Patient Derived Tumor Xenograft Models

Author : Rajesh Uthamanthil,Peggy Tinkey,Elisa de Stanchina
Publisher : Academic Press
Page : 486 pages
File Size : 40,6 Mb
Release : 2016-10-13
Category : Medical
ISBN : 9780128040614

Get Book

Patient Derived Tumor Xenograft Models by Rajesh Uthamanthil,Peggy Tinkey,Elisa de Stanchina Pdf

Patient Derived Tumor Xenograft Models: Promise, Potential and Practice offers guidance on how to conduct PDX modeling and trials, including how to know when these models are appropriate for use, and how the data should be interpreted through the selection of immunodeficient strains. In addition, proper methodologies suitable for growing different type of tumors, acquisition of pathology, genomic and other data about the tumor, potential pitfalls, and confounding background pathologies that occur in these models are also included, as is a discussion of the facilities and infrastructure required to operate a PDX laboratory. Offers guidance on data interpretation and regulatory aspects Provides useful techniques and strategies for working with PDX models Includes practical tools and potential pitfalls for best practices Compiles all knowledge of PDX models research in one resource Presents the results of first ever global survey on standards of PDX development and usage in academia and industry

Author : Robert M. Hoffman
Publisher : Humana
Page : 0 pages
File Size : 54,6 Mb
Release : 2018-06-15
Category : Medical
ISBN : 3319861581

Get Book

Patient-Derived Mouse Models of Cancer by Robert M. Hoffman Pdf

This text highlights seminal discoveries and also provides comprehensive and state-of the-art approach to mouse models of human patient tumors. These areas include training, basic techniques, as well as general troubleshooting. Subsequent chapters focus on the different mouse models of patient tumors including the various strains of immunodeficient mice currently available and the transplantation techniques that can be used as well as state-of-the-art imaging techniques. Practical applications of the models from drug discovery, genome analysis to personalized treatment are also covered. Written by experts in that field, each of these sections address these critical issues. A brief review of the existing literature addressing the particular topic follows in each section. Presently, there is no single source to provide information on technique and uses of mouse models of human patient tumors. Patient-Derived Mouse Models of Cancer will satisfy this need for cancer researchers, oncologists, pharmaceutical and biotechnology industry scientists as well as molecular biologists studying in vivo systems

Author : Yuzhuo Wang,Dong Lin,Peter W. Gout
Publisher : Springer
Page : 204 pages
File Size : 52,5 Mb
Release : 2017-06-27
Category : Medical
ISBN : 9783319558257

Get Book

Patient-Derived Xenograft Models of Human Cancer by Yuzhuo Wang,Dong Lin,Peter W. Gout Pdf

This book provides a comprehensive, state-of-the-art review of PDX cancer models. In separately produced chapters, the history and evolution of PDX models is reviewed, methods of PDX model development are compared in detail, characteristics of available established models are presented, current applications are summarized and new perspectives about use of PDX models are proposed. Each chapter is written by a world-renowned expert who is conducting cutting-edge research in the field. Each of the subsections provide a comprehensive review of existing literature addressing the particular topic followed by a conclusive paragraph detailing future directions. Extensive illustrations make this an interactive text. Patient-Derived Xenograft Models of Human Cancer will serve as a highly useful resource for researchers and clinicians dealing with, or interested in, this important topic. It will provide a concise yet comprehensive summary of the current status of the field that will help guide preclinical and clinical applications as well as stimulate investigative efforts. This book will propagate innovative concepts and prompt the development of ground-breaking technological solutions in this field.

