research challenges, evolving applications, and future perspectives

Macromolecular Crystallography

Maria Armenia Carrondo,Paola Spadon
Macromolecular Crystallography Book PDF

Author : Maria Armenia Carrondo,Paola Spadon
Publisher : Springer
Page : 208 pages
File Size : 50,8 Mb
Release : 2011-12-01
Category : Science
ISBN : 9789400725300

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Macromolecular Crystallography by Maria Armenia Carrondo,Paola Spadon Pdf

This volume is a collection of the contributions presented at the 42nd Erice Crystallographic Course whose main objective was to train the younger generation on advanced methods and techniques for examining structural and dynamic aspects of biological macromolecules. The papers review the techniques used to study protein assemblies and their dynamics, including X-ray diffraction and scattering, electron cryo-electron microscopy, electro nanospray mass spectrometry, NMR, protein docking and molecular dynamics. A key theme throughout the book is the dependence of modern structural science on multiple experimental and computational techniques, and it is the development of these techniques and their integration that will take us forward in the future.

Scanning Electron Microscopy for the Life Sciences

Heide Schatten
Scanning Electron Microscopy for the Life Sciences Book PDF

Author : Heide Schatten
Publisher : Cambridge University Press
Page : 275 pages
File Size : 41,9 Mb
Release : 2013
Category : Science
ISBN : 9780521195997

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Scanning Electron Microscopy for the Life Sciences by Heide Schatten Pdf

A guide to modern scanning electron microscopy instrumentation, methodology and techniques, highlighting novel applications to cell and molecular biology.

Electron Crystallography of Biological Macromolecules

Robert M. Glaeser
Electron Crystallography of Biological Macromolecules Book PDF

Author : Robert M. Glaeser
Publisher : Oxford University Press, USA
Page : 500 pages
File Size : 48,5 Mb
Release : 2007
Category : Science
ISBN : UOM:39015069321720

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Electron Crystallography of Biological Macromolecules by Robert M. Glaeser Pdf

This book provides a complete introduction to all major topics needed in order to use electron microscopy as a research tool in structural biology. Written by a group of 5 well-known pioneers of the field of electron cryo-microscopy of biological macromolecules, this book offers a depth of knowledge and expertise that could only be replicated from the primary literature with great difficulty.

Fundamentals of Molecular Structural Biology

Subrata Pal
Fundamentals of Molecular Structural Biology Book PDF

Author : Subrata Pal
Publisher : Academic Press
Page : 518 pages
File Size : 42,9 Mb
Release : 2019-08-13
Category : Science
ISBN : 9780128148563

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Fundamentals of Molecular Structural Biology by Subrata Pal Pdf

Fundamentals of Molecular Structural Biology reviews the mathematical and physical foundations of molecular structural biology. Based on these fundamental concepts, it then describes molecular structure and explains basic genetic mechanisms. Given the increasingly interdisciplinary nature of research, early career researchers and those shifting into an adjacent field often require a "fundamentals" book to get them up-to-speed on the foundations of a particular field. This book fills that niche. Provides a current and easily digestible resource on molecular structural biology, discussing both foundations and the latest advances Addresses critical issues surrounding macromolecular structures, such as structure-based drug discovery, single-particle analysis, computational molecular biology/molecular dynamic simulation, cell signaling and immune response, macromolecular assemblies, and systems biology Presents discussions that ultimately lead the reader toward a more detailed understanding of the basis and origin of disease

Biological Field Emission Scanning Electron Microscopy, 2 Volume Set

Roland A. Fleck,Bruno M. Humbel
Biological Field Emission Scanning Electron Microscopy 2 Volume Set Book PDF

Author : Roland A. Fleck,Bruno M. Humbel
Publisher : John Wiley & Sons
Page : 741 pages
File Size : 55,9 Mb
Release : 2019-04-29
Category : Science
ISBN : 9781118654064

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Biological Field Emission Scanning Electron Microscopy, 2 Volume Set by Roland A. Fleck,Bruno M. Humbel Pdf

The go‐to resource for microscopists on biological applications of field emission gun scanning electron microscopy (FEGSEM) The evolution of scanning electron microscopy technologies and capability over the past few years has revolutionized the biological imaging capabilities of the microscope—giving it the capability to examine surface structures of cellular membranes to reveal the organization of individual proteins across a membrane bilayer and the arrangement of cell cytoskeleton at a nm scale. Most notable are their improvements for field emission scanning electron microscopy (FEGSEM), which when combined with cryo-preparation techniques, has provided insight into a wide range of biological questions including the functionality of bacteria and viruses. This full-colour, must-have book for microscopists traces the development of the biological field emission scanning electron microscopy (FEGSEM) and highlights its current value in biological research as well as its future worth. Biological Field Emission Scanning Electron Microscopy highlights the present capability of the technique and informs the wider biological science community of its application in basic biological research. Starting with the theory and history of FEGSEM, the book offers chapters covering: operation (strengths and weakness, sample selection, handling, limitations, and preparation); Commercial developments and principals from the major FEGSEM manufacturers (Thermo Scientific, JEOL, HITACHI, ZEISS, Tescan); technical developments essential to bioFEGSEM; cryobio FEGSEM; cryo-FIB; FEGSEM digital-tomography; array tomography; public health research; mammalian cells and tissues; digital challenges (image collection, storage, and automated data analysis); and more. Examines the creation of the biological field emission gun scanning electron microscopy (FEGSEM) and discusses its benefits to the biological research community and future value Provides insight into the design and development philosophy behind current instrument manufacturers Covers sample handling, applications, and key supporting techniques Focuses on the biological applications of field emission gun scanning electron microscopy (FEGSEM), covering both plant and animal research Presented in full colour An important part of the Wiley-Royal Microscopical Series, Biological Field Emission Scanning Electron Microscopy is an ideal general resource for experienced academic and industrial users of electron microscopy—specifically, those with a need to understand the application, limitations, and strengths of FEGSEM.

