Spliceosomal Pre Mrna Splicing

Author : Klemens J. Hertel
Publisher : Humana
Page : 0 pages
File Size : 52,8 Mb
Release : 2016-09-03
Category : Medical
ISBN : 149396044X

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Spliceosomal Pre-mRNA Splicing by Klemens J. Hertel Pdf

Providing a guide to classical experimental approaches to decipher splicing mechanisms and experimental strategies that rely on novel multi-disciplinary approaches, Spliceosomal Pre-mRNA Splicing: Methods and Protocols describes the theory of alternative pre-mRNA splicing in seven introductory chapters and then introduces protocols and their theoretical background relevant for a variety of experimental research. These protocol chapters cover basic methods to detect splicing events, analyses of alternative pre-mRNA splicing in vitro and in vivo manipulation of splicing events and high-throughput and bioinformatic analyses of alternative splicing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and tips on troubleshooting and avoiding known pitfalls. Comprehensive and practical, Spliceosomal Pre-mRNA Splicing: Methods and Protocols will aid newcomers and seasoned molecular biologists in understanding the fascinating world of alternative splicing with the ultimate goal of paving the way for many new discoveries to come.

Author : Stefan Stamm,Chris Smith,Reinhard Lührmann
Publisher : John Wiley & Sons
Page : 660 pages
File Size : 52,5 Mb
Release : 2012-08-01
Category : Science
ISBN : 9783527647989

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Alternative pre-mRNA Splicing by Stefan Stamm,Chris Smith,Reinhard Lührmann Pdf

This book was written for graduate and medical students, as well as clinicians and postdoctoral researchers. It describes the theory of alternative pre-mRNA splicing in twelve introductory chapters and then introduces protocols and their theoretical background relevant for experimental research. These 43 practical chapters cover: Basic methods, Detection of splicing events, Analysis of alternative pre-mRNA splicing in vitro and in vivo, Manipulation of splicing events, and Bioinformatic analysis of alternative splicing. A theoretical introduction and practical guide for molecular biologists, geneticists,clinicians and every researcher interested in alternative splicing. Website: www.wiley-vch.de/home/splicing

Author : Stefan Stamm,Chris Smith,Reinhard Lührmann
Publisher : John Wiley & Sons
Page : 660 pages
File Size : 41,8 Mb
Release : 2012-02-13
Category : Science
ISBN : 9783527326068

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Alternative pre-mRNA Splicing by Stefan Stamm,Chris Smith,Reinhard Lührmann Pdf

Der definitive Leitfaden zum RNA-Splicing - ideal für alle Kliniker, die sich mit genetischen Erkrankungen befassen, insbesondere natürlich für RNA-Forscher. Website: www.wiley-vch.de/home/splicing

Author : Sabine Müller,Benoît Masquida,Wade Winkler
Publisher : John Wiley & Sons
Page : 81 pages
File Size : 43,6 Mb
Release : 2021-07-09
Category : Science
ISBN : 9783527814534

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Ribozymes by Sabine Müller,Benoît Masquida,Wade Winkler Pdf

Ribozymes Provides comprehensive coverage of a core field in the molecular biosciences, bringing together decades of knowledge from the world’s top professionals in the field Timely and unique in its breadth of content, this all-encompassing and authoritative reference on ribozymes documents the great diversity of nucleic acid-based catalysis. It integrates the knowledge gained over the past 35 years in the field and features contributions from virtually every leading expert on the subject. Ribozymes is organized into six major parts. It starts by describing general principles and strategies of nucleic acid catalysis. It then introduces naturally occurring ribozymes and includes the search for new catalytic motifs or novel genomic locations of known motifs. Next, it covers the development and design of engineered ribozymes, before moving on to DNAzymes as a close relative of ribozymes. The next part examines the use of ribozymes for medicinal and environmental diagnostics, as well as for therapeutic tools. It finishes with a look at the tools and methods in ribozyme research, including the techniques and assays for structural and functional characterization of nucleic acid catalysts. The first reference to tie together all aspects of the multi-faceted field of ribozymes Features more than 30 comprehensive chapters in two volumes Covers the chemical principles of RNA catalysis; naturally occurring ribozymes, engineered ribozymes; DNAzymes; ribozymes as tools in diagnostics and therapy, and tools and methods to study ribozymes Includes first-hand accounts of concepts, techniques, and applications by a team of top international experts from leading academic institutions Dedicates half of its content to methods and practical applications, ranging from bioanalytical tools to medical diagnostics to therapeutics Ribozymes is an unmatched resource for all biochemists, biotechnologists, molecular biologists, and bioengineers interested in the topic.