Author : Shay Soker,Aleksander Skardal
Publisher : Humana Press
Page : 213 pages
File Size : 42,7 Mb
Release : 2017-10-20
Category : Medical
ISBN : 9783319605111

Get Book

Tumor Organoids by Shay Soker,Aleksander Skardal Pdf

Cancer cell biology research in general, and anti-cancer drug development specifically, still relies on standard cell culture techniques that place the cells in an unnatural environment. As a consequence, growing tumor cells in plastic dishes places a selective pressure that substantially alters their original molecular and phenotypic properties.The emerging field of regenerative medicine has developed bioengineered tissue platforms that can better mimic the structure and cellular heterogeneity of in vivo tissue, and are suitable for tumor bioengineering research. Microengineering technologies have resulted in advanced methods for creating and culturing 3-D human tissue. By encapsulating the respective cell type or combining several cell types to form tissues, these model organs can be viable for longer periods of time and are cultured to develop functional properties similar to native tissues. This approach recapitulates the dynamic role of cell–cell, cell–ECM, and mechanical interactions inside the tumor. Further incorporation of cells representative of the tumor stroma, such as endothelial cells (EC) and tumor fibroblasts, can mimic the in vivo tumor microenvironment. Collectively, bioengineered tumors create an important resource for the in vitro study of tumor growth in 3D including tumor biomechanics and the effects of anti-cancer drugs on 3D tumor tissue. These technologies have the potential to overcome current limitations to genetic and histological tumor classification and development of personalized therapies.

Author : Beverly A. Teicher
Publisher : Springer Science & Business Media
Page : 994 pages
File Size : 54,5 Mb
Release : 2001-11-07
Category : Medical
ISBN : 9781592591008

Get Book

Tumor Models in Cancer Research by Beverly A. Teicher Pdf

Beverly A. Teicher and a panel of leading experts comprehensively describe for the first time in many years the state-of-the-art in animal tumor model research. The wide array of models detailed form the basis for the selection of compounds and treatments that go into clinical testing of patients, and include syngeneic models, human tumor xenograft models, orthotopic models, metastatic models, transgenic models, and gene knockout models. Synthesizing many years experience with all the major in vivo models currently available for the study of malignant disease, Tumor Models in Cancer Research provides preclinical and clinical cancer researchers alike with a comprehensive guide to the selection of these models, their effective use, and the optimal interpretation of their results.

Author : Dong-Young Noh,Wonshik Han,Masakazu Toi
Publisher : Springer Nature
Page : 630 pages
File Size : 43,7 Mb
Release : 2021-05-13
Category : Medical
ISBN : 9789813296206

Get Book

Translational Research in Breast Cancer by Dong-Young Noh,Wonshik Han,Masakazu Toi Pdf

This book describes recent advances in translational research in breast cancer and presents emerging applications of this research that promise to have meaningful impacts on diagnosis and treatment. It introduces ideas and materials derived from the clinic that have been brought to "the bench" for basic research, as well as findings that have been applied back to "the bedside". Detailed attention is devoted to breast cancer biology and cell signaling pathways and to cancer stem cell and tumor heterogeneity in breast cancer. Various patient-derived research models are discussed, and a further focus is the role of biomarkers in precision medicine for breast cancer patients. Next-generation clinical research receives detailed attention, addressing the increasingly important role of big data in breast cancer research and a wide range of other emerging developments. An entire section is also devoted to the management of women with high-risk breast cancer. Translational Research in Breast Cancer will help clinicians and scientists to optimize their collaboration in order to achieve the common goal of conquering breast cancer.

Author : Beverly A. Teicher,Paul A. Andrews
Publisher : Springer Science & Business Media
Page : 684 pages
File Size : 47,6 Mb
Release : 2004-02-01
Category : Medical
ISBN : 9781592597390