Biomolecular and Bioanalytical Techniques

Vasudevan Ramesh
Biomolecular and Bioanalytical Techniques Book PDF

Author : Vasudevan Ramesh
Publisher : John Wiley & Sons
Page : 576 pages
File Size : 48,9 Mb
Release : 2019-06-10
Category : Science
ISBN : 9781119483960

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Biomolecular and Bioanalytical Techniques by Vasudevan Ramesh Pdf

An essential guide to biomolecular and bioanalytical techniques and their applications Biomolecular and Bioanalytical Techniques offers an introduction to, and a basic understanding of, a wide range of biophysical techniques. The text takes an interdisciplinary approach with contributions from a panel of distinguished experts. With a focus on research, the text comprehensively covers a broad selection of topics drawn from contemporary research in the fields of chemistry and biology. Each of the internationally reputed authors has contributed a single chapter on a specific technique. The chapters cover the specific technique’s background, theory, principles, technique, methodology, protocol and applications. The text explores the use of a variety of analytical tools to characterise biological samples. The contributors explain how to identify and quantify biochemically important molecules, including small molecules as well as biological macromolecules such as enzymes, antibodies, proteins, peptides and nucleic acids. This book is filled with essential knowledge and explores the skills needed to carry out the research and development roles in academic and industrial laboratories. A technique-focused book that bridges the gap between an introductory text and a book on advanced research methods Provides the necessary background and skills needed to advance the research methods Features a structured approach within each chapter Demonstrates an interdisciplinary approach that serves to develop independent thinking Written for students in chemistry, biological, medical, pharmaceutical, forensic and biophysical sciences, Biomolecular and Bioanalytical Techniques is an in-depth review of the most current biomolecular and bioanalytical techniques in the field.

Recent Advances in Electron Cryomicroscopy

Steven J. Ludtke,B. V. Venkataram Prasad
Recent Advances in Electron Cryomicroscopy Book PDF

Author : Steven J. Ludtke,B. V. Venkataram Prasad
Publisher : Academic Press
Page : 154 pages
File Size : 42,5 Mb
Release : 2011
Category : Biochemistry
ISBN : 9780123865076

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Recent Advances in Electron Cryomicroscopy by Steven J. Ludtke,B. V. Venkataram Prasad Pdf

Electron cryomicroscopy, is a form of transmission electron microscopy (EM) where the sample is studied at cryogenic temperatures (generally liquid nitrogen temperatures). Cryo-EM is developing popularity in structural biology. This volume from the Advances in Protein Chemistry and Structural Biology series is Part B and covers essential topics.

Handbook of Cryo-Preparation Methods for Electron Microscopy

Annie Cavalier,Daniele Spehner,Bruno M. Humbel
Handbook of Cryo Preparation Methods for Electron Microscopy Book PDF

Author : Annie Cavalier,Daniele Spehner,Bruno M. Humbel
Publisher : CRC Press
Page : 0 pages
File Size : 50,8 Mb
Release : 2008-10-02
Category : Science
ISBN : 0849372275

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Handbook of Cryo-Preparation Methods for Electron Microscopy by Annie Cavalier,Daniele Spehner,Bruno M. Humbel Pdf

While new discoveries have led to much dramatic growth in cryo-electron microscopy, researchers will never be able to take full advantage if they lack access to the details that make these techniques understandable and applicable. The Handbook of Cryo-Preparation Methods for Electron Microscopy provides researchers with a complete reference that will show them how to equip their labs with the right materials and methods to take full advantage of the latest advances. This pioneering work brings together a group of internationally renowned researchers, some the very inventors of the methods they describe, to share their knowledge and recipes. Taking care to explain the history behind the techniques and to demonstrate their use, this book presents the latest theory, principles, and protocols supplemented by hundreds of illustrations. Contributions to this handbook – Ö Describe cryo-methods aimed at perfect preservation for fine structural analysis Ö Teach how to arrest physiological processes by cryo-fixation Ö Reveal the secrets for high-resolution snapshots of life by CEMOVIS Ö Cover applications of cryo-methods such as electron crystallography, 2D/3D structure analysis, protein localization, and cryo-electron tomography Ö Demonstrate the use of vitreous water as an intermediate step for localization of biological ions and molecules Ö Present hybrid methods of freeze-substitution and freeze-drying for immunolabeling and determining molecular geography Ö Illustrate freeze-fracture, cryo-ultramicrotomy, and resin embedding Ö Highlight the Tokuyasu method and the new rehydration technique for highly efficient immunolabeling Ö Include summary tables to compare and obtain appropriate criteria Includes a wealth of immediate reference material for daily use With clear, step-by-step recipes and lists of tools, ingredients, and suppliers, this handbookprovides researchers with the knowledge and techniques to adopt the latest cryo-methods to their current research.