Brain Receptor Methodologies

Author : Paul J. Marangos
Publisher : Elsevier
Page : 384 pages
File Size : 44,6 Mb
Release : 2012-12-02
Category : Science
ISBN : 9780323152808

Get Book

Brain Receptor Methodologies Pt A by Paul J. Marangos Pdf

Brain Receptor Methodologies, Part A, General Methods and Concepts: Amines and Acetylcholine provides information pertinent to neurotransmitter and neuromodulator receptors in brain. This book explores the methodologies that can used to address several basic and clinical problems. Organized into two sections encompassing 18 chapters, this book starts with an overview of the receptor concept, which can be validated from indirect evidence obtained in studies of the quantitative aspects of drug antagonism. This text then examines the radioligand–receptor binding interactions. Other chapters consider immunocytochemistry, which has a primary role in determining the precise distribution of regulatory peptides to neural and endocrine elements of the diffuse neuroendocrine system. The final chapter discusses the use of the radioligand binding procedure for the study of muscarinic receptors, which has expanded the area of muscarinic receptor pharmacology. Biochemists, pharmacologists, physiologists, and researchers engaged in the fields of neurobiology and neuroscience will find this book extremely useful.

Author : Paul J. Marangos,Robert M. Cohen,Iain C. Campbell
Publisher : Unknown
Page : 392 pages
File Size : 48,9 Mb
Release : 1984
Category : Brain chemistry
ISBN : UOM:39015007102760

Get Book

Brain Receptor Methodologies by Paul J. Marangos,Robert M. Cohen,Iain C. Campbell Pdf

Brain Receptor Methodologies, Part A, General Methods and Concepts: Amines and Acetylcholine provides information pertinent to neurotransmitter and neuromodulator receptors in brain. This book explores the methodologies that can used to address several basic and clinical problems. Organized into two sections encompassing 18 chapters, this book starts with an overview of the receptor concept, which can be validated from indirect evidence obtained in studies of the quantitative aspects of drug antagonism. This text then examines the radioligand-receptor binding interactions. Other chapters consi ...

Author : Paul J. Marangos,Iain C. Campbell,Robert M. Cohen
Publisher : Academic Press
Page : 357 pages
File Size : 48,8 Mb
Release : 2013-10-22
Category : Nature
ISBN : 9781483269597

Get Book

Brain Receptor Methodologies by Paul J. Marangos,Iain C. Campbell,Robert M. Cohen Pdf

Brain Receptor Methodologies: Part B Amino Acids. Peptides. Psychoactive Drugs is the second of the two-part first volume of the Neurobiological Research series, which provides a comprehensive view of various subdisciplines within neurobiology. The first volume (Parts A and B) deals with the area of neurotransmitter and neuromodulator receptors in brain; future volumes will cover the subdisciplines of neuroanatomy, neurophysiology, brain-specific macromolecules, neurochemistry, and behavioral neurobiology. It is hoped that the series will be of equal value for both basic as well as clinical scientists Part B continues from Part A with the remainder of Section II, specific receptor binding methodologies. Subsection II,B deals with receptors for amino acids and neuropeptides and covers areas including GABA, glycine, carnosine, opiates, bombesin, CCK, TRH, and substance P. Amino acids probably represent the majority of brain neurotransmitter substances, at least relative to the amines and acetylcholine, although with the exception of GABA, the amino acids remain relatively uncharacterized in brain. Their further study should receive high priority.

Author : Paul J. Marangos,Iain C. Campbell,Robert M. Cohen
Publisher : Unknown
Page : 128 pages
File Size : 51,7 Mb
Release : 1984
Category : Electronic
ISBN : 012470350X

Get Book

Brain Receptor Methodologies by Paul J. Marangos,Iain C. Campbell,Robert M. Cohen Pdf

Author : Rafael Luján,Francisco Ciruela
Publisher : Unknown
Page : 128 pages
File Size : 45,8 Mb
Release : 2021
Category : Electronic books
ISBN : 107161522X

Get Book

Receptor and Ion Channel Detection in the Brain by Rafael Luján,Francisco Ciruela Pdf

This second edition volume expands on the previous edition with new information on the latest developments in the field, and the most up-to-date representative methodological information on molecular neuroanatomical and functional techniques that are currently used to study neurotransmitter receptors and ion channels in the brain. The chapters in this book are organized into three parts. Part One discusses molecular immunological and pharmacological aspects related to the detection of receptors and ion channels in the brain. Part Two explores the neuroanatomical techniques devoted to localizing receptors and ion channels within the central nervous system. Part Three looks at functional approaches devoted to ascertaining receptors and ion channels functionality in the brain. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Cutting-edge and comprehensive, Receptor and Ion Channels Detection in the Brain, Second Edition is a valuable resource that contains detailed molecular, cellular, neuroanatomical, and electrophysiological methods that will help researchers study neurotransmitter receptor and ion channel in any region of neuron of the brain.