Get Book

Anticancer Drug Development Guide by Beverly A. Teicher,Paul A. Andrews Pdf

This unique volume traces the critically important pathway by which a "molecule" becomes an "anticancer agent. " The recognition following World War I that the administration of toxic chemicals such as nitrogen mustards in a controlled manner could shrink malignant tumor masses for relatively substantial periods of time gave great impetus to the search for molecules that would be lethal to specific cancer cells. Weare still actively engaged in that search today. The question is how to discover these "anticancer" molecules. Anticancer Drug Development Guide: Preclinical Screening, Clinical Trials, and Approval, Second Edition describes the evolution to the present of preclinical screening methods. The National Cancer Institute's high-throughput, in vitro disease-specific screen with 60 or more human tumor cell lines is used to search for molecules with novel mechanisms of action or activity against specific phenotypes. The Human Tumor Colony-Forming Assay (HTCA) uses fresh tumor biopsies as sources of cells that more nearly resemble the human disease. There is no doubt that the greatest successes of traditional chemotherapy have been in the leukemias and lymphomas. Since the earliest widely used in vivo drug screening models were the murine L 1210 and P388 leukemias, the community came to assume that these murine tumor models were appropriate to the discovery of "antileukemia" agents, but that other tumor models would be needed to discover drugs active against solid tumors.

Author : Mohamed I. Saad
Publisher : Springer Nature
Page : 250 pages
File Size : 40,5 Mb
Release : 2024-05-19
Category : Electronic
ISBN : 9781071638583

Get Book

Patient-Derived Xenografts by Mohamed I. Saad Pdf

Author : Robert M. Hoffman
Publisher : Springer
Page : 296 pages
File Size : 40,5 Mb
Release : 2017-08-01
Category : Medical
ISBN : 9783319574240

Get Book

Patient-Derived Mouse Models of Cancer by Robert M. Hoffman Pdf

This text highlights seminal discoveries and also provides comprehensive and state-of the-art approach to mouse models of human patient tumors. These areas include training, basic techniques, as well as general troubleshooting. Subsequent chapters focus on the different mouse models of patient tumors including the various strains of immunodeficient mice currently available and the transplantation techniques that can be used as well as state-of-the-art imaging techniques. Practical applications of the models from drug discovery, genome analysis to personalized treatment are also covered. Written by experts in that field, each of these sections address these critical issues. A brief review of the existing literature addressing the particular topic follows in each section. Presently, there is no single source to provide information on technique and uses of mouse models of human patient tumors. Patient-Derived Mouse Models of Cancer will satisfy this need for cancer researchers, oncologists, pharmaceutical and biotechnology industry scientists as well as molecular biologists studying in vivo systems

Author : Michael Breitenbach,Jens Hoffmann
Publisher : Frontiers Media SA
Page : 176 pages
File Size : 45,5 Mb
Release : 2019-02-05
Category : Electronic
ISBN : 9782889457014

Get Book

Cancer Models by Michael Breitenbach,Jens Hoffmann Pdf

Cancer research, like research on other diseases, highly depends on representative and reliable model systems. In the Research Topic “Cancer Models”, we collected original papers and review articles addressing the topic of tumor modeling from molecular biology, biochemistry, microorganisms, cells and organoids, fishes, animals and xenografts, up to computational cancer models and patient data analysis. This representative eBook describes that there is not a single molecular defined tumor but rather a heterogenic and highly variable complex of different individual diseases. This is what makes research on cancer so difficult, expensive, and explains the broad number of models needed for research. Our authors describe new next-generation sequencing-based methods to analyze complex patterns of chromosomal aberrations in order to understand the molecular biology of tumorigenesis as well as the role of cellular senescence and dormancy in the aetiology of tumor formation and development of therapy resistance of tumors. The current developments on 3D cultures are thoroughly reviewed, as these models help to overcome the current limitations of cell cultures and allow a more accurate mimicry of the native cancer tissue, including cellular heterogeneity and restore specific biochemical and morphological. Reviews about tumor models in zebrafish, different transgenic mouse strains and pigs conclude the book. In the final two chapters of this volume, the authors discuss the theoretical and mathematical models developed in cancer research.

