Microtubules In Vitro

Author : John J. Correia,Leslie Wilson
Publisher : Academic Press
Page : 472 pages
File Size : 51,6 Mb
Release : 2013-08-20
Category : Science
ISBN : 9780124078888

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Microtubules, in vitro by John J. Correia,Leslie Wilson Pdf

There continues to be intense interest in the microtubule cytoskeleton; the assembly, structure and regulation of microtubules; and the numerous motors and accessory proteins that control cell cycle, dynamics, organization and transport. The field continues to grow and explore new aspects of these issues driven immensely by developments in optical imaging and tracking techniques. This Second Edition brings together current research and protocols in the field of microtubules in vitro and will serve as a valuable tool for cell biologists, biophysicists and pharmacologists who study the microtubule cytoskeleton, as well as for researchers in the biomedical and biotechnology communities with interest in developing drugs that target microtubules, MAPS and motors. Chapters reflect experimental procedures and new developments in the field of microtubule in vitro research Combines classical approaches and modern technologies Presents easy-to-use protocols and thorough background information, compiled by leaders in the field

Author : Anonim
Publisher : Academic Press
Page : 552 pages
File Size : 55,7 Mb
Release : 2010-09-24
Category : Science
ISBN : 0123813506

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Microtubules: in vivo by Anonim Pdf

Microtubules: in vivo includes chapters by experts around the world on many aspects of microtubule imaging in living and fixed cells; assays to study microtubule function in a wide array of model organisms and cultured cells; high resolution approaches to study of the cytoskeleton. The authors share their years of experience, outlining potential pitfalls and critical factors to consider in experimental design, experimental implementation and data interpretation. Includes chapters by experts around the world on many aspects of microtubule imaging in living and fixed cells; assays to study microtubule function in a wide array of model organisms and cultured cells; high resolution approaches to study of the cytoskeleton The authors share their years of experience, outlining potential pitfalls and critical factors to consider in experimental design, experimental implementation and data interpretation

Author : Anne Straube
Publisher : Humana Press
Page : 319 pages
File Size : 48,7 Mb
Release : 2017-04-30
Category : Science
ISBN : 1493961853

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Microtubule Dynamics by Anne Straube Pdf

Microtubules are at the heart of cellular self-organization, and their dynamic nature allows them to explore the intracellular space and mediate the transport of cargoes from the nucleus to the outer edges of the cell and back. In Microtubule Dynamics: Methods and Protocols, experts in the field provide an up-to-date collection of methods and approaches that are used to investigate microtubule dynamics in vitro and in cells. Beginning with the question of how to analyze microtubule dynamics, the volume continues with detailed descriptions of how to isolate tubulin from different sources and with different posttranslational modifications, methods used to study microtubule dynamics and microtubule interactions in vitro, techniques to investigate the ultrastructure of microtubules and associated proteins, assays to study microtubule nucleation, turnover, and force production in cells, as well as approaches to isolate novel microtubule-associated proteins and their interacting proteins. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Definitive and practical, Microtubule Dynamics: Methods and Protocols provides the key protocols needed by novices and experts on how to perform a broad range of well-established and newly-emerging techniques in this vital field.

Author : John J. Correia
Publisher : Academic Press
Page : 0 pages
File Size : 41,5 Mb
Release : 2010-06-01
Category : Science
ISBN : 0123748151

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Microtubules, in vitro by John J. Correia Pdf

There continues to be intense interest in the microtubule cytoskeleton; the assembly, structure and regulation of microtubules; and the numerous motors and accessory proteins that control cell cycle, dynamics, organization and transport. The field continues to grow and explore new aspects of these issues driven immensely by developments in optical imaging and tracking techniques. This volume (complemented by the forthcoming companion volume by Cassimeris and Tran) brings together current research and protocols in the field of microtoubules in vitro and will serve as a valuable tool for cell biologists, biophysicists and pharmacologists who study the microtubule cytoskeleton, as well as for researchers in the biomedical and biotechnology communities with interest in developing drugs that target microtubules, MAPS and motors.

Author : Leslie Wilson,John J. Correia
Publisher : Academic Press
Page : 699 pages
File Size : 45,9 Mb
Release : 2010-07-03
Category : Science
ISBN : 0080888585

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Microtubules, in vitro by Leslie Wilson,John J. Correia Pdf

There continues to be intense interest in the microtubule cytoskeleton; the assembly, structure and regulation of microtubules; and the numerous motors and accessory proteins that control cell cycle, dynamics, organization and transport. The field continues to grow and explore new aspects of these issues driven immensely by developments in optical imaging and tracking techniques. This volume (complemented by the forthcoming companion volume by Cassimeris and Tran) brings together current research and protocols in the field of microtoubules in vitro and will serve as a valuable tool for cell biologists, biophysicists and pharmacologists who study the microtubule cytoskeleton, as well as for researchers in the biomedical and biotechnology communities with interest in developing drugs that target microtubules, MAPS and motors. * Chapters reflect both experimental procedures and new developments in the field of microtubule in vitro research. * Combines classical approaches and modern technologies * Presents easy-to-use protocols and thorough background information, compiled by leaders in the field

Author : Helder Maiato
Publisher : Unknown
Page : 390 pages
File Size : 46,8 Mb
Release : 2020
Category : Cytology
ISBN : 1071602195

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Cytoskeleton Dynamics by Helder Maiato Pdf

This volume details comprehensive state-of-the-art methods on actin microfilaments and microtubules and how they work to achieve different cellular functions in different cellular contexts. Chapters guide readers through protein purification, in vitro reconstitution of several cytoskeleton properties, analyses of microtubule- and actin-based structures, functional dissection of post-translational modifications, and roles in several biological processes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Cytoskeleton Dynamics: Methods and Protocols aims to provide a wide range of experimental approaches and be an invaluable resource for present and future generations of cytoskeleton researchers. .

