Separations For Biotechnology 2

Author : D. Leo Pyle
Publisher : Springer Science & Business Media
Page : 659 pages
File Size : 49,5 Mb
Release : 2012-12-06
Category : Science
ISBN : 9789400907836

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Separations for Biotechnology 2 by D. Leo Pyle Pdf

The challenge of bioseparations is to isolate and purify identified products from the dilute product broth produced from cell culture. Innovation in bioseparations technology is increasingly driven by the requirements imposed by the growing importance of production on a process scale of injectable-grade products, and economic pressures to improve the efficiency of downstream processing. As in other areas of technical change, science does not necessarily precede new technology: progress results from a complex and messy mixture of advances in understanding, ingenious ideas, novel techniques and chance discoveries. What is certain is that close interaction between academics and practitioners, biological scientists and process engineers is needed to solve the problems of bioseparations. The Second International Conference on Separations for Biotechnology at Reading, UK, in September 1990 set out to provide a critical multidisciplinary forum for the discussion of bioseparations. This volume contains the papers presented at the meeting. The meeting was organised around six themes with oral and poster presentations on the science and practice of bioseparations technology, and the same structure has been kept for this book. We have also included the texts of the keynote review paper by Professor Alan Michaels and the introductory review papers specially commissioned for the conference. Within each part of this book the review paper is followed by the contributed papers grouped alphabetically by their first author. All the original papers published here were accepted for publication after scientific refereeing.

Author : Wallace Woon-Fong Leung
Publisher : Butterworth-Heinemann
Page : 503 pages
File Size : 44,8 Mb
Release : 2020-03-13
Category : Technology & Engineering
ISBN : 9780081026359

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Centrifugal Separations in Biotechnology by Wallace Woon-Fong Leung Pdf

Centrifugal Separations in Biotechnology, Second Edition, is the only book on the market devoted to centrifugal separation in biotechnology. Key topics covered include a full introduction to centrifugation, sedimentation and separation; detailed coverage of centrifuge types, including batch and semi-batch centrifuges, disk-stack and tubular decanter centrifuges; methods for increasing solids concentration; laboratory and pilot testing of centrifuges; selection and sizing centrifuges; scale-up of equipment, performance prediction and analysis of test results using numerical simulation. Centrifugal Separations in Biotechnology, Second Edition, provides guidance on troubleshooting and optimizing centrifuges, and then goes on to explore the commercial applications of centrifuges in biotechnology. It gives detailed process information and data to assist in the development of particular processes from existing systems. It is of value to professionals in the chemical, bioprocess, and biotech sectors, and all those concerned with bioseparation, bioprocessing, unit-operations and process engineering. Provides a comprehensive guide to centrifuges, their optimal development, and their operation in the biotechnology industry Updated throughout based on developments in industrial applications and advances in our understanding of centrifugal separations in biotechnology Discusses applications for the separation of proteins, DNA, mitochondria, ribosomes, lysosomes and other cellular elements Includes new sections on use of optimal polymer dosage in waste treatment, new centrifuge designs for applications in algae processing, biopharma, and more

Author : Anonim
Publisher : Unknown
Page : 128 pages
File Size : 45,7 Mb
Release : 1990
Category : Electronic
ISBN : OCLC:258603167

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Separations for biotechnology : international conference ; 2 by Anonim Pdf

Author : G. Street
Publisher : Springer Science & Business Media
Page : 248 pages
File Size : 54,5 Mb
Release : 1994-08-31
Category : Science
ISBN : 9780751400519

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Highly Selective Separations in Biotechnology by G. Street Pdf

Success in meeting the challenge to produce the commercial products anticipated by the exploitation of biological processes depends upon provid ing effective separation protocols. Effectiveness can be measured in terms of selectivity, purity, resolution and validatory success. The major processing problems are associated with either the selective recovery of molecules which are present in low concentrations from complex mixtures or the selective removal of contaminants from the desired molecule. Central to the evolution of processes satisfying this demand are the regulatory requirements being imposed by governments on the purity of a product, especially in the health care market. Synthetic organic chemists are increasingly finding it advantageous to conduct one or more steps using either enzymic biotransformations where molecules with a single and consistent stereochemistry or chirality are required. The underlying princi ples behind the methods, techniques and processes currently being used and developed commercially rely upon the biospecific nature and properties of the desired molecule. When these factors are married to the more traditional techniques of precipitation, chromatography, liquid-liquid extraction and membrane processes, powerful tools emerge, allowing highly selective separations to be designed. The logical extension of these combinations is to apply genetic engineering techniques to influence the separations at a more fundamental and structural level by modifying the target protein at source, during its synthesis, to facilitate its separation in a given, selective manner, leading to the distinct possibility of producing 'designer' separation programmes.

