Freeze Etch Histology

Author : L. Orci,A. Perrelet
Publisher : Springer Science & Business Media
Page : 149 pages
File Size : 51,6 Mb
Release : 2012-12-06
Category : Medical
ISBN : 9783642660207

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Freeze-Etch Histology by L. Orci,A. Perrelet Pdf

The development of the electron microscope brought one to the other. The purpose of this book is to in a new era, in which cell structures could be provide a bridge, to help those who are familiar visualized down to a macromolecular level. It is of with conventional electron microscopy, to cross course well recognized that some cell components into the less familiar freeze-etching. This has been can be modified or even completely lost during the done by providing paired photographs wherever complex sequence of fixation, dehydration, staining possible, one showing the thin section of a tissue or and plastic embedding which is essential before a a cell, the other a freeze-etch replica of a similar thin section can be obtained. Further, only these cell material. At the same time we have tried to provide components which can be made electron opaque a basis for interpretation of freeze-etched structures, can be visualized. For these reasons, the morpho and we hope the reader will find the first 23 Plates logist and cell biologist alike have to explore alter especially useful in this respect. Because there are native techniques of specimen preparation which many excellent atlases of cell structure available at may avoid some of the artefacts inherent in con the present time, we felt there was no need to ventional thin-section electron microscopy, and at provide lengthy discussions of each plate.

Author : Sek Wen Hui
Publisher : CRC Press
Page : 375 pages
File Size : 46,8 Mb
Release : 2018-01-18
Category : Medical
ISBN : 9781351089050

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Freeze-Fracture Studies of Membranes by Sek Wen Hui Pdf

This volume is a kind of celebration of the progress of freeze-fracture electron microscopy in recent years. Many of the authors are leaders of the advancing front. Instead of offering an instruction manual of how to perform new techniques or a review to recover what has happened in the past in respect fields, both which are abundantly available as journal articles and monographs, this volume is a collection of personal testimonies as examples of the power of the new freeze-fracture technology. Since each chapter also centres around specific biological problem, it also serves to illustrate how much the understanding of the problem has been advanced by the new freeze-fracture methodology, which is most cases is developed by the author(s) themselves. A characteristic of frontier development is that many chapters are dealing with controversial subjects. The Inclusion of these subjects in the volume represents the dynamic nature of the subject as viewed by the authors rather than the final verdicts of the subject matter.

Author : C. Stolinski,A. S. Breathnach
Publisher : Unknown
Page : 192 pages
File Size : 51,7 Mb
Release : 1975
Category : Medical
ISBN : UOM:39015000286024

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Freeze-fracture Replication of Biological Tissues by C. Stolinski,A. S. Breathnach Pdf

Author : Nicholas J. Severs,David M. Shotton
Publisher : Wiley-Liss
Page : 392 pages
File Size : 47,5 Mb
Release : 1995-08-11
Category : Medical
ISBN : UOM:39015037496695

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Rapid Freezing, Freeze Fracture, and Deep Etching by Nicholas J. Severs,David M. Shotton Pdf

The techniques of freeze fracture and deep etching—techniques for the examination of the ultrastructure of rapidly frozen biological specimens by transmission electron microscopy—have profoundly influenced our understanding of the functional organization of the cell. Rapid Freezing, Freeze Fracture, and Deep Etching guides the reader through the principles of these techniques and gives detailed examples of their application in biomedical research. The book is organized into three sections. The first covers the fundamentals of freeze fracture and deep etching, with a discussion of basic principles for those new to the field, and an entire chapter on the equipment currently available for freeze fracture. Advanced techniques are presented next, with each chapter devoted to an in-depth treatment of a specific topic, including the advantages and limitations of each technique. The final chapters offer a series of applications selected to illustrate the wide-ranging and enduring impact of rapid freezing, freeze fracture, and deep etching on the field of biomedical research. With over 150 illustrations and containing contributions by leading experts instrumental in the development of these techniques, Rapid Freezing, Freeze Fracture, and Deep Etching offers authoritative coverage of such novel topics as: Hyperbaric freezing Ultrarapid freezing techniques in the study of dynamic cellular processes Cytochemical methods utilizing lectins and antibodies for labeling specific cellcomponents Fracturing and shadowing under ultra-low temperature and ultra-high vacuum conditions Freeze fracture and deep etching in neurobiology Rapid Freezing, Freeze Fracture, and Deep Etching will appeal to both newcomers to the field and experienced researchers and will be essential reading for cell biologists and anatomists, as well as any researcher who uses electron microscopy.

Author : M. A. Hayat
Publisher : Unknown
Page : 312 pages
File Size : 51,7 Mb
Release : 1970
Category : Science
ISBN : UOM:39015049446621

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Principles and Techniques of Electron Microscopy by M. A. Hayat Pdf

Freeze-substitution and freeze-drying; Freezing technology; The freeze-etching technique; Freeze-etching methodology; Intepretation of freeze-etching images; Negative staining; Equipment requirements; Negative staining; Summary comments on negative staining procedures; Photography of negatively stained specimens; Particle dimensions and molecular weights; Shadow casting and replication; High resolution and shadowing; Autoradiography; Conditions for quantitation; Analysis of autoradrograms; Technical considerations.