Author : Qingqin Li
Publisher : Unknown
Page : 0 pages
File Size : 52,9 Mb
Release : 1995
Category : Electronic
ISBN : OCLC:1426859870

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In Vitro Interactions of Γ-tubulin and Microtubules by Qingqin Li Pdf

Author : Bruce Alberts
Publisher : Unknown
Page : 0 pages
File Size : 43,9 Mb
Release : 2002
Category : Cytology
ISBN : 0815332181

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Molecular Biology of The Cell by Bruce Alberts Pdf

Author : Anonim
Publisher : Academic Press
Page : 425 pages
File Size : 43,9 Mb
Release : 1982-08-04
Category : Science
ISBN : 0080859232

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The Cytoskeleton, Part B by Anonim Pdf

The Cytoskeleton, Part B

Author : Hiroshi Inaba
Publisher : Unknown
Page : 486 pages
File Size : 52,8 Mb
Release : 2022
Category : Biomedical engineering
ISBN : 1071619837

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Microtubules by Hiroshi Inaba Pdf

This volume provides state-of-the-art methodology to functionalize microtubules and construct microtubule-based materials. Chapters consists of six sections detailing modification of microtubule structures, observation and control of microtubule movement, material applications of microtubules in vitro, development of microtubule-binding molecules, comprehensive approaches to analyze properties of microtubules, and functionalization of microtubules in living cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Microtubules: Methods and Protocols aims to provide useful information for construction of microtubule-based materials and help to create new ideas for the next-generation of materials and applications through detailed protocols.

Author : Antonio Tito Fojo
Publisher : Springer Science & Business Media
Page : 629 pages
File Size : 48,5 Mb
Release : 2009-01-14
Category : Medical
ISBN : 9781597453363

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The Role of Microtubules in Cell Biology, Neurobiology, and Oncology by Antonio Tito Fojo Pdf

This book presents the first comprehensive exploration of the dynamic potential of microtubules anti-cancer targets. Written by leading anti-cancer researchers, this groundbreaking volume collects the most current microtubule research available and investigates the potential of microtubules in cancer therapy.

Author : Anonim
Publisher : Academic Press
Page : 552 pages
File Size : 48,8 Mb
Release : 2010-09-09
Category : Science
ISBN : 0123813492

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Microtubules: in vivo by Anonim Pdf

Microtubules: in vivo includes chapters by experts around the world on many aspects of microtubule imaging in living and fixed cells; assays to study microtubule function in a wide array of model organisms and cultured cells; high resolution approaches to study of the cytoskeleton. The authors share their years of experience, outlining potential pitfalls and critical factors to consider in experimental design, experimental implementation and data interpretation. Includes chapters by experts around the world on many aspects of microtubule imaging in living and fixed cells; assays to study microtubule function in a wide array of model organisms and cultured cells; high resolution approaches to study of the cytoskeleton The authors share their years of experience, outlining potential pitfalls and critical factors to consider in experimental design, experimental implementation and data interpretation

Author : Peter Nick
Publisher : Springer
Page : 269 pages
File Size : 54,8 Mb
Release : 2010-10-28
Category : Science
ISBN : 3642095887

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Plant Microtubules by Peter Nick Pdf

Since the publication of the first edition of Plant Microtubules in 2000, our understanding of microtubules and their manifold functions have advanced substantially. This revised edition highlights the morphogenetic potential of plant microtubules from three general viewpoints: Microtubules and Morphogenesis, Microtubules and Environment, Microtubules and Evolution. The book is an invaluable source of information for researchers as well as for graduate and advanced students.

Author : Brian L. Shigekawa
Publisher : Unknown
Page : 294 pages
File Size : 52,6 Mb
Release : 1978
Category : Cell physiology
ISBN : UCR:31210002392411

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The Phosphorylation State of Tubulin and Its Effect on in Vitro Microtubule Assembly and Aggregation Phenomena by Brian L. Shigekawa Pdf

Author : Jose C. Jimenez-Lopez
Publisher : BoD – Books on Demand
Page : 344 pages
File Size : 51,5 Mb
Release : 2017-05-17
Category : Science
ISBN : 9789535131694

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Cytoskeleton by Jose C. Jimenez-Lopez Pdf

The cytoskeleton is a highly dynamic intracellular platform constituted by a three-dimensional network of proteins responsible for key cellular roles as structure and shape, cell growth and development, and offering to the cell with "motility" that being the ability of the entire cell to move and for material to be moved within the cell in a regulated fashion (vesicle trafficking). The present edition of Cytoskeleton provides new insights into the structure-functional features, dynamics, and cytoskeleton's relationship to diseases. The authors' contribution in this book will be of substantial importance to a wide audience such as clinicians, researches, educators, and students interested in getting updated knowledge about molecular basis of cytoskeleton, such as regulation of cell vital processes by actin-binding proteins as cell morphogenesis, motility, their implications in cell signaling, as well as strategies for clinical trial and alternative therapies based in multitargeting molecules to tackle diseases, that is, cancer.