Author : Jack G. Chirikjian
Publisher : Jones & Bartlett Learning
Page : 292 pages
File Size : 54,9 Mb
Release : 1995
Category : Animal cell biotechnology
ISBN : 0867208961

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Biotechnology: Genetic engineering, mutagenesis, separation technology by Jack G. Chirikjian Pdf

Vol. II The work presented in these two volumes is the collaborative effort of over twenty undergraduate science faculty, whose common goal was to develop a text of unique and flexible laboratory activities focusing on the theory and practice of biotechnology for undergraduate students. The books are designed to provide flexibility for easy integration into any course in the life sciences with an experimental emphasis.

Author : Rajni Hatti-Kaul
Publisher : Springer Science & Business Media
Page : 442 pages
File Size : 42,7 Mb
Release : 2008-02-05
Category : Science
ISBN : 9781592590285

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Aqueous Two-Phase Systems by Rajni Hatti-Kaul Pdf

A mixture of two polymers, or one polymer and a salt, in an aqueous medium separates into two phases: this phenomenon is useful in biotechn- ogy for product separations. Separation of biological molecules and particles in these aqueous two-phase systems (ATPS) was initiated over 40 years ago by P.-Å. Albertsson, and later proved to be of immense utility in biochemical and cell biological research. A boost in the application of ATPS was seen when problems of separations in biotechnology processes were encountered. Its simplicity, biocompatibility, and amenability to easy scaleup operations make the use of ATPS very attractive for large-scale bioseparations. Despite the advantages ATPS enjoys over other separation techniques, the application of two-phase systems has for a long time been confined to selected labora- ries. Recent years have, however, shown a trend in which increasing numbers of researchers employ two-phase partitioning techniques in both basic and applied research.

Author : D. L. Pyle
Publisher : Unknown
Page : 634 pages
File Size : 53,7 Mb
Release : 1994
Category : Science
ISBN : UCSD:31822009015603

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Separations for Biotechnology 3 by D. L. Pyle Pdf

Bioseparation is a technique by which the products of fermentations or biotransformations are recovered for subsequent use. This volume explores the technical problems posed by bioprocessing, and the importance of the recovery of pure biological products for product safety and quality.

Author : A S Grandison,Alistair S. Grandison,M J Lewis
Publisher : Woodhead Publishing
Page : 306 pages
File Size : 41,5 Mb
Release : 1996-01-15
Category : Medical
ISBN : 1855732874

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Separation Processes in the Food and Biotechnology Industries by A S Grandison,Alistair S. Grandison,M J Lewis Pdf

This book reviews methods and techniques for separating food components and products of the biotechnology industry. The introduction focuses on food composition and some of the conventional separation techniques. Subsequent chapters deal with each specific type or area of application individually and include information on the basic principles, industrial equipment available, commercial applications and an overview of research and development.

Author : Anonim
Publisher : Unknown
Page : 634 pages
File Size : 54,6 Mb
Release : 1994
Category : Biomolecules
ISBN : UOM:39015035249872

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Separations for Biotechnology by Anonim Pdf

Author : William K. Wang
Publisher : CRC Press
Page : 432 pages
File Size : 49,6 Mb
Release : 2001-04-03
Category : Science
ISBN : 9781482289886

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Membrane Separations in Biotechnology by William K. Wang Pdf

This text details the relationship between membrane technology and bioprocesses, discussing applications. This second edition refines and optimizes key features of the first edition - and features new illustrative case studies. The book examines advantages and disadvantages of using standard and new membrane technologies; analyzes a wide range of a