Author : R.V. Krstic;
Publisher : Springer Science & Business Media
Page : 460 pages
File Size : 51,6 Mb
Release : 2012-12-06
Category : Medical
ISBN : 9783642515965

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Illustrated Encyclopedia of Human Histology by R.V. Krstic; Pdf

During the past three decades, histology has seen enormous progress, thanks to new techniques and new investigation instruments. Numerous discoveries of important structures and morphofunctional phenomena have been described in a wealth of papers of ever increasing size and complexity. These publi cations have become difficult to follow, not only because of their number, but also because of a disparity of terminology and the multitude of synonyms employed by different authors. All of this makes reading and comprehension of the progress that has been achieved laborious, even for histologists, but especially for students, researchers in other basic branches of medicine, or clinicians who have to consult histological texts during their studies or investigations. In order to facilitate the orientation of all those interested in histology, a concise and practical volume in encyclopedic style, defining and, at the same time, illustrating fundamental histolog ical terms, enumerating synonyms, and describing morpho functional phenomena has become necessary, both because a work of this genre does not exist and because the list of Latin terms elaborated by the International Anatomical Nomenclature Committee in 1977 does not give illustrations or definitions of histological terms. The present work attempts to redress this deficiency.

Author : Donald B. McMillan,Richard James Harris
Publisher : Academic Press
Page : 634 pages
File Size : 48,5 Mb
Release : 2018-06-04
Category : Medical
ISBN : 9780124095977

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An Atlas of Comparative Vertebrate Histology by Donald B. McMillan,Richard James Harris Pdf

Atlas of Comparative Vertebrate Histology looks at the histology of a wide range of vertebrates, representative of all the major classes and families, with examples ranging from amphioxus to primates. The authors focus their microscope on commonly seen vertebrates as well as ‘non-standard’ species, such as lamprey, hagfish, dogfish, skate, rock bass, cod, river catfish, toad, amphiuma, leopard and bull frog, garter and brown snake, Coturnix quail and cowbird. The study of comparative histology in the vertebrates helps students and researchers alike understand how various groups have addressed similar problems, opening doors to interesting research possibilities. Not all vertebrates follow the mammalian model of tissue and organ structure. When dealing with unique species, we see some structures taken beyond their ‘normal’ function. Comparative histology allows us to understand the structural responses underlying the physiology unique to each vertebrate group. Presents the histology of a wide range of vertebrates, representative of all the major classes and families, with examples ranging from amphioxus to primates Includes an image gallery with over 500 flat images and 50+ virtual microscopy slides Contains electronic content features cross linking between text, tables and the image gallery

Author : J. Kartenbeck,H. Zentgraf,U. Scheer,W. W. Franke
Publisher : Springer Science & Business Media
Page : 55 pages
File Size : 53,9 Mb
Release : 2013-06-29
Category : Medical
ISBN : 9783662103906

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The Nuclear Envelope in Freeze-Etching by J. Kartenbeck,H. Zentgraf,U. Scheer,W. W. Franke Pdf

During the past twenty years the structure of the nuclear envelope, and in particular, that of its most distinct elements, the nuclear pore complexes, has been described from thin section electron microscopy (e, g., Brettschneider, 1952; Hartmann, 1953; Bahr and Beermann, 1954; Watson, 1954; Kautz and de Marsh, 1955; Watson, 1955), from metal-shadowed (e. g., Callan and Tomlin, 1950; Gall, 1954, 1956) and negatively stained (e. g., Franke, 1966, 1967; Gall, 1967; Yoo and Bayley, 1967) preparations of isolated nuclear membranes as revealing characte ristics common to euka. ryotic cells in general (recently reviewed, e. g., in Gouran ton, 1969; Stevens and Andre, 1969; Franke, 1970). In the recent years the freeze-etch technique (Steere, 1957) has proved to be a particularly useful tool in studying membraneous structures (e. g., Moor and Miihlethaler, 1963; Branton and Moor, 1964; Branton, 1966; Koehler, 1968b; Staehelin, l968a; Northcote, 1968a; Branton, 1969; Moor, 1969a). So this method has especially broadened the knowledge, e. g., on bacterial membranes (Bayer and Remsen, 1970; Nanninga, 1970), on erythrocyte plasma membranes (Weinstein and Bullivant, 1967; Meyer and Winkelmann, 1970; da Silva and Branton, 1970; Tillack and Marchesi, 1970), on liver cell membranes (Chalcroft and Bullivant, 1970), on Golgi membranes (Werz and Kellner, 1970; Staehelin and Kiermayer, 1970), on synaptic vesicles (Moor et al.