Author : Adrian Krainer
Publisher : IRL Press
Page : 408 pages
File Size : 42,7 Mb
Release : 1997
Category : Language Arts & Disciplines
ISBN : STANFORD:36105020129602

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Eukaryotic MRNA Processing by Adrian Krainer Pdf

This volume focuses on the major aspects of post-transcriptional mRNA processing in the nucleus of eukaryotic cells. Each of the described mRNA reactions is required for proper gene expression and can also serve as a control point for regulating the expression of many genes, for example duringembryonic development or in different cell types. The different chapters review the assembly of newly synthesized nuclear mRNA transcripts into hnRNP particles and catalytically active spliceosomes; the structure and mechanism of action of small nuclear ribonucleoprotein particles and proteinfactors that catalyse pre-mRNA splicing in mammalian cells and in yeast; the regulation of gene expression and generation of protein isoform diversity by alternative splicing; the mechanisms of 3' end cleavage and polyadenylation; the architecture of the cell nucleus in relation to these processesand to the localization of the relevant substrates and factors; the diverse mechanisms of RNA processing by ribozymes and their potential relevance for nuclear mRNA processing; the mechanism of spliced-leader addition by trans-splicing in nematodes and trypanosomes; and the process ofinsertion/deletion mRNA editing in kinetoplasmid protozoa. In each chapter, leading researchers have provided detailed, critical reviews of the history, experimental approaches, major advances, current ideas and models, as well as future directions, for each of these active areas of research.

Author : B. D. Hames,David M. Glover
Publisher : Oxford University Press, USA
Page : 238 pages
File Size : 44,7 Mb
Release : 1988
Category : Music
ISBN : UOM:39015013557494

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Transcription and Splicing by B. D. Hames,David M. Glover Pdf

This book gives a co-ordinated review of our present knowledge of eukaryotic RNA synthesis.

Author : Thomas R. Cech,Joan A. Steitz,John F. Atkins
Publisher : Unknown
Page : 580 pages
File Size : 54,8 Mb
Release : 2018-12-31
Category : Science
ISBN : 1621822249

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RNA Worlds: New Tools for Deep Exploration by Thomas R. Cech,Joan A. Steitz,John F. Atkins Pdf

"A Subject Collection from Cold Spring Harbor Perspectives in Biology."

Author : Toshio Nakanishi,H. Scott Baldwin,Jeffrey R. Fineman,Hiroyuki Yamagishi
Publisher : Springer Nature
Page : 374 pages
File Size : 44,8 Mb
Release : 2020-02-28
Category : Medical
ISBN : 9789811511851

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Molecular Mechanism of Congenital Heart Disease and Pulmonary Hypertension by Toshio Nakanishi,H. Scott Baldwin,Jeffrey R. Fineman,Hiroyuki Yamagishi Pdf

This open access book focuses on the molecular mechanism of congenital heart disease and pulmonary hypertension, offering new insights into the development of pulmonary circulation and the ductus arteriosus. It describes in detail the molecular mechanisms involved in the development and morphogenesis of the heart, lungs and ductus arteriosus, covering a range of topics such as gene functions, growth factors, transcription factors and cellular interactions, as well as stem cell engineering technologies. The book also presents recent advances in our understanding of the molecular mechanism of lung development, pulmonary hypertension and molecular regulation of the ductus arteriosus. As such, it is an ideal resource for physicians, scientists and investigators interested in the latest findings on the origins of congenital heart disease and potential future therapies involving pulmonary circulation/hypertension and the ductus arteriosus.

Author : Philippe Jeanteur
Publisher : Springer Science & Business Media
Page : 254 pages
File Size : 52,5 Mb
Release : 2013-03-14
Category : Science
ISBN : 9783662097281

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Regulation of Alternative Splicing by Philippe Jeanteur Pdf

The discovery in 1977 that genes are split into exons and introns has done away with the one gene - one protein dogma. Indeed, the removal of introns from the primary RNA transcript is not necessarily straightforward since there may be optional pathways leading to different messenger RNAs and consequently to different proteins. Examples of such an alternative splicing mechanism cover all fields of biology. Moreover, there are plenty of occurrences where deviant splicing can have pathological effects. Despite the high number of specific cases of alternative splicing, it was not until recently that the generality and extent of this phenomenon was fully appreciated. A superficial reading of the preliminary sequence of the human genome published in 2001 led to the surprising, and even deceiving to many scientists, low number of genes (around 32,000) which contrasted with the much higher figure around 150,000 which was previously envisioned. Attempts to make a global assessment of the use of alternative splicing are recent and rely essentially on the comparison of genomic mRNA and EST sequences as reviewed by Thanaraj and Stamm in the first chapter of this volume. Most recent estimates suggest that 40-60% of human genes might be alternatively spliced, as opposed to about 22% for C. elegans.