Author : Hatim E. Sabaawy,Massimo Broggini,Shiv K. Gupta
Publisher : Frontiers Media SA
Page : 183 pages
File Size : 51,5 Mb
Release : 2023-07-31
Category : Medical
ISBN : 9782832530542

Get Book

Patient-derived Tumor Models for Drug Development by Hatim E. Sabaawy,Massimo Broggini,Shiv K. Gupta Pdf

Author : Boris Rubinsky
Publisher : Springer Science & Business Media
Page : 320 pages
File Size : 54,5 Mb
Release : 2009-11-25
Category : Technology & Engineering
ISBN : 9783642054204

Get Book

Irreversible Electroporation by Boris Rubinsky Pdf

Non-thermal irreversible electroporation is a new minimally invasive surgical p- cedure with unique molecular selectivity attributes – in fact it may be considered the first clinical molecular surgery procedure. Non-thermal irreversible electro- ration is a molecular selective mode of cell ablation that employs brief electrical fields to produce nanoscale defects in the cell membrane, which can lead to cell death, without an effect on any of the other tissue molecules. The electrical fields can be produced through contact by insertion of electrode needles around the undesirable tissue and non-invasively by electromagnetic induction. This new - dition to the medical armamentarium requires the active involvement and is of interest to clinical physicians, medical researchers, mechanical engineers, che- cal engineers, electrical engineers, instrumentation designers, medical companies and many other fields and disciplines that were never exposed in their training to irreversible electroporation or to a similar concept. This edited book is designed to be a comprehensive introduction to the field of irreversible electroporation to those that were not exposed or trained in the field before and can also serve as a reference manual. Irreversible electroporation is broad and interdisciplinary. Therefore, we have made an attempt to cover every one of the various aspects of the field from an introductory basic level to state of the art.

Author : Anonim
Publisher : BoD – Books on Demand
Page : 320 pages
File Size : 52,9 Mb
Release : 2020-04-08
Category : Medical
ISBN : 9781789853490

Get Book

Tumor Progression and Metastasis by Anonim Pdf

This book offers significant coverage on different aspects of cancer from risk factors to the mechanisms leading to tumor progression and metastasis. Although tremendous progress has been made in cancer research and treatment, cancer metastasis remains a major unmet clinical need. The life and death of many cancer patients hangs on the degree of metastasis. This book provides new perspectives for diagnosis and cancer therapy. It includes new technologies and a new basis for current cancer therapies. To guarantee the high quality of this book, important topics are included and rigorously discussed in a simple and authentic way. The book addresses important challenges governing tumor progression and metastasis and brings new responses to both diagnosis and therapy. This book is a great source of knowledge and will be useful for researchers, medical doctors, oncologists, graduate and medical students, continued medical educators, health care providers, and all individuals interested in understanding cancer and its challenges.

Author : Ulrike S. Stein
Publisher : Humana
Page : 348 pages
File Size : 54,9 Mb
Release : 2022-04-03
Category : Medical
ISBN : 1071613529

Get Book

Metastasis by Ulrike S. Stein Pdf

This volume provides the most recent developments and methodologies on metastatic process, formation, and detection. Chapters guide readers through functional metastasis in vitro assays, non-mouse and mice metastasis models, methods for imaging metastasis, analyzing the tumor microenvironment, senescence and inflammation with respect to metastasis, methods to investgate the premetastatic/ metastatic, detecting biomarkers in patient, and bioinformatics to simulate the metastatic process. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Metastasis: Methods and Protocols aims to be a useful practical guide to researches to help further their study in this field.

Author : Guido Bocci,Giulio Francia
Publisher : Springer
Page : 295 pages
File Size : 49,9 Mb
Release : 2014-09-04
Category : Medical
ISBN : 9783662436042

Get Book

Metronomic Chemotherapy by Guido Bocci,Giulio Francia Pdf

This book analyzes all aspects of metronomic chemotherapy, a new approach involving low-dose, long-term, and frequently administered therapy that has preclinical and clinical activity in various tumors. After an opening section on the pharmacological bases of metronomic chemotherapy, including its antiangiogenic effects and impact on immunity, preclinical studies on various classes of drug are discussed. Clinical applications of metronomic chemotherapy in a wide variety of tumors are then addressed in detail, with description of the results of all published studies. The clinical pharmacology of metronomic chemotherapy is also considered in depth, encompassing pharmacokinetics, pharmacogenetics, pharmacoeconomics, and adverse drug reactions. The book closes by describing the role of this therapy in the veterinarian clinic.