Author : Rafael Luján,Francisco Ciruela
Publisher : Humana
Page : 0 pages
File Size : 46,7 Mb
Release : 2016-02-02
Category : Medical
ISBN : 149393063X

Get Book

Receptor and Ion Channel Detection in the Brain by Rafael Luján,Francisco Ciruela Pdf

Receptor and Ion Channel Detection in the Brain provides state-of-the-art and up-to-date methodological information on molecular, neuroanatomical and functional techniques that are currently used to study neurotransmitter receptors and ion channels in the brain. The chapters have been contributed by world-wide recognized neuroscientists who explain in an easy and detailed way well established and tested protocols embracing molecular, cellular, subcellular, anatomical and electrophysiological aspects of the brain. This comprehensive and practical manual is presented in a simple, step-by-step manner for laboratory use, and also offers unambiguous detail and key implementation advice that proves essential for successful results and facilitate choosing the best method for the target proteins under study. This work serves as a useful guide for young researchers and students in training as well as for neurologists and established scientists who wish to extend their repertoire of techniques.

Author : Marjorie Ariano
Publisher : John Wiley & Sons
Page : 276 pages
File Size : 44,7 Mb
Release : 1998-07-16
Category : Medical
ISBN : 0471195243

Get Book

Receptor Localization by Marjorie Ariano Pdf

The detection of neurotransmitter receptor locations and distribution densities within the central nervous system and peripheral tissues is receiving intense attention within the neuroscience research community. Neurotransmitter receptors, which receive the chemical signals sent from one neuron to another, are critical links in a highly complex information-processing chain. Pinpointing receptor sites and systems is crucial for understanding neurological function as well as dysfunction. It is also essential for understanding how receptors process information when impacted by such substances as heroin or nicotine, or when affected by neurodegenerative disease. Receptor Localization: Laboratory Methods and Procedures is the first user-friendly guide to the latest techniques and approaches being employed to examine the localization of neurotransmitter receptors in the central nervous system and peripheral tissues. It covers detection methods that are applicable to a wide variety of receptor systems, ranging from genes and ligands to in vito receptors in individuals; and to numerous receptor subtypes, such as nicotine, muscarine, tackykinins, dopamine, adenosine, and GABA. The standard laboratory "recipes" or "tricks" employed in these detection methods are fully discussed, as are the advantages and limitations of each procedure. With contributions from leading experts and extensively illustrated, this book: * Discusses receptor ligand binding methods using irreversible and reversible compounds * Presents antireceptor antisera technology using synthetic peptides and fusion proteins at both the cellular and subcellular resolution levels * Examines molecular assessments of receptors * Describes in situ hybridization, reverse transcriptase-PCR, and fluorescent in situ transcription * Covers new visualization paradigms * Includes physiological analysis of receptor function, cellular detection in the brain slice, and cultured neurons * Discusses the use of PET and SPECT to assess in vivo receptor distributions in animals and humans Receptor Localization: Laboratory Methods and Procedures is an invaluable guide for researchers in the related fields of neurology, biochemistry, and pharmacology. Its lucid descriptions of new detection methods, inclusion of experimental examples, and emphasis on how these experimental approaches are applicable to particular research areas will appeal to both the experienced researcher and novice investigator.