Author : Peter Scheiffele,Oriane Mauger
Publisher : Springer Nature
Page : 356 pages
File Size : 47,8 Mb
Release : 2022-07-27
Category : Science
ISBN : 9781071625217

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Alternative Splicing by Peter Scheiffele,Oriane Mauger Pdf

This detailed volume collects commonly used and cutting-edge methods to analyze alternative splicing, a key step in gene regulation. After an introduction of the alternative splicing mechanism and its targeting for therapeutic strategies, the book continues with techniques for analyzing alternative splicing profiles in complex biological systems, visualizing and localizing alternative spliced transcripts with cellular and sub-cellular resolution, probing regulators of alternative splicing, as well as assessing the functional consequences of alternative splicing. Written for the highly successful Methods in Molecular Biology series, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Alternative Splicing: Methods and Protocols serves as an ideal guide for both RNA aficionados that want to implement novel approaches in their labs and novices undertaking alternative splicing projects.

Author : Katharine Marie Lang
Publisher : Unknown
Page : 308 pages
File Size : 48,8 Mb
Release : 1987
Category : Electronic
ISBN : WISC:89014501407

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The Splicing of Precursor MRNAs Containing Multiple Intervening Sequences by Katharine Marie Lang Pdf

Author : Philippe Jeanteur
Publisher : Springer Science & Business Media
Page : 265 pages
File Size : 53,7 Mb
Release : 2006-10-04
Category : Science
ISBN : 9783540344490

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Alternative Splicing and Disease by Philippe Jeanteur Pdf

Splicing of primary RNA transcript is a quasi-systematic step of gene expression in higher organisms. This is the first book to highlight the medical implications, i.e. diseases, caused by alternative splicing. Alternative splicing not only vastly increases protein diversity but also offers numerous opportunities for aberrant splicing events with pathological consequences. The book also outlines possible targets for therapy.

Author : Zbigniew Stanisław Warkocki
Publisher : Unknown
Page : 161 pages
File Size : 44,8 Mb
Release : 2012
Category : Electronic
ISBN : 3868444394

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Reconstitution of Both Steps of Yeast Pre-mRNA Splicing in Vitro with Purified Components by Zbigniew Stanisław Warkocki Pdf

Author : Witold Filipowicz,Thomas Hohn
Publisher : Springer Science & Business Media
Page : 419 pages
File Size : 43,7 Mb
Release : 2012-12-06
Category : Science
ISBN : 9789400903531

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Post-Transcriptional Control of Gene Expression in Plants by Witold Filipowicz,Thomas Hohn Pdf

A recent volume of this series (Signals and Signal Transduction Pathways in Plants (K. Palme, ed.) Plant Molecular Biology 26, 1237-1679) described the relay races by which signals are transported in plants from the sites of stimuli to the gene expression machinery of the cell. Part of this machinery, the transcription apparatus, has been well studied in the last two decades, and many important mechanisms controlling gene expression at the transcriptional level have been elucidated. However, control of gene expression is by no means complete once the RNA has been produced. Important regulatory devices determine the maturation and usage of mRNA and the fate of its translation product. Post-transcriptional regulation is especially important for generating a fast response to environmental and intracellular signals. This book summarizes recent progress in the area of post-transcriptional regulation of gene expression in plants. 18 chapters of the book address problems of RNA processing and stability, regulation of translation, protein folding and degradation, as well as intracellular and cell-to-cell transport of proteins and nucleic acids. Several chapters are devoted to the processes taking place in plant organelles.

Author : Anonim
Publisher : Academic Press
Page : 365 pages
File Size : 45,8 Mb
Release : 2014-08-27
Category : Science
ISBN : 9780128013199

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Brain Transcriptome by Anonim Pdf

Published since 1959, International Review of Neurobiology is a well-known series appealing to neuroscientists, clinicians, psychologists, physiologists, and pharmacologists. Led by an internationally renowned editorial board, this important serial publishes both eclectic volumes made up of timely reviews and thematic volumes that focus on recent progress in a specific area of neurobiology research. This volume, concentrates on the brain transcriptome. Brings together cutting-edge research on the brain transcriptome