Author : Harry Walter
Publisher : Elsevier
Page : 730 pages
File Size : 42,7 Mb
Release : 2012-12-02
Category : Science
ISBN : 9780323142915

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Partitioning In Aqueous Two – Phase System by Harry Walter Pdf

Partitioning in Aqueous Two-Phase Systems: Theory, Methods, Uses, and Applications to Biotechnology is a collection of papers that discusses the applications of aqueous two-phase systems to problems of separation and extraction of macromolecules, organelles, and cells. Papers focus on the theoretical basis and the practical details of the procedures used. Some of the papers describe in one or a few steps how two components can be separated by the investigator manipulating their partitions so that one component is in one phase and the other component is in the other phase or at the interface. Investigators can also avail of developed batch extractions for plant organelles, cell membranes, nucleic acids, and proteins. The book cites as an example the partitioning of right-side-out and inside-out vesicles (obtained from fragments of thylakoid membranes) to the top and bottom phases, respectively, of a Dx-PEG system. Other papers describe the use of the countercurrent distribution when single extraction steps are not sufficient to produce a separation in materials that do not differ greatly in their partitioning behavior. The collection can prove valuable for bio-chemists, cellular biologists, micro-biologists, and developmental biologists.

Author : Juan A. Asenjo
Publisher : CRC Press
Page : 824 pages
File Size : 42,8 Mb
Release : 2020-08-26
Category : Science
ISBN : 9781000104325

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Separation Processes in Biotechnology by Juan A. Asenjo Pdf

Edited to avoid duplication and favor comprehensiveness, 20 contributors detail the recovery, separation, and purification operations of bioprocess technology. Individual chapters in this classic yet still highly relevant work emphasize concepts that are becoming more and more important when applied to the large scale versions of techniques that are considered well established. Aside from fully discussing processes, Separation Processes in Biotechnology includes sections on concentration separation and operation, purification operations, and product release and recovery. It also discusses plant operation and equipment and delves into economic considerations

Author : Juan A. Asenjo
Publisher : CRC Press
Page : 828 pages
File Size : 51,6 Mb
Release : 2020-08-26
Category : Science
ISBN : 9781000147537

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Separation Processes in Biotechnology by Juan A. Asenjo Pdf

Edited to avoid duplication and favor comprehensiveness, 20 contributors detail the recovery, separation, and purification operations of bioprocess technology. Individual chapters in this classic yet still highly relevant work emphasize concepts that are becoming more and more important when applied to the large scale versions of techniques that are considered well established. Aside from fully discussing processes, Separation Processes in Biotechnology includes sections on concentration separation and operation, purification operations, and product release and recovery. It also discusses plant operation and equipment and delves into economic considerations

Author : John Paul Frampton,Brendan M. Leung,Anderson Ho Cheung Shum
Publisher : Frontiers Media SA
Page : 117 pages
File Size : 53,5 Mb
Release : 2020-01-13
Category : Electronic
ISBN : 9782889633562

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The Science and Application of Aqueous Two-Phase Systems and Liquid-Liquid Phase Separation in Biotechnology and Bioengineering by John Paul Frampton,Brendan M. Leung,Anderson Ho Cheung Shum Pdf

The phase separation of incompatible liquids has been a topic of significant importance in chemical and industrial engineering for many years. Well-understood examples of this phenomenon include the phase separation of oil with water and the phase separation of non-polar organic solvents with water. Similar behavior is observed when aqueous solutions of two or more incompatible polymers or polymers and salts are mixed. In these mixtures (referred to as aqueous two-phase systems), the separated phases are composed mostly of water. Aqueous two-phase systems have been used extensively for the extraction of high-value biological products from mixtures of biological materials. In recent years, aqueous two-phase systems have also found increased use as materials for streamlining and improving the capabilities of cell and molecular assays, and for the design of advanced cell culture systems. Similar behavior of biological materials in living systems has also been observed, with emerging roles in cell physiology.

Author : Chirikjian
Publisher : Unknown
Page : 128 pages
File Size : 44,7 Mb
Release : 2009-06-01
Category : Electronic
ISBN : 8123917171

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Biotechnology Theory and Techniques, Vol. 2 (Genetic Engineering Mutagenesis Separation Technology) by Chirikjian Pdf