Author : Henry I. Yamamura,S. J. Enna,Michael J. Kuhar
Publisher : Raven Press (ID)
Page : 298 pages
File Size : 55,6 Mb
Release : 1990
Category : Medical
ISBN : UOM:39015017698328

Get Book

Methods in Neurotransmitter Receptor Analysis by Henry I. Yamamura,S. J. Enna,Michael J. Kuhar Pdf

Author : Paul J. Marangos,Iain C. Campbell,Robert M. Cohen
Publisher : Unknown
Page : 368 pages
File Size : 47,7 Mb
Release : 1984
Category : Brain chemistry
ISBN : UOM:39015007082921

Get Book

Brain Receptor Methodologies: pt. A. General methods and concepts. Amines and acetylcholine by Paul J. Marangos,Iain C. Campbell,Robert M. Cohen Pdf

Author : S.H. Parvez,M. Naoi,T. Nagatsu
Publisher : Elsevier
Page : 436 pages
File Size : 53,7 Mb
Release : 2013-10-22
Category : Science
ISBN : 9781483290225

Get Book

Methods in Neurotransmitter and Neuropeptide Research by S.H. Parvez,M. Naoi,T. Nagatsu Pdf

This two-part book provides the most advanced biochemical and histochemical analytical methods on neurotransmitters, neuromodulators, and neuropeptides described by international specialists. The biochemical methods include liquid chromatography/electrochemistry, liquid chromatography/fluorescence and chemiluminescence detection, gas chromatography or liquid chromatography with mass spectrometry, and immunoassays. Morphological and physiological methods are also described, including immunohistochemistry, in situ hybridization, in vivo microdialysis, electrophysiological analysis, microwave irradiation, electrophysiological analysis, micro-anatomical dissection, peptide sequence analysis, quantitative autoradiographic analysis, and isolated CNS preparations. The neurotransmitters and neuropeptides include catecholamines, indoleamines, histamine, polyamines, and various neuropeptides, and their related enzymes. The book is the most comprehensive, advanced and versatile volume on the biochemical, morphological and physiological analysis of neurotransmitters and neuropeptides.

Author : M. E. Vartani͡an
Publisher : Unknown
Page : 180 pages
File Size : 49,8 Mb
Release : 1988
Category : Ligands
ISBN : UCAL:B4525881

Get Book

Neuronal Receptors, Endogenous Ligands, and Biotechnical Approaches by M. E. Vartani͡an Pdf

Author : Najam Arfeen Sharif,Michael Edward Lewis
Publisher : Unknown
Page : 316 pages
File Size : 53,9 Mb
Release : 1989
Category : Medical
ISBN : UOM:39015014489374

Get Book

Brain Imaging by Najam Arfeen Sharif,Michael Edward Lewis Pdf

Author : James A. Ferrendelli,Bruce S. McEwen,Solomon H. Snyder,Gerry Gurvitch
Publisher : Unknown
Page : 170 pages
File Size : 43,5 Mb
Release : 1976
Category : Aminobutyric acid
ISBN : UOM:39015003793877

Get Book

Neurotransmitters, Hormones, and Receptors by James A. Ferrendelli,Bruce S. McEwen,Solomon H. Snyder,Gerry Gurvitch Pdf

Author : Anthony P. Davenport
Publisher : Springer Science & Business Media
Page : 246 pages
File Size : 53,9 Mb
Release : 2008-02-02
Category : Medical
ISBN : 9781592599271

Get Book

Receptor Binding Techniques by Anthony P. Davenport Pdf

A comprehensive collection of readily reproducible methods for studying receptors in silico, in vitro, and in vivo. These cutting-edge techniques cover mining from curated databases, identifying novel receptors by high throughput screening, molecular methods to identify mRNA encoding receptors, radioligand binding assays and their analysis, quantitative autoradiography, and imaging receptors by positron emission tomography (PET). Highlights include phenotypic characterization of receptors in knockout mice, imaging receptors using green fluorescent protein and fluorescent resonance energy transfer, and quantitative analysis of receptor mRNA by TaqMan PCR. These book equips the researcher with techniques for exploring the unprecedented number of new receptor systems now emerging and the so-called "orphan" receptors whose activating ligand has not been identified.

Author : Arne Schousboe,Hans Bräuner-Osborne
Publisher : Springer Science & Business Media
Page : 282 pages
File Size : 51,5 Mb
Release : 2003-12-22
Category : Medical
ISBN : 9781592596720

Get Book

Molecular Neuropharmacology by Arne Schousboe,Hans Bräuner-Osborne Pdf

A powerful collection of readily reproducible cutting-edge techniques for characterizing the ligand or substrate binding of neurotransmitter receptors and transporters. The procedures cover interdisciplinary interactions for monoamine transporters, amino acid transporters, ionotropic receptors, metabotropic glutamate receptors, GABA receptors, and other G protein-coupled receptors. By illuminating how neurons in the central nervous communicate with other, these techniques can lead to the development of novel therapeutic strategies for neurological diseases.