Author : C. Heym,W.-G. Forssmann
Publisher : Springer Science & Business Media
Page : 405 pages
File Size : 55,9 Mb
Release : 2012-12-06
Category : Medical
ISBN : 9783642680298

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Techniques in Neuroanatomical Research by C. Heym,W.-G. Forssmann Pdf

"Anatomy is the mother of physiology" - this statement was used to characterize the evolution of physiology from anato my as an independent science in the late nineteenth century. It had particular truth for neurophysiology, which started as functional neuroanatomy based on the observation of changes in behaviour after lesions of the nervous system both in ex perimental animals and in human patients. Today, anatomy may again be considered the mother of physiology; however, the meaning of this statement is rather different from that 100 years ago: The modem mother provides a dwelling for an increasing number of children endowed with new functional capabilities. This book provides a good illustration of such se mantic metamorphosis in the case of neuroanatomy. After a long period of little progress in either macroscopic neuroanatomy or neurohistology, during which the heritage of Cajal, Golgi, and others was developed and refined to yield a functional concept of the nervous system, the past two de cades have seen tremendous progress in methods applicable to the analysis of the nervous system. The new era was heralded by the introduction of the electron microscope to investigate the nervous system. This book is an impressive witness to the more recent developments.

Author : Leslie P. Gartner
Publisher : Elsevier Health Sciences
Page : 668 pages
File Size : 54,8 Mb
Release : 2020-01-23
Category : Medical
ISBN : 9780323672740

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Textbook of Histology by Leslie P. Gartner Pdf

Textbook of Histology, 5th Edition, brings you up to date with all that’s new in the field, while providing a solid foundation in the basic science and clinical application of cellular and molecular biology. Concise and highly illustrated, it functions as both a text and a histology laboratory guide and remains the only histology textbook that includes laboratory exercises for nearly every chapter. Numerous new clinical observations illustrate the importance of histology to clinical practice. More than 170 photomicrographs as well as new drawings, and histology laboratory instructions in most chapters have been added to this edition. Greatly revised content includes new findings in cellular and molecular biology such as the newly discovered endoplasmic reticulum-shaping proteins, the abundance of stem cells in adipose tissue, the phases of Alzheimer’s disease and the role of the newly discovered glymphatic system in slowing the progression of the disease, and developments in the microbiome. More quick-reference tables have been added to summarize information discussed in the text. PowerPointTM presentations, bolded key terms, bulleted lists, and chapter summaries highlight "need to know" information and facilitate comprehension A combination of USMLE-style questions and image-based questions are found in each chapter of the digital edition

Author : Douglas E. Chandler,Robert W. Roberson
Publisher : Jones & Bartlett Publishers
Page : 468 pages
File Size : 48,7 Mb
Release : 2009
Category : Medical
ISBN : 9780763738747

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Bioimaging by Douglas E. Chandler,Robert W. Roberson Pdf

The Development Of Microscopy Revolutionized The World Of Cell And Molecular Biology As We Once Knew It And Will Continue To Play An Important Role In Future Discoveries. Bioimaging: Current Concepts In Light And Electron Microscopy Is The Optimal Text For Any Undergraduate Or Graduate Bioimaging Course, And Will Serve As An Important Reference Tool For The Research Scientist. This Unique Text Covers, In Great Depth, Both Light And Electron Microscopy, As Well As Other Structure And Imaging Techniques Like X-Ray Crystallography And Atomic Force Microscopy. Written In A User-Friendly Style And Covering A Broad Range Of Topics, Bioimaging Describes The State-Of-The-Art Technologies That Have Powered The Field To The Forefront Of Cellular And Molecular Biological Research.

Author : C. Stolinski
Publisher : Unknown
Page : 173 pages
File Size : 41,9 Mb
Release : 1975
Category : Freeze fracturing
ISBN : OCLC:969343617

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Freeze-fracture Replication of Biological Tissues by C. Stolinski Pdf

Author : Anonim
Publisher : Elsevier
Page : 369 pages
File Size : 45,6 Mb
Release : 1977-01-01
Category : Medical
ISBN : 0080861717

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Membrane Morphology of the Vertebrate Nervous System by Anonim Pdf

Membrane Morphology of the Vertebrate Nervous System

Author : Richard L. Roberts,Richard G. Kessel,Hai-Nan Tung
Publisher : Oxford University Press, USA
Page : 430 pages
File Size : 53,9 Mb
Release : 1991
Category : Electron microscopy
ISBN : 0195052285

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Freeze Fracture Images of Cells and Tissues by Richard L. Roberts,Richard G. Kessel,Hai-Nan Tung Pdf

The freeze fracture technique coupled with transmission electron microscopy has contributed significant scientific information toward the experimental investigation of both normal and abnormal cells in the fields of cellular, developmental and molecular biology, and to several subdisciplines in medicine, including pathology, anatomy, and physiology. This book presents a complete and up-to-date account of the macromolecular organization of membranes and the many membrane specialization of cells as well as overall cellular organization as reflected in tissues and organs. While the book emphasizes freeze fracture images and the useful scientific information contained in them, the authors have also included transmission electron micrographs of ultrathin sectioned cells, tissues, and organs in order to aid in the interpretation of the freeze fracture image and increase the book's utility. Where three dimensional views are particularly useful, scanning electron micrographs are included.

Author : Anonim
Publisher : Academic Press
Page : 327 pages
File Size : 52,5 Mb
Release : 1983-01-07
Category : Science
ISBN : 0080586163

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International Review of Cytology by Anonim Pdf

International Review